Journal of Forest and Environmental Science

Institute of Forest Science, kangwon National University (강원대학교 산림과학연구소)
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Rearing Temperature and Density Effects on the Number of Bacterial and Fungal Colonies in Metamorphosed Dybowski's Frogs (Rana dybowskii)

  • Kim, Jong-Sun (Department of Biology, Kangwon National University) ;
  • Choi, Woo-Jin (Department of Biology, Kangwon National University) ;
  • Park, Il-Kook (Department of Biology, Kangwon National University) ;
  • Koo, Kyo-Soung (Department of Biology, Kangwon National University) ;
  • Kang, Hui-Beom (Division of Science Education, Kangwon National University) ;
  • Kwon, Oh-Sung (Division of Science Education, Kangwon National University) ;
  • Lee, Seung-Hyeon (Division of Science Education, Kangwon National University) ;
  • Choi, Hye-Ji (Division of Science Education, Kangwon National University) ;
  • Lee, Jung-Hyun (Biological Resources Utilization Department, National Institute of Biological Resources) ;
  • Lee, Jin-Gu (Gyeonggi-do Agricultural Research & Extension Services) ;
  • Park, Dae-Sik (Division of Science Education, Kangwon National University)
  • Received : 2017.12.18
  • Accepted : 2018.02.07
  • Published : 2018.02.28
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Abstract

To know if small changes in rearing water temperature and density affect the number of bacterial and fungal colonies in metamorphosed frogs, Dybowski's frog tadpoles were reared from Gosner 25-26 stages at either low ($1^{\circ}C$ low to ambient water temperature), ambient, or high ($1^{\circ}C$ high) water temperature (each 15 tadpoles in 20 L water) condition and at either low (10 tadpoles/20 L water), medium (20 tadpoles), or high (30 tadpoles) density condition. Immediately after metamorphosis, we sampled bacteria and fungi from skin, liver, and heart of six metamorphosed frogs, randomly selected for each treatment group. After separate incubation of bacteria and fungi on 3M Petrifilm plates, we counted the number of bacterial and fungal colonies appeared on the plates and compared the numbers among the temperature and density treatment groups. For temperature treatment, high-temperature group had fewer bacterial colonies, while low-temperature group had more fungal colonies than the other two groups. For density treatment, low-density group had fewer bacterial colonies than the other two groups, but the number of fungal colonies were not different among the groups. Our results suggest that small increased rearing water temperature and lowered rearing density could potentially reduce pathogens in farming frogs.

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References

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