Asian-Australasian Journal of Animal Sciences

  • 제30권7호
  • /
  • Pages.950-956
  • /
  • 2017
  • /
  • 1011-2367(pISSN)
  • /
  • 1976-5517(eISSN)
아세아태평양축산학회 (Asian Australasian Association of Animal Production Societies)
권호 표지
DOI QR코드

DOI QR Code

Development of a new lactic acid bacterial inoculant for fresh rice straw silage

  • Kim, Jong Geun (Graduate School of International Agricultural Technology and Institute of Green Bio Science & Technology, Seoul National University) ;
  • Ham, Jun Sang (Animal Products Research and Development Division, National Institute of Animal Science) ;
  • Li, Yu Wei (Graduate School of International Agricultural Technology and Institute of Green Bio Science & Technology, Seoul National University) ;
  • Park, Hyung Soo (Grassland and Forage Division, National Institute of Animal Science) ;
  • Huh, Chul-Sung (Graduate School of International Agricultural Technology and Institute of Green Bio Science & Technology, Seoul National University) ;
  • Park, Byung-Chul (Graduate School of International Agricultural Technology and Institute of Green Bio Science & Technology, Seoul National University)
  • 투고 : 2017.04.14
  • 심사 : 2017.05.18
  • 발행 : 2017.07.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Objective: Effects of newly isolated Lactobacillus plantarum on the fermentation and chemical composition of fresh rice straw silage was evaluated in this study. Methods: Lactic acid bacteria (LAB) from good crop silage were screened by growing them in MRS broth and a minimal medium with low carbohydrate content. Selected LAB (LAB 1821) were Gram-positive, rods, catalase negative, and were identified to be Lactobacillus plantarum based on their biochemical characteristics and a 16S rRNA analysis. Fresh rice straw was ensiled with two isolated LAB (1821 and 1841), two commercial inoculants (HM/F and P1132) and no additive as a control. Results: After 2 months of storage at ambient temperature, rice straw silages treated with additives were well-preserved, the pH values and butyric and acetic acid contents were lower, and the lactic acid content and lactic/acetic acid ratio were higher than those in the control (p<0.05). Acidity (pH) was lowest, and lactic acid highest, in 1821-treated silage (p<0.05). The $NH_3-N$ content decreased significantly in inoculant-treated silage (p<0.05) and the $NH_3-N$ content in 1821-treated silage was lowest among the treatments. The dry matter (DM) content of the control silage was lower than that of fresh rice straw (p<0.05), while that of the 1841- and p1174-inoculant-treated silages was significantly higher than that of HM/F-treated silage. Microbial additives did not have any significant (p>0.05) effect on acid detergent fiber or neutral detergent fiber contents. Crude protein (CP) content and in vitro DM digestibility (IVDMD) increased after inoculation of LAB 1821 (p<0.05). Conclusion: LAB 1821 increased the CP, IVDMD, lactic acid content and ratio of lactic acid to acetic acid in rice straw silage and decreased the pH, acetic acid, $NH_3-N$, and butyric acid contents. Therefore, adding LAB 1821 improved the fermentation quality and feed value of rice straw silage.

