Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Effects of Gami-Shinkiwhan on the Reproductive Function of Male Aged Mice

가미신기환(加味腎氣丸)이 수컷 노화 생쥐의 생식기능에 미치는 영향

  • Park, Sun Young (Department of Physiology, College of Korean Medicine, Semyung University) ;
  • Ahn, Sang Hyun (Department of Anatomy, College of Korean Medicine, Semyung University) ;
  • Kim, Ho Hyun (Department of Physiology, College of Korean Medicine, Semyung University)
  • 박선영 (세명대학교 한의과대학 생리학교실) ;
  • 안상현 (세명대학교 한의과대학 해부학교실) ;
  • 김호현 (세명대학교 한의과대학 생리학교실)
  • Received : 2017.10.13
  • Accepted : 2017.12.14
  • Published : 2017.12.25
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Abstract

This study was performed using histochemical and immunohistochemical methods to investigate the effect of Gami-Shinkiwhan(GS) on reproductive function of male aged mice. 8-weeks-old ICR mice were used as control group, without any treatment, and 15-month-old ICR mice were used as aging elicited group(AE) and Gami-Shinkiwhan treatment group(GS). AE group didn't restrict diets and drinking for 6 months without any treatment. GS was administered 0.56g/kg/day for 6 months. Compared with AE group, the cell division of sertoli cells, spermatids, and spermatogonial cells was increased and the apoptosis of sertoli cells was decreased on GS group. Androgen receptor positive reaction and $17{\beta}$-HSD positive reaction were significantly increased in the GS group compared to AE group. In addition, the DJ-1 positive reaction was significantly increased and the HDAC3 positive response was significantly decreased in the GS group compared with AE group. Based on the above results, GS prevented the apoptosis of sertoli cells in the tubules, and increased the production of sertoli cells, spermatozoa and testosterone. Based on this, it is thought that it improves male reproductive dysfunction caused by late-onset hypogonadism.

Keywords

References

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