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참당귀, 중국당귀, 일당귀의 차등적 항산화 효능 연구

Investigation of the Antioxidant Effect of Angelicae Radix from Korea, China and Japan

  • Cho, Nam Joon (Department of Biochemistry, Chungnam National University) ;
  • Lee, Woong Hee (Institute of Biotechnology, Chungnam National University) ;
  • Kim, Kee Kwang (Department of Biochemistry, Chungnam National University) ;
  • Han, Hyo Sang (Department of Health Administration, College of Social Sciences, Joongbu University)
  • 투고 : 2017.03.15
  • 심사 : 2017.04.22
  • 발행 : 2017.06.25

초록

The purpose of the present study is a comparison of the antioxidant effects of Angelica gigas Korea (AG), Angelica sinensis of China (AS), and Angelica acutiloba of Japan (AA), and comparison of the effects of AG, AS and AA on tight-junction related genes in human keratinocyte HaCaT cells. All species showed a strong antioxidant effect, and AA was higher than AG and AS in antioxidant effects. The cytotoxicity was confirmed to be higher in AS than AG and AA at a concentration of $1,600{\mu}g/ml$ using the MTS assay in HaCaT cells. We analyzed the effects of AG, AS, and AA on mRNA expression levels of various tight-junction related genes in HaCaT cells. We found that no obvious changes in expression of Claudin 1, 3, 4, 6, 7, 8, Occludin, JAM-A, ZO-1, ZO-2, and tricellulin by treatment of all species, suggesting that there is less possibility of side effects and skin moisturizing effects due to changes in tight-junction gene expression. Our results suggest that AG, AS, and AA are thought to be effective in reducing the oxidative stress of the skin and preventing the aging of the skin.

