Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Protective Effects of Chijabaegpi-tang on Atopic Dermatitis in TNF-α/IFNγ-induced HaCaT Cells

피부각질세포에서 치자백피탕(梔子柏皮湯)의 아토피 피부염 개선효과

  • Eun, So Young (Department of Phygiology, College of Korean Medicine, Wonkwnag University) ;
  • Yoon, Jung Joo (Department of Phygiology, College of Korean Medicine, Wonkwnag University) ;
  • Kim, Hye Yoom (Department of Phygiology, College of Korean Medicine, Wonkwnag University) ;
  • Ahn, You Mee (Department of Phygiology, College of Korean Medicine, Wonkwnag University) ;
  • Han, Byung Hyuk (Department of Phygiology, College of Korean Medicine, Wonkwnag University) ;
  • Hong, Mi Hyeon (Department of Phygiology, College of Korean Medicine, Wonkwnag University) ;
  • Son, Chan Ok (Department of Phygiology, College of Korean Medicine, Wonkwnag University) ;
  • Na, Se Won (Department of Phygiology, College of Korean Medicine, Wonkwnag University) ;
  • Lee, Yun Jung (Department of Phygiology, College of Korean Medicine, Wonkwnag University) ;
  • Kang, Dae Gill (Department of Phygiology, College of Korean Medicine, Wonkwnag University) ;
  • Lee, Ho Sub (Department of Phygiology, College of Korean Medicine, Wonkwnag University)
  • 은소영 (원광대학교 한의과대학 생리학교실) ;
  • 윤정주 (원광대학교 한의과대학 생리학교실) ;
  • 김혜윰 (원광대학교 한의과대학 생리학교실) ;
  • 안유미 (원광대학교 한의과대학 생리학교실) ;
  • 한병혁 (원광대학교 한의과대학 생리학교실) ;
  • 홍미현 (원광대학교 한의과대학 생리학교실) ;
  • 손찬옥 (원광대학교 한의과대학 생리학교실) ;
  • 나세원 (원광대학교 한의과대학 생리학교실) ;
  • 이윤정 (원광대학교 한의과대학 생리학교실) ;
  • 강대길 (원광대학교 한의과대학 생리학교실) ;
  • 이호섭 (원광대학교 한의과대학 생리학교실)
  • Received : 2018.03.15
  • Accepted : 2018.08.27
  • Published : 2018.08.25
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Abstract

Chijabaegpi-tang (CHG) is an oriental herbal medicine that has been used for its various pharmacological effects, which include anti-inflammatory, anti-oxidant and immunoregulation activities. In the present study, we investigated which skin inflammations are involved in the $TNF-{\alpha}/IFN{\gamma}$-induced HaCaT cells. We investigated the suppressive effect of CHG on $TNF-{\alpha}/IFN{\gamma}$-induced HaCaT cell production of the following chemokines: macrophage-derived chemokine (