Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Melanogenic Effect and the Mechanism of Epimedium koreanum Nakai

삼지구엽초의 멜라닌합성 촉진과 작용기전

  • Cha, Su Bin (Department of Herbal Resources, Professional Graduate School of Korean Medicine, Wonkwang University) ;
  • Kim, Dan Hee (Department of Herbal Resources, Professional Graduate School of Korean Medicine, Wonkwang University) ;
  • Mun, Yeun Ja (Department of Herbal Resources, Professional Graduate School of Korean Medicine, Wonkwang University) ;
  • Woo, Won Hong (Department of Anatomy, College of Korean Medicine, Wonkwang University)
  • 차수빈 (원광대학교 한의학전문대학원 한약자원개발학과) ;
  • 김단희 (원광대학교 한의학전문대학원 한약자원개발학과) ;
  • 문연자 (원광대학교 한의학전문대학원 한약자원개발학과) ;
  • 우원홍 (원광대학교 한의과대학 해부학교실)
  • Received : 2017.03.10
  • Accepted : 2017.08.24
  • Published : 2017.08.25
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Abstract

In this study, ethanol extract of Epimedium koreanum Nakai(EEKN) enhanced melanogenesis by inducing expression of tyrosinase and tyrosinase-related protein-1 (TRP-1). But EEKN did not increase the protein expression of tyrosinase-related protein 2 (TRP-2). Moreover, EEKN enhanced tyrosinase activity and melanin contents of B16F10 cells. EEKN raised the expression of CREB phosphorylation and microphthalmia-associated transcription factor (MITF) as a key transcription factor for tyrosinase expression regulating melanogenesis. And PKC inhibitor H89 supressed that EEKN induced tyrosinase activity, melanin contents, and expression of tyrosinase, TRP-1. These results suggest that melanogenesis-promoting effect of EEKN was correlated with regulation of tyrosinase and TRP-1 protein through cAMP/PKC pathway.

Keywords

References

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