Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Investigation of Antioxidant Activity of Houttuyniae Herba and its Effect on 5α-reductase Gene Expression in Dermal Papilla Cells

어성초(魚腥草)의 항산화 효능 확인 및 모유두 세포의 5α-reductase 유전자 발현에 미치는 영향

  • Cho, Nam Joon (Department of Biochemistry, Chungnam National University) ;
  • Lee, Byeong Kwon (Department of Biochemistry, Chungnam National University) ;
  • Lee, Woong Hee (Institute of Biotechnology, Chungnam National University) ;
  • Kim, Kee Kwang (Department of Biochemistry, Chungnam National University) ;
  • Han, Hyo Sang (Department of Health Administration, College of Social Sciences, Joongbu University)
  • Received : 2017.07.28
  • Accepted : 2017.12.07
  • Published : 2017.12.25
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Abstract

Houttuyniae Herba is widely used as a cosmetic for enhancing hair growth, and study on promoting mouse hair growth has also been reported. However, studies on the effects of the Houttuyniae Herba on dermal papilla (DP) cells, which play an important role in hair growth, are not well known. For this reason, we studied the effect of Houttuyniae Herba on DP cells. The strong antioxidant activity of Houttuyniae Herba was confirmed by ABTS assay. In the MTS assay, cell viability was reduced to 94.5% in DP cells by treatment of 2 mg/ml concentration of Houttuyniae Herb and cytotoxicity was not observed at 1 mg/ml concentration. The mRNA expression levels of Bone morphogenetic pretein (BMP6), fibroblast growth factor 7 (FGF7), FGF10, and ${\beta}$-galactosidase genes, which are involved in hair growth cycle and hair loss induction, were measured by quantitative RT-PCR after Houttuyniae Herbtreatment. Houttuyniae Herb did not significantly affect mRNA expression of BMP6, FGF7, FGF10, and ${\beta}$-catenin, which are important factors for regulating the hair cycle, including type 1 $5{\alpha}$-reductase. However, mRNA expression of type 2 $5{\alpha}$-reductase, the major cause of male hair loss, was significantly reduced to 56.1% by treatment of Houttuyniae Herbtreatment. Taken together, these results suggest that the Houttuyniae Herbtreatment can help to treat lair loss through removing free radicals and suppression of the expression level of type 2 $5{\alpha}$-reductase in DP cells.

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