Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Antioxidant and Anti-dyslipidemic Effect of Artemisiae iwayomogii Herba, Curcumae longae Radix, and Plantaginis Semen Complex Extract(ACP) on HepG2 Cells

HepG2 cell에서 한인진, 울금, 차전자 추출물(ACP)의 항산화 및 항이상지질혈증 효과

  • Jung, Eunsun (Department of Circulatory Internal Medicine, College of Oriental Medicine, Daejeon University) ;
  • Cho, Hyun Kyoung (Department of Circulatory Internal Medicine, College of Oriental Medicine, Daejeon University) ;
  • Kim, Yoon Sik (Department of Circulatory Internal Medicine, College of Oriental Medicine, Daejeon University) ;
  • Yoo, Ho Ryong (Department of Circulatory Internal Medicine, College of Oriental Medicine, Daejeon University) ;
  • Seol, In Chan (Department of Circulatory Internal Medicine, College of Oriental Medicine, Daejeon University)
  • 정은선 (대전대학교 한의과대학 심계내과학 교실) ;
  • 조현경 (대전대학교 한의과대학 심계내과학 교실) ;
  • 김윤식 (대전대학교 한의과대학 심계내과학 교실) ;
  • 유호룡 (대전대학교 한의과대학 심계내과학 교실) ;
  • 설인찬 (대전대학교 한의과대학 심계내과학 교실)
  • Received : 2017.09.19
  • Accepted : 2017.12.07
  • Published : 2018.02.25
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Abstract

This study was performed to investigate the antioxidant and antidyslipidemic effects of Artemisiae iwayomogii Herba, Curcumae longae Radix and Plantaginis Semen complex extract(ACP) on HepG2 cells. We measured total polyphenols, total flavonoids, radical scavenging activity, and ABTS radical scavenging activity of ACP to evaluate its antioxidant activity. HepG2 cells were treated with ACP. Then, we evaluated ROS production; intracellular GSH content; GPx, GR, SOD, and catalase activities; free fatty acids and MDA levels; and mRNA expression levels of ACAT1 and HMG-CoA reductase. Results: ACP contains polyphenols and flavonoids and increased the DPPH and ABTS radical scavenging activities in HepG2 cells in a dose dependent manner. Also, ACP significantly reduced ROS production in HepG2 cells compared to the control group and significantly increased the GSH content, and elevated the enzyme activities of GPx, GR, and catalase in HepG2 cells compared to the control group. In addition, ACP reduced the mRNA expression of ACAT1 and HMG-CoA reductase in HepG2 cells compared to that in the control group. Conclusion: These results suggest that ACP has an antioxidant effect and may suppress the expression of dyslipidemia - associated genes and thus may be useful for the improvement of dyslipidemia.

Keywords

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