Geomechanics and Engineering

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Measuring elastic modulus of bacterial biofilms in a liquid phase using atomic force microscopy

  • Kim, Yong-Min (Department of Civil and Environmental Engineering, Korean Advanced Institute for Science and Technology) ;
  • Kwon, Tae-Hyuk (Department of Civil and Environmental Engineering, Korean Advanced Institute for Science and Technology) ;
  • Kim, Seungchul (Department of Optics and Mechatronics Engineering, College of Nanoscience & Nanotechnology, Pusan National University)
  • Received : 2016.10.21
  • Accepted : 2017.02.15
  • Published : 2017.05.25
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Abstract

With the increasing interest in using bacterial biofilms in geo-engineering practices, such as soil improvement, sealing leakage in earth structures, and hydraulic barrier installation, understanding of the contribution of bacterial biofilm formation to mechanical and hydraulic behavior of soils is important. While mechanical properties of soft gel-like biofilms need to be identified for appropriate modeling and prediction of behaviors of biofilm-associated soils, elastic properties of biofilms remain poorly understood. Therefore, this study investigated the microscale Young's modulus of biofilms produced by Shewanella oneidensis MR-1 in a liquid phase. The indentation test was performed on a biofilm sample using the atomic force microscopy (AFM) with a spherical indentor, and the force-indentation responses were obtained during approach and retraction traces. Young's modulus of biofilms was estimated to be ~33-38 kPa from these force-indentation curves and Hertzian contact theory. It appears that the AFM indentation result captures the microscale local characteristics of biofilms and its stiffness is relatively large compared to the other methods, including rheometer and hydrodynamic shear tests, which reflect the average macro-scale behaviors. While modeling of mechanical behaviors of biofilm-associated soils requires the properties of each component, the obtained results provide information on the mechanical properties of biofilms that can be considered as cementing, gluing, or filling materials in soils.

Keywords

Acknowledgement

Supported by : National Research Foundation of Korea(NRF), Korea Agency for Infrastructure Technology Advancement (KAIA)

