Asian-Australasian Journal of Animal Sciences

  • 제31권9호
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  • Pages.1507-1515
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  • 2018
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  • 1011-2367(pISSN)
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  • 1976-5517(eISSN)
아세아태평양축산학회 (Asian Australasian Association of Animal Production Societies)
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Muscle differentiation induced up-regulation of calcium-related gene expression in quail myoblasts

  • Park, Jeong-Woong (Institute of Green-Bio Science and Technology, Seoul National University) ;
  • Lee, Jeong Hyo (Institute of Green-Bio Science and Technology, Seoul National University) ;
  • Kim, Seo Woo (Graduate School of International Agricultural Technology, Seoul National University) ;
  • Han, Ji Seon (Graduate School of International Agricultural Technology, Seoul National University) ;
  • Kang, Kyung Soo (Bio Division, Medikinetics, Inc.) ;
  • Kim, Sung-Jo (Division of Cosmetics and Biotechnology, Hoseo University) ;
  • Park, Tae Sub (Institute of Green-Bio Science and Technology, Seoul National University)
  • 투고 : 2018.04.16
  • 심사 : 2018.05.29
  • 발행 : 2018.09.01
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초록

Objective: In the poultry industry, the most important economic traits are meat quality and carcass yield. Thus, many studies were conducted to investigate the regulatory pathways during muscle differentiation. To gain insight of muscle differentiation mechanism during growth period, we identified and validated calcium-related genes which were highly expressed during muscle differentiation through mRNA sequencing analysis. Methods: We conducted next-generation-sequencing (NGS) analysis of mRNA from undifferentiated QM7 cells and differentiated QM7 cells (day 1 to day 3 of differentiation periods). Subsequently, we obtained calcium related genes related to muscle differentiation process and examined the expression patterns by quantitative reverse-transcription polymerase chain reaction (qRT-PCR). Results: Through RNA sequencing analysis, we found that the transcription levels of six genes (troponin C1, slow skeletal and cardiac type [TNNC1], myosin light chain 1 [MYL1], MYL3, phospholamban [PLN], caveolin 3 [CAV3], and calsequestrin 2 [CASQ2]) particularly related to calcium regulation were gradually increased according to days of myotube differentiation. Subsequently, we validated the expression patterns of calcium-related genes in quail myoblasts. These results indicated that TNNC1, MYL1, MYL3, PLN, CAV3, CASQ2 responded to differentiation and growth performance in quail muscle. Conclusion: These results indicated that calcium regulation might play a critical role in muscle differentiation. Thus, these findings suggest that further studies would be warranted to investigate the role of calcium ion in muscle differentiation and could provide a useful biomarker for muscle differentiation and growth.

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참고문헌

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