키워드

참고문헌

  1. MAFRA. The current situation of forage increase production and supplementation policy. Ministry of Agriculture, Food and Rural Affairs; 2015.
  2. Han YW, Anderson AW. The problem of rice straw waste a possible feed through fermentation. Econ Bot 1974;28:338-44. https://doi.org/10.1007/BF02861429
  3. Ralston AT, Kennick WH, Davidson TP, Rowe KE. Effect of prefinishing treatment upon finishing performance and carcass characteristics of beef cattle. J Anim Sci 1966;25:29-33. https://doi.org/10.2527/jas1966.25129x
  4. Cai Y. Development of lactic acid bacteria inoculant for whole crop rice silage in Japan. Proceeding of satellite symposium of XIIth AAAP Animal Science Congress 2006; Busan, Korea: Asian-Australas Assoc Anim Prod Soc; 2006. p. 85-9.
  5. Filya I, Muck RE, Contreras-Govea FE. Inoculant effects on alfalfa silage: fermentation products ans nutritive value. J Dairy Sci 2007;90: 5108-14. https://doi.org/10.3168/jds.2006-877
  6. Weinberg ZG, Muck RE. New trends and opportunities in the development and use of inoculants for silage. FEMS Microbiol Rev 1996;19: 53-68.
  7. AOAC. Official method of analysis. 15th ed. Washington, DC: AOAC International; 1990.
  8. Goering HK, Van Soest PJ. Forage fiber analysis. Washington, DC: Agric. Handbook 379, U.S. Gov. Print. Office, 1970.
  9. Tilley JMA, Terry RA. A two-stage technique for the in vitro digestion of forage crop. J Br Grassl Soc 1963;18:104-11. https://doi.org/10.1111/j.1365-2494.1963.tb00335.x
  10. Thomas TA. An automated procedure for the determination of soluble carbohydrates in herbage. J Sci Food Agric 1977;28:639-42. https://doi.org/10.1002/jsfa.2740280711
  11. Chaney AL, Marbach EP. Modified reagent for determination of urea and ammonia. Clin Biochem 1962;8:130.
  12. APHA. Standard methods for the examination of dairy products. Washington, DC: American Public Health Association; 1993.
  13. Pavlova S, Kilic A, Kilic C, et al. Genetic diversity of vaginal lactobacilli from women in different countries based on 16S rRNA gene sequences. J Appl Microbiol 2002;92:451-9. https://doi.org/10.1046/j.1365-2672.2002.01547.x
  14. SAS Institute Inc. SAS/STAT user guide. Cary, NC: SAS Institute Inc.;2002.
  15. Kaarel A, Kask S, Laht T, Paalma T. The effect of temoerature and pH on the growth of lactic acid bacteria: a pH-auxostat study. Int'l J Food Microbiol 2003;85:171-83. https://doi.org/10.1016/S0168-1605(02)00537-8
  16. Koc F, Coskuntuna L, Ozduven MJ, Coskuntuna A, Samli HE. The effects of temperature on the silage microbiology and aerobic stability of corn and vetch-grain silages. Acta Agric Scand Sec A Anim Sci 2009;59:239-46.
  17. Holland C, Kezar W, Kautz WP, et al. The pioneer forage manual-a nutritional guide. Des Moines, IA: Pioneer Hi-Bred International, Inc.; 1990.
  18. McDonald P, Henderson N, Heron S. The biochemistry of silage. 2nd ed., Marlow, UK: Chalcombe Publications; 1991. pp. 6-197.
  19. Jian L, Yixin S, Cai Y. Improvement of fermentation quality of rice straw silage by application of a bacterial inoculant and glucose. Asian-Australas J Anim Sci 2010;23:901-6. https://doi.org/10.5713/ajas.2010.90403
  20. Zhang Z, Scott S, Lukas W, Webb M. A greedy algorithm for aligning DNA sequences. J Comput Biol 2000;7:203-14. https://doi.org/10.1089/10665270050081478
  21. Muck RE. Factors influencing silage quality and their implications for management. J Dairy Sci 1988;55:454-60.
  22. Whiter AG, Kung L. The effect of a dry or liquid application of Lactobacillus plantarum MTD1 on the fermentation of alfalfa silage. J Dairy Sci 2001;84:2195-202. https://doi.org/10.3168/jds.S0022-0302(01)74666-8
  23. Wilkins M. Silage UK. 6th edition. Marlow, UK: Chalcombe Publications;1990.
  24. Haigh PM. Chemical composition and energy value of big bale silage in Englang 1984-1991. J Agric Eng Res 1995;60:211-6 https://doi.org/10.1006/jaer.1995.1015
  25. Kung L, Satter LD, Jones BA. Microbial inoculation of low moisture alfalfa silages. J Dairy Sci 1987;70:2069-77. https://doi.org/10.3168/jds.S0022-0302(87)80255-2
  26. Rooke JA, Maya FM, Arnold JA, Amstrong DG. The chemical composition and nutritive value of grass silage prepared with no additive or with the application of additives containing either Lactobacillus plantarum of formic acid. Grass Forage Sci 1988;43:87-95. https://doi.org/10.1111/j.1365-2494.1988.tb02144.x
  27. Zhang YG, Xin HS, Hue JL. Effects of treating whole-plant or chopped rice straw silage with different levels of lactic acid bacteria on sialge fermentation and nutritive value for lactating Holsteins. Asian-Australas J Anim Sci 2010;23:1601-7. https://doi.org/10.5713/ajas.2010.10082
  28. Keady TWJ, Steen RWJ. Effects of treating loe dry-matter grass with a bacterial inoculant on the intake and performance of beef cattle and studies on its mode of action. Grass Forage Sci 1994;49:438-46. https://doi.org/10.1111/j.1365-2494.1994.tb02021.x
  29. Mayne CS. An evaluation of an inoculant of Lactobacillus plantrum as an additive for grass silage for dairy cattle. Anim Prod 1990;51:1-3. https://doi.org/10.1017/S0003356100005109
  30. Aksu T, Baytok E, Bolat D. Effects of a bacterial silage inoculant on corn silage fermentation and nutrient digestibility. Small Rumin Res 2004;55:249-52. https://doi.org/10.1016/j.smallrumres.2003.12.012

피인용 문헌

  1. Effects of Lactic Acid Bacteria Inoculants on Fermentation of Low Moisture Fresh Rice Straw Silage at Different Storage Periods vol.39, pp.3, 2017, https://doi.org/10.5333/kgfs.2019.39.3.165
  2. Low-Carbohydrate Tolerant LAB Strains Identified from Rumen Fluid: Investigation of Probiotic Activity and Legume Silage Fermentation vol.8, pp.7, 2020, https://doi.org/10.3390/microorganisms8071044
  3. Improving sugarcane bagasse quality as ruminant feed with Lactobacillus, cellulase, and molasses vol.62, pp.5, 2020, https://doi.org/10.5187/jast.2020.62.5.648
  4. Changes in fermentation pattern and quality of Italian ryegrass (Lolium multiflorum Lam.) silage by wilting and inoculant treatments vol.34, pp.1, 2017, https://doi.org/10.5713/ajas.20.0336
  5. Manipulation of Rice Straw Silage Fermentation with Different Types of Lactic Acid Bacteria Inoculant Affects Rumen Microbial Fermentation Characteristics and Methane Production vol.8, pp.6, 2017, https://doi.org/10.3390/vetsci8060100