Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Protective Effects of Prunella Vulgaris on Glomerular Injury in Streptozotocin-Induced Diabetic Rats

하고초 추출물의 streptozotocin 유발 당뇨 랫트 사구체 손상 개선 효과

  • Yoon, Jung Joo (Department of Phygiology, College of Korean Medicine, Wonkwang University) ;
  • Park, Ji Hun (Department of Phygiology, College of Korean Medicine, Wonkwang University) ;
  • Jeong, Da Hye (Department of Phygiology, College of Korean Medicine, Wonkwang University) ;
  • Han, Byung Hyuk (Department of Phygiology, College of Korean Medicine, Wonkwang University) ;
  • Choi, Eun Sik (Hanbang Body-fluid Research Center, Wonkwang University) ;
  • Lee, Yun Jung (Department of Phygiology, College of Korean Medicine, Wonkwang University) ;
  • Kang, Dae Gill (Department of Phygiology, College of Korean Medicine, Wonkwang University) ;
  • Lee, Ho Sub (Department of Phygiology, College of Korean Medicine, Wonkwang University)
  • 윤정주 (원광대학교 한의과대학 생리학교실) ;
  • 박지훈 (원광대학교 한의과대학 생리학교실) ;
  • 정다혜 (원광대학교 한의과대학 생리학교실) ;
  • 한병혁 (원광대학교 한의과대학 생리학교실) ;
  • 최은식 (원광대학교 한방체액조절연구센터) ;
  • 이윤정 (원광대학교 한의과대학 생리학교실) ;
  • 강대길 (원광대학교 한의과대학 생리학교실) ;
  • 이호섭 (원광대학교 한의과대학 생리학교실)
  • Received : 2017.08.16
  • Accepted : 2017.10.18
  • Published : 2017.10.25
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Abstract

Prunella vulgaris, well-known traditional medicinal plant, is used for the cure of abscess, scrofula, hypertension and urinary diseases. Diabetic nephropathy is the most common cause of end-stage renal disease. The pathological characteristics of diabetic nephropathy are glomerular and tubular basement membrane thickening. The aim of the present study was to evaluate the effect of Prunella vulgaris, on diabetic glomerular injury in streptozotocin-induced diabetes rats. Diabetes mellitus was induced by a single intraperitoneal injection of streptozotocin (STZ; 45 mg/kg) and confirmed by random glucose level higher than ${\leq}300mg/dL$. The experimental rats were divided into five groups: control group (Male SD rats), STZ group (Male SD rats injected STZ), Aminoguanidine group (Male SD rats injected STZ + AG 100 mg/kg/day), Low dose group (Male SD rats injected STZ + APV 100 mg/kg/day), High dose group (Male SD rats injected STZ + APV 300 mg/kg/day). AG or APVs were administered once a day for 8 weeks. Body weight and food/water intake were measured every four weeks. At the end of study, the kidneys were collected and cut into pieces for immunohistochemistry and western blot analysis. Our study showed that body weight and water/food intake were no significant differences between untreated STZ-induced diabetic rat and APV treated-STZ rat. However, phosphorylation of receptor-regulated Smads (Smad3) was significantly decreased in APV treated-STZ rat as compared with the diabetic group. In addition, APV was improved nephrin level in kidney tissue. Therefore, we suggest that APV has a protective effect against STZ-induced diabetic glomerular injury.

Keywords

References

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