The Korean Journal of Physiology and Pharmacology

  • 제21권1호
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  • Pages.99-106
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  • 2017
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  • 1226-4512(pISSN)
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  • 2093-3827(eISSN)
대한약리학회 (The Korean Society of Pharmacology)
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High fat diet confers vascular hyper-contractility against angiotensin II through upregulation of MLCK and CPI-17

  • Kim, Jee In (Department of Molecular Medicine, Keimyung University School of Medicine)
  • 투고 : 2016.09.21
  • 심사 : 2016.11.24
  • 발행 : 2017.01.01
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초록

Obesity is a critical risk factor for the hypertension. Although angiotensin II (Ang II) in obese individuals is known to be upregulated in obesity-induced hypertension, direct evidence that explains the underlying mechanism for increased vascular tone and consequent increase in blood pressure (BP) is largely unknown. The purpose of this study is to investigate the novel mechanism underlying Ang II-induced hyper-contractility and hypertension in obese rats. Eight-week old male Sprague-Dawley rats were fed with 60% fat diet or normal diet for 4 months. Body weight, plasma lipid profile, plasma Ang II level, BP, Ang II-induced vascular contraction, and expression of regulatory proteins modulating vascular contraction with/without Ang II stimulation were measured. As a result, high fat diet (HFD) accelerated age-dependent body weight gaining along with increased plasma Ang II concentration. It also increased BP and Ang II-induced aortic contraction. Basal expression of p-CPI-17 and myosin light chain (MLC) kinase was increased by HFD along with increased phosphorylation of MLC. Ang II-induced phosphorylation of CPI-17 and MLC were also higher in HFD group than control group. In conclusion HFD-induced hypertension is through at least in part by increased vascular contractility via increased expression and activation of contractile proteins and subsequent MLC phosphorylation induced by increased Ang II.