References

  1. Aggarwal, S. and Hozalski, R.M. (2010), "Determination of biofilm mechanical properties from tensile tests performed using a micro-cantilever method", Biofouling, 26(4), 479-486. https://doi.org/10.1080/08927011003793080
  2. Baniasadi, M., Xu, Z., Gandee, L., Du, Y., Lu, H., Zimmern P. and Minary-Jolandan, M. (2014), "Nanoindentation of Pseudomonas aeruginosa bacterial biofilm using atomic force microscopy", Mater. Res. Express, 1(4), 045411. https://doi.org/10.1088/2053-1591/1/4/045411
  3. Cense, A.W., Peeter, E.A.G., Gottenbos, B., Baaijens, F.P.T., Nuijs, A.M. and Dongen, M.E.H. (2006), "Mechanical properties and failure of Streptococcus mutans biofilms, studies using a microindentation device", J. Microbiol. Methods, 67(3), 463-472. https://doi.org/10.1016/j.mimet.2006.04.023
  4. Chang, I. and Cho, G.-C. (2012), "Strengthening of Korean residual soil with ${\beta}$-1,3/1,6-glucan biopolymer", Constr. Build. Mater., 30, 30-35. https://doi.org/10.1016/j.conbuildmat.2011.11.030
  5. Chang, I. and Cho, G.C. (2014), "Geotechnical behavior of a ${\beta}$-1,3/1,6-glucan biopolymer-treated residual soil", Geomech. Eng., Int. J., 7(6), 633-647. https://doi.org/10.12989/gae.2014.7.6.633
  6. Chang, I., Im, J., Prasidhi, A.K, and Cho, G.C. (2015), "Effects of Xanthan gum biopolymer on soil strengthening", Constr. Build. Mater., 74, 65-72. https://doi.org/10.1016/j.conbuildmat.2014.10.026
  7. Chang, I., Im, J. and Cho, G.C. (2016), "Geotechnical engineering behaviors of gellan gum biopolymer treated sand", Can. Geotech. J., 53(10), 1658-1670. https://doi.org/10.1139/cgj-2015-0475
  8. Dennis, M. and Turner, J. (1998), "Hydraulic conductivity of compacted soil treated with biofilm", J. Geotech. Geoenviron. Eng., 124(2), 120-127. https://doi.org/10.1061/(ASCE)1090-0241(1998)124:2(120)
  9. Donlan, R.M. (2002), "Biofilms: Microbial Life on Surfaces", Emerg. Infect. Diseases, 8(9), 881-890. https://doi.org/10.3201/eid0809.020063
  10. Huang, Q., Wu, H., Cai, P., Fein, J.B. and Chen, W. (2015), "Atomic force microscopy measurements of bacterial adhesion onto clay sized particles", Scientific Reports, 5, 16587. https://doi.org/10.1038/srep16587
  11. Hertz, H. (1882), "Uber die Beruhrung fester elastischer Korper", Journal fur die reine und angewandte Mathematik, 92, 156-171.
  12. Ivanov, V. and Chu, J. (2008), "Applications of microorganisms to geotechnical engineering for bioclogging and biocementation of soil in situ", Rev. Environ. Sci. Bio/Technology, 7(2), 139-153. https://doi.org/10.1007/s11157-007-9126-3
  13. Korstgens, V., Flemming, H., Wingerder, J. and Borchard, W. (2001), "Uniaxial compression measurement device for investigation of the mechanical stability of biofilms", J. Microbiol. Methods, 46(1), 9-17. https://doi.org/10.1016/S0167-7012(01)00248-2
  14. Kundukad, B., Sevious, T., Liang, Y., Rice, S.A., Kjelleberg, S. and Doyle, P.S. (2016), "Mechanical properties of the superficial biofilm layer determine the architecture of biofilms", Soft Matter, 12, 5718-5726. https://doi.org/10.1039/C6SM00687F
  15. Laspidou, C. and Aravas, N. (2007), "Variation in the mechanical properties of a porous multi-phase biofilm under compression due to void closure", Water Sci. Technol., 55(8-9), 447-453. https://doi.org/10.2166/wst.2007.289
  16. Lau, P.C.Y., Dutcher, J.R., Beveridge, T.J. and Lam, J.S. (2009), "Absolute Quantitation of bacterial biofilm adhesion and viscoelasticity by microbead force spectroscopy", Biophys. J., 96(7), 2935-2948. https://doi.org/10.1016/j.bpj.2008.12.3943
  17. Lewandowski, Z. and Beyenal, H. (2013), Fundamentals of Biofilm Research, (2nd Ed.), CRC press, Taylor & Francisco Group, New York, NY, USA.
  18. Sidik, W.S., Canakci, H., Kilic, I.H. and Celic, F. (2014), "Applicability of biocementation for organic soil and its effect on permeability", Geomech. Eng., Int. J., 7(6), 649-663. https://doi.org/10.12989/gae.2014.7.6.649
  19. Stoodley, P., Lewandowski, Z., Boyle, J.D. and Lappin-Scott, H.M. (1999), "Structural deformation of bacterial biofilms caused by short-term fluctuations in fluid shear: An in situ investigation of biofilm rheology", Biotech. Bioeng., 65(1), 83-92. https://doi.org/10.1002/(SICI)1097-0290(19991005)65:1<83::AID-BIT10>3.0.CO;2-B
  20. Stoodley, P., Cargo, R., Rupp, C.J., Wilson, S. and Klapper, I. (2002), "Biofilm material properties as related to shear-induced deformation and detachment phenomena", J. Indust. Microbiol. Biotechnol., 29(6), 361-367. https://doi.org/10.1038/sj.jim.7000282
  21. Thullner, M., Zeyer, J. and Kinzelbach, W. (2002), "Influence of microbial growth on hydraulic properties of pore networks", Transp. Porous Media, 49(1), 99-122. https://doi.org/10.1023/A:1016030112089
  22. Touhami, A., Nysten, B. and Dufrene, Y.F. (2003), "Nanoscale mapping of the elasticity of microbial cells by atomic force microscopy, Langmuir, 19(11), 4539-4543. https://doi.org/10.1021/la034136x
  23. Venkateswaran, K., Moser, D.P., Dollhopf, M.E., Lies, D.P., Saffarini, D.A., MacGregor, B.J., Ringelberg, D.B., White, D.C., Nishijima, M., Sano, Hiroshi., Burghardt, J., Stackebrandt, E. and Nealson, K.H. (1999), "Polyphasic taxonomy of the genus Shewanella and description of Shewanella oneidensis sp", Int. J. System. Bacteriol., 49, 705-724 https://doi.org/10.1099/00207713-49-2-705
  24. Yasodian, S.E., Dutta, R.K., Mathew, L., Anima, T.M. and Seena, S.B. (2012), "Effect of microorganism on engineering properties of cohesive soils", Geomech. Eng., Int. J., 4(2), 135-150. https://doi.org/10.12989/gae.2012.4.2.135

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