Journal of the Korea Academia-Industrial cooperation Society (한국산학기술학회논문지)

The Korea Academia-Industrial cooperation Society (한국산학기술학회)
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The Study of Physiological and Antimicrobial Activities on the Citrus Junos Extracts with its Textures and Skin

유자과즙 추출물의 생리활성 및 항균활성에 관한 연구

  • Lee, Song Hee (Interdisciplinary Program of Perfume and Cosmetics, Graduate School of Chonnam National University) ;
  • Lee, Moo Sung (Faculty of Applied Chemical Engineering, Chonnam National University)
  • 이송희 (전남대학교 대학원 향장품학협동과정) ;
  • 이무성 (전남대학교 응용화학공학부)
  • Received : 2017.03.16
  • Accepted : 2017.04.07
  • Published : 2017.04.30
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Abstract

This study analyzed the antioxidant activities and antibacterial effects of extracts from the fruit of Citrus junos. Theextracts were obtained in both 70% ethanol solution and distilled water at a distillation temperature of $50^{\circ}C$.Three experiments were carried out between November 2016 and March 2017. The averages and the standard deviations of the results were measured. The total polyphenol and tannin contents of the ethanol extracts were $7.8{\pm}0.02mgGAE/g$ and $9.9{\pm}0.01mgTAE/g$, respectively, which were higher than the concentrations in the water extracts. Furthermore, the ethanol extracts scavenged $46.1{\pm}4.76%$ of DPPH radicals and $37.1{\pm}1.23%$ of ABTS radicals. The scavenging capability of the ethanol extracts was also higher than that of the water extracts. However, the scavenging capability of both types of extracts depended on their concentrations.All the extracts showed active antibiosis effects against every bacteria tested except for C. albicans. E. coli at 25 mg/disc showed antibiosis with $18{\pm}1.73mm$ for the water extracts, while S. epidermidis and S. aureus showed antibiosis with $17{\pm}4.36mm$ and $19{\pm}2.86mm$ for the ethanol extracts, respectively. This antibiosis rate is considerably higher. The results suggest that fruit extract from Citrus junos could be useful as a primary material for beauty or skin-related products such as soaps, shampoos, and scalp enhancers.

본 연구는 유자의 과즙을 $50^{\circ}C$ 의 열수 추출물과 70%에탄올 추출물을 이용하여 항산화 효능과 항균효과를 2016년 11월부터 2017년 3월까지 실험하여 분석하였다. 실험결과는 모두 3회 반복 측정하여 평균값(mean)과 표준편차(standard deviation, SD)로 나타내었다. 연구 결과 총 polyphenol과 Tannin함량은 70%에탄올 추출물에서 $7.8{\pm}0.02mgGAE/g$, $9.9{\pm}0.01mgTAE/g$으로 열수 추출물보다 높은 함유량을 나타냈으며, DPPH radical의 소거능과 ABTS radical 소거능역시 70% 에탄올 추출물에서 농도가 10000 ppm일 때 각각 $46.1{\pm}4.76%$, $37.1{\pm}1.23%$로 열수 추출물보다 높게 나타났다. 항균효과에서는 C. albicans를 제외하고 모든 균에서 항균활성을 나타내었으며, 농도 25 mg/disc에서 E. coli는 $50^{\circ}C$ 열수추출물에서 $18{\pm}1.73mm$, S. epidermidis와 S. aureus는 70% 에탄올 추출물 에서 $17{\pm}4.36mm$, $19{\pm}2.86mm$로 열수추출물과 에탄올 추출물 모두 높은 항균력이 있음을 확인 하였다. 이와 같은 결과로 보았을 때 유자과즙추출물을 이용하여 비누, 샴푸 또는 두피개선제등 미용 또는 피부 관련 향장소재로서 활용할 수 있는 기초자료로서 충분한 가치가 있을 것으로 판단된다.

Keywords

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