Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Investigation of the Effect of Water Extract of Lithospermi Radix on the Expression of IL-1β, TNF-α and iNOS Genes in Raw 264.7 Cells

자초(紫草) 열수 추출물의 RAW 264.7 세포에서 IL-1β, TNF-α, iNOS 유전자 발현에 미치는 영향 연구

  • Cho, Nam Joon (Department of Biochemistry, Chungnam National University) ;
  • Choi, Young Ho (Department of Biochemistry, Chungnam National University) ;
  • Lee, Woong Hee (Institute of Biotechnology, Chungnam National University) ;
  • Kim, Kee Kwang (Department of Biochemistry, Chungnam National University) ;
  • Han, Hyo Sang (Department of Health Administration, College of Social Sciences, Joongbu University)
  • Received : 2017.05.02
  • Accepted : 2017.08.10
  • Published : 2017.08.25
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Abstract

Lithospermi Radix (LR) is known to have an anti-inflammatory effect. However, the mechanisms are not well known. In this study, LPS-induced mouse RAW 264.7 macrophage cells were treated with LR to investigate the time-dependent inflammation response of LR. RAW 264.7 cells were treated with various concentrations of LR for 24 hours, followed by MTS assay. Cell viability was increased at all experimental concentrations. The mRNA expression levels of $IL-1{\beta}$, $TNF-{\alpha}$ and iNOS were increased by treatment of RAW 264.7 cells with LR at a concentration of $200{\mu}g/ml$ for 6 hours and 24 hours. Treatment of LR with $200{\mu}g/ml$ concentration for 6 hours promoted mRNA expression levels of $IL-1{\beta}$, $TNF-{\alpha}$ and iNOS in LPS-induced RAW 264.7 cells. However, $IL-1{\beta}$, $TNF-{\alpha}$ and iNOS mRNA expression was suppressed by treatment of LR with $200{\mu}g/ml$ concentration for 24 hours in LPS-induced RAW 264.7 cells. These results suggest that the effect on inflammation of LR is promptly promoted and then to rapidly alleviate the inflammatory reaction. This study proposes that the time-dependent activities of herbal medicine is a very important factor in analyzing the anti-inflammatory effect of various herbal medicines including LR.

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References

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