키워드

참고문헌

  1. Wu, B. Shennongbencaojing. Beijing:Renminweishengchu banshe. 1982:64.
  2. Kim IR, Kim HC, Kuk YB, Park SJ, Park YK, Park JH, Seo BI, Seo YB, Shin MK, Lee YJ, Lee YC, Lee JH, Leem KH, Cho SI, Chung JK, Joo YS, Choi HY. Boncho-Hak. Seoul:Young-Lim Press. 2007:629-31.
  3. Ahn KS, Sim WS, Kim IH. Decursin a cytotoxic agent and protein kinase c activator from the root of angelica gigas. Planta Med. 1995;62:7-9.
  4. Kim CM, Shin MG, Lee GS, Ahn DK. Wanyeok Jungyakdaesajeon. Seoul:Jeongdam. 1998;3:1159-68.
  5. Oh HS. Comparative studies of the angiogeni activity of extract of angelica gigas, A. sinensis and A. acutiloba. [Ph.D. dissertation]. Kyunghee University; 2001.
  6. Dakaki, K. Whahanyakmoolhak. Tokyo:Namsandang. 1982:293-7.
  7. Tsuruta D, Green KJ, Getsios S, Jones JC. The barrier function of skin: how to keep a tight lid on water loss. Trends Cell Biol. 2002;112(8):355-7.
  8. Masato M, Fumiyoshi Y, Akemi I, Keiko Y, Chikaako K, Shinji F, Eiichiro U, Yohichi M, Kohichi T, Hirokazu Y, Mituru H, Hajime I, Masahito I, Masaru O, Gen K, Taroh K, Junji T, Kiyofumi Y. Defective stratum corneum and early neonatal death in mice lacking the gene for transglutaminase 1 (keratinocyte transglutaminase). Proc Natl. Acad. Sci USA. 1998;95(3):1044-9. https://doi.org/10.1073/pnas.95.3.1044
  9. Kang MS, Ha HY, Kim HT. An Experimental Study on the Effect of Angelica acutiloba Ethanol Extract on Hyaluronic Acid Synthesis. J Korean Med Ophthalmol Otolaryngol Dermatol. 2015;28(1):32-40. https://doi.org/10.6114/jkood.2015.28.1.032
  10. Kim DH, Kim HM, Ryu JH, Eom JY, Kim SC, Yang JH, Cho MK, Lim JP, Hong SH. Hanbangyakrihak. Seoul:Shinilbukseu. 2007:337-43.
  11. Kang SA, Han JA, Jang KH, Choue RW. DPPH Radical Scavenger Activity and Antioxidant Effects of Cham - Dang - Gui(Angelica gigas). J Korean Soc Food Sci Nutr. 2004;33(7):1112-8. https://doi.org/10.3746/jkfn.2004.33.7.1112
  12. Choo MH, Choi HS, Seo YN, Lee MY. Effects of n-Hexane Fraction of Angelica acutiloba on Antioxidative System and Lipid peroxidation in Ethanol-Induced Hepatotoxicity of rats. Korean Journal of Food P reservation. 2004;11(3):364-72.
  13. Yu HS, Park CH, Park CG, Kim YG, Park HW, Seong NS. Growth Characteristics and Yield of the Three Species of Genus Angelica. Korean J Medicinal Crop Sci.. 2004;12(1):43-6.
  14. Lee MY, Im SH, Ju YS, Han KS, Jeong GJ, Ahn DK, Kang HC, Ko BS. Discrimation of the three Angelica species using the RADPs and Internal Root Structure. Korean J Medicinal Crop Sci. 2000;8(3):243-9.
  15. Noh BS, Oh SY, Kim SJ. Pattern Analysis of Volatile Components for Domestic and Imported Angelica gigas Nakai Using GC Based on SAW Sensor. Korean J Food Sci. Technol. 2003;35(1):144-8.
  16. Bang KH, Yu HS, Koo DH, Cho JH, Park HW, Seong NS, Park SI, Kim HS. Selection of RAPD marker to discriminate the bolting-resistant varieties and commercial dried medicinal materials of Angelica species. Korean J Medicinal Crop Sci. 2002;10(1):46-50.
  17. Cho MG, Bang JK, Chae YA. Comparison of Volatile Compounds in Plant Parts of Angelica gigas Nakai and A. acutiloba Kitagawa. Korean J Medicinal Crop Sci. 2003;11(5):352-7.
  18. Sung JS, Bang KH, Park CH, Park CG, Yu HS, Park HW, Seong N.S Discrimination of Angelicae Radix Based on Anatomical Characters. Korean J Medicinal Crop Sci. 2004;12(1):67-72.
  19. Inn MK, Kang CK, Jung NI, Chang WH, Lai JH, Yook CS. Essential Oils in the Rhizoma of Ligusticum sinensis and Ligusticum Chuanxing. Bull. K.H. Pharma. Sci. 2000;28:33-43.
  20. Ham MS, Kim SS, Hong JS, Lee JH, Chung EK, Park YS, Lee HY. Screening and Comparison of Active Substances of Angelica gigas Nakai Produced in Kangwon and Angelica acutiloba Kitogawa Produced in Japan. Kor. J Appl. Microbiol. Biotechol. 1996;24(5):624-9.
  21. Kang SA, Jang KH, Lee JE, Ahn DK, Park SK. Differences of Hematopoietic Effects of Angelica gigas, A. sinensis and A. acutiloba Extract on Cyclophosphamide-induced Anemic Rats. Korean J Food Sci. Technol. 2003;35(6):1204-8.
  22. Oh HS, Kim HH, Ahn DK, Choi HY. Comparative studies on the angiogenic activity of water extract of Angelica Gigantis Radix , Angelica Sinensis Radix and Angelica Radix. Kor. J Herbology. 2001;16(2):19-27.
  23. Song SH, Seo BI, Kim HG, Park JH. The Effects of Angelicae Gigantis Radix, Angelicae Acutilobae Radix and Angelicae Sinensis Radix Extract on Hydrocortisone Acetate-Induced Model of Blood Stasis. Kor. J Herbology. 2004;19(1):13-21.
  24. Bickers DR, Athar M. Oxidative Stress in the Pathogenesis of Skin Disease. Journal of Investigative Dermatology. 2006;126(12):2565-75. https://doi.org/10.1038/sj.jid.5700340
  25. Schneeberger EE, Lynch RD. The tight junction: a multifunctional complex. Am J Physiol Cell Physiol. 2004;286:C1213-28. https://doi.org/10.1152/ajpcell.00558.2003
  26. Soler AP, Miller RD, Laughlin KV, Carp NZ, Klurfeld DM, Mullin JM. Increased tight junctional permeability is associated with the development of colon cancer. Carcinogenesis. 1999;20:1425-31. https://doi.org/10.1093/carcin/20.8.1425
  27. De Benedetto A, Rafaels NM, McGirt LY, Ivanov AI, Georas SN, Cheadle C, Berger AE, Zhang K, Vidyasagar S, Yoshida T, Boguniewicz M, Hata T, Schneider LC, Hanifin JM, Gallo RL, Novak N, Weidinger S, Beaty TH, Leung DY, Barnes KC, Beck LA. Tight junction defects in patients with atopic dermatitis. Journal of Allergy and Clinical Immunology. 2011;127(3):773-86. https://doi.org/10.1016/j.jaci.2010.10.018
  28. Yoshida Y, Morita K, Mizoguchi A, Ide C, Miyachi Y. Altered expression of occludin and tight junction formation in psoriasis. Archives of Dermatological Research. 2001;293(5):239-44. https://doi.org/10.1007/s004030100221
  29. Peltonen S, Riehokainen J, Pummi K, Peltonen J. Tight junction components occludin, ZO-1, and Claudin-1, -4 and -5 in active and healing psoriasis. British Journal of Dermatology. 2007;156(3):466-72. https://doi.org/10.1111/j.1365-2133.2006.07642.x
  30. Hadj-Rabia S, Baala L, Vabres P, Hamel-Teillac D, Jacquemin E, Fabre M, Lyonnet S, Prost YD, Munnich A, Hadchouel M, Smahi A. Claudin-1 Gene Mutations in Neonatal Sclerosing Cholangitis Associated With Ichthyosis: A Tight Junction Disease. Gastroenterology. 2004;127(5):1386-90. https://doi.org/10.1053/j.gastro.2004.07.022
  31. Turksen K, Troy TC. Permeability barrier dysfunction in transgenic mice overexpressing Claudin 6. Development. 2002;129:1775-84.

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