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참고문헌

  1. Abelson P, Kennedy D. The obesity epidemic. Science. 2004;304:1413. https://doi.org/10.1126/science.304.5676.1413
  2. Mark AL, Correia M, Morgan DA, Shaffer RA, Haynes WG. Stateof-the-art-lecture: Obesity-induced hypertension: new concepts from the emerging biology of obesity. Hypertension. 1999;33:537-541. https://doi.org/10.1161/01.HYP.33.1.537
  3. Goodfriend TL, Calhoun DA. Resistant hypertension, obesity, sleep apnea, and aldosterone: theory and therapy. Hypertension. 2004;43:518-524. https://doi.org/10.1161/01.HYP.0000116223.97436.e5
  4. Gu P, Xu A. Interplay between adipose tissue and blood vessels in obesity and vascular dysfunction. Rev Endocr Metab Disord. 2013;14:49-58. https://doi.org/10.1007/s11154-012-9230-8
  5. Uemura K, Mori N. Influence of age and sex on high-fat dietinduced increase in blood pressure. Nagoya J Med Sci. 2006;68:109-114.
  6. Davies MR, Gleich K, Katerelos M, Lee M, Mount PF, Power DA. The Thiazide-Sensitive Co-Transporter Promotes the Development of Sodium Retention in Mice with Diet-Induced Obesity. Kidney Blood Press Res. 2015;40:509-519. https://doi.org/10.1159/000368527
  7. Vargas-Robles H, Rios A, Arellano-Mendoza M, Escalante BA, Schnoor M. Antioxidative diet supplementation reverses high-fat diet-induced increases of cardiovascular risk factors in mice. Oxid Med Cell Longev. 2015;2015:467471.
  8. Boustany-Kari CM, Gong M, Akers WS, Guo Z, Cassis LA. Enhanced vascular contractility and diminished coronary artery flow in rats made hypertensive from diet-induced obesity. Int J Obes (Lond). 2007;31:1652-1659. https://doi.org/10.1038/sj.ijo.0803426
  9. Wang Y, Song Y, Suo M, Jin X, Tian G. Telmisartan prevents highfat diet-induced hypertension and decreases perirenal fat in rats. J Biomed Res. 2012;26:219-225.
  10. Wirth A, Benyo Z, Lukasova M, Leutgeb B, Wettschureck N, Gorbey S, Orsy P, Horvath B, Maser-Gluth C, Greiner E, Lemmer B, Schutz G, Gutkind JS, Offermanns S. G12-G13-LARG-mediated signaling in vascular smooth muscle is required for salt-induced hypertension. Nat Med. 2008;14:64-68. https://doi.org/10.1038/nm1666
  11. Brooks DP, Ruffolo RR Jr. Pharmacological mechanism of angiotensin II receptor antagonists: implications for the treatment of elevated systolic blood pressure. J Hypertens Suppl. 1999;17:S27-32. https://doi.org/10.1097/00004872-199917010-00005
  12. Hall JE, do Carmo JM, da Silva AA, Wang Z, Hall ME. Obesityinduced hypertension: interaction of neurohumoral and renal mechanisms. Circ Res. 2015;116:991-1006. https://doi.org/10.1161/CIRCRESAHA.116.305697
  13. Kadakol A, Malek V, Goru SK, Pandey A, Bagal S, Gaikwad AB. Esculetin attenuates alterations in Ang II and acetylcholine mediated vascular reactivity associated with hyperinsulinemia and hyperglycemia. Biochem Biophys Res Commun. 2015;461:342-347. https://doi.org/10.1016/j.bbrc.2015.04.036
  14. Zhang X, Yan SM, Zheng HL, Hu DH, Zhang YT, Guan QH, Ding QL. A mechanism underlying hypertensive occurrence in the metabolic syndrome: cooperative effect of oxidative stress and calcium accumulation in vascular smooth muscle cells. Horm Metab Res. 2014;46:126-132.
  15. Savineau JP, Marthan R. Modulation of the calcium sensitivity of the smooth muscle contractile apparatus: molecular mechanisms, pharmacological and pathophysiological implications. Fundam Clin Pharmacol. 1997;11:289-299. https://doi.org/10.1111/j.1472-8206.1997.tb00841.x
  16. Somlyo AP, Somlyo AV. $Ca^{2+}$ sensitivity of smooth muscle and nonmuscle myosin II: modulated by G proteins, kinases, and myosin phosphatase. Physiol Rev. 2003;83:1325-1358. https://doi.org/10.1152/physrev.00023.2003
  17. Margolis M, Perez O Jr, Martinez M, Santander AM, Mendez AJ, Nadji M, Nayer A, Bhattacharya S, Torroella-Kouri M. Phospholipid makeup of the breast adipose tissue is impacted by obesity and mammary cancer in the mouse: Results of a pilot study. Biochimie. 2015;108:133-139. https://doi.org/10.1016/j.biochi.2014.11.009
