Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Investigation of the Antioxidant Effect of Angelicae Radix from Korea, China and Japan

참당귀, 중국당귀, 일당귀의 차등적 항산화 효능 연구

  • Cho, Nam Joon (Department of Biochemistry, Chungnam National University) ;
  • Lee, Woong Hee (Institute of Biotechnology, Chungnam National University) ;
  • Kim, Kee Kwang (Department of Biochemistry, Chungnam National University) ;
  • Han, Hyo Sang (Department of Health Administration, College of Social Sciences, Joongbu University)
  • Received : 2017.03.15
  • Accepted : 2017.04.22
  • Published : 2017.06.25
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Abstract

The purpose of the present study is a comparison of the antioxidant effects of Angelica gigas Korea (AG), Angelica sinensis of China (AS), and Angelica acutiloba of Japan (AA), and comparison of the effects of AG, AS and AA on tight-junction related genes in human keratinocyte HaCaT cells. All species showed a strong antioxidant effect, and AA was higher than AG and AS in antioxidant effects. The cytotoxicity was confirmed to be higher in AS than AG and AA at a concentration of $1,600{\mu}g/ml$ using the MTS assay in HaCaT cells. We analyzed the effects of AG, AS, and AA on mRNA expression levels of various tight-junction related genes in HaCaT cells. We found that no obvious changes in expression of Claudin 1, 3, 4, 6, 7, 8, Occludin, JAM-A, ZO-1, ZO-2, and tricellulin by treatment of all species, suggesting that there is less possibility of side effects and skin moisturizing effects due to changes in tight-junction gene expression. Our results suggest that AG, AS, and AA are thought to be effective in reducing the oxidative stress of the skin and preventing the aging of the skin.

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References

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