The Korean Journal of Physiology and Pharmacology

The Korean Society of Pharmacology (대한약리학회)
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Anti-inflammatory and utero-relaxant effect of α-bisabolol on the pregnant human uterus

  • Munoz-Perez, Victor Manuel (Area Academica de Medicina del Instituto de Ciencias de la Salud. Universidad Autonoma del Estado de Hidalgo) ;
  • Ortiz, Mario I. (Area Academica de Medicina del Instituto de Ciencias de la Salud. Universidad Autonoma del Estado de Hidalgo) ;
  • Ponce-Monter, Hector A. (Area Academica de Medicina del Instituto de Ciencias de la Salud. Universidad Autonoma del Estado de Hidalgo) ;
  • Monter-Perez, Vicente (Hospital General de los SSH) ;
  • Barragan-Ramirez, Guillermo (Hospital General de los SSH)
  • Received : 2017.11.02
  • Accepted : 2018.02.09
  • Published : 2018.07.01
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Abstract

The aim of this study was to evaluate the in vitro anti-inflammatory and utero-relaxant effect of ${\alpha}$-bisabolol on the pregnant human myometrium. Samples from the pregnant human myometrium were used in functional tests to evaluate the inhibitory effect of ${\alpha}$-bisabolol (560, 860, 1,200 and $1,860{\mu}M$) on spontaneous myometrial contractions. The intracellular cyclic adenosine monophosphate (cAMP) levels generated in response to ${\alpha}$-bisabolol in human myometrial homogenates were measured by ELISA. The anti-inflammatory effect of ${\alpha}$-bisabolol was determined through the measurement of two pro-inflammatory cytokines, tumor necrosis factor-${\alpha}$ ($TNF{\alpha}$) and interleukin $(IL)-1{\beta}$, and the anti-inflammatory cytokine IL-10, in pregnant human myometrial explants stimulated with lipopolysaccharide (LPS). Forskolin was used as a positive control to evaluate the cAMP and cytokine levels. ${\alpha}$-Bisabolol was found to induce a significant inhibition of spontaneous myometrial contractions at the highest concentration level (p<0.05). ${\alpha}$-Bisabolol caused a concentration-dependent decrease in myometrial cAMP levels (p<0.05) and a concentration-dependent decrease in LPS-induced $TNF{\alpha}$ and $IL-1{\beta}$ production, while IL-10 production did not increase significantly (p>0.05). The anti-inflammatory and utero-relaxant effects induced by ${\alpha}$-bisabolol were not associated with an increase in cAMP levels in pregnant human myometrial samples. These properties place ${\alpha}$-bisabolol as a potentially safe and effective adjuvant agent in cases of preterm birth, an area of pharmacological treatment that requires urgent improvement.

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