  18. Kim JI, Jung SW, Yang E, Park KM, Eto M, Kim IK. Heat shock augments angiotensin II-induced vascular contraction through increased production of reactive oxygen species. Biochem Biophys Res Commun. 2010;399:452-457. https://doi.org/10.1016/j.bbrc.2010.07.115
  19. Bray GA. Risks of obesity. Endocrinol Metab Clin North Am. 2003;32:787-804. https://doi.org/10.1016/S0889-8529(03)00067-7
  20. Soltani Z, Washco V, Morse S, Reisin E. The impacts of obesity on the cardiovascular and renal systems: cascade of events and therapeutic approaches. Curr Hypertens Rep. 2015;17:7. https://doi.org/10.1007/s11906-014-0520-2
  21. Dharmarajan TS, Dharmarajan L. Tolerability of Antihypertensive Medications in Older Adults. Drugs Aging. 2015;32:773-796. https://doi.org/10.1007/s40266-015-0296-3
  22. Abraham HM, White CM, White WB. The comparative efficacy and safety of the angiotensin receptor blockers in the management of hypertension and other cardiovascular diseases. Drug Saf. 2015; 38:33-54. https://doi.org/10.1007/s40264-014-0239-7
  23. Curb JD, Schneider K, Taylor JO, Maxwell M, Shulman N. Antihypertensive drug side effects in the Hypertension Detection and Follow-up Program. Hypertension. 1988;11:II51-55.
  24. Massiera F, Bloch-Faure M, Ceiler D, Murakami K, Fukamizu A, Gasc JM, Quignard-Boulange A, Negrel R, Ailhaud G, Seydoux J, Meneton P, Teboul M. Adipose angiotensinogen is involved in adipose tissue growth and blood pressure regulation. FASEB J. 2001;15:2727-2729. https://doi.org/10.1096/fj.01-0457fje
  25. Cooper R, McFarlane-Anderson N, Bennett FI, Wilks R, Puras A, Tewksbury D, Ward R, Forrester T. ACE, angiotensinogen and obesity: a potential pathway leading to hypertension. J Hum Hypertens. 1997;11:107-111. https://doi.org/10.1038/sj.jhh.1000391
  26. Li H, Li M, Liu P, Wang Y, Zhang H, Li H, Yang S, Song Y, Yin Y, Gao L, Cheng S, Cai J, Tian G. Telmisartan ameliorates nephropathy in metabolic syndrome by reducing leptin release from perirenal adipose tissue. Hypertension. 2016;68:478-490. https://doi.org/10.1161/HYPERTENSIONAHA.116.07008
  27. Maneesai P, Bunbupha S, Kukongviriyapan U, Prachaney P, Tangsucharit P, Kukongviriyapan V, Pakdeechote P. Asiatic acid attenuates renin-angiotensin system activation and improves vascular function in high-carbohydrate, high-fat diet fed rats. BMC Complement Altern Med. 2016;16:123. https://doi.org/10.1186/s12906-016-1100-6
  28. Yiannikouris F, Wang Y, Shoemaker R, Larian N, Thompson J, English VL, Charnigo R, Su W, Gong M, Cassis LA. Deficiency of angiotensinogen in hepatocytes markedly decreases blood pressure in lean and obese male mice. Hypertension. 2015;66:836-842. https://doi.org/10.1161/HYPERTENSIONAHA.115.06040
  29. Kosaka S, Pelisch N, Rahman M, Nakano D, Hitomi H, Kobori H, Fukuoka N, Kobara H, Mori H, Masaki T, Cervenka L, Matsumura Y, Houchi H, Nishiyama A. Effects of angiotensin II $AT_1$-receptor blockade on high fat diet-induced vascular oxidative stress and endothelial dysfunction in Dahl salt-sensitive rats. J Pharmacol Sci. 2013;121:95-102. https://doi.org/10.1254/jphs.12169FP
  30. Viswanad B, Srinivasan K, Kaul CL, Ramarao P. Effect of tempol on altered angiotensin II and acetylcholine-mediated vascular responses in thoracic aorta isolated from rats with insulin resistance. Pharmacol Res . 2006;53:209-215. https://doi.org/10.1016/j.phrs.2005.11.002
  31. Wynne BM, Chiao CW, Webb RC. Vascular smooth muscle cell signaling mechanisms for contraction to angiotensin II and endothelin-1. J Am Soc Hypertens. 2009;3:84-95. https://doi.org/10.1016/j.jash.2008.09.002
  32. Walsh MP, Susnjar M, Deng J, Sutherland C, Kiss E, Wilson DP. Phosphorylation of the protein phosphatase type 1 inhibitor protein CPI-17 by protein kinase C. Methods Mol Biol. 2007;365:209-223.
  33. Kobayashi T, Matsumoto T, Kamata K. The PI3-K/Akt pathway: roles related to alterations in vasomotor responses in diabetic models. J Smooth Muscle Res. 2005;41:283-302. https://doi.org/10.1540/jsmr.41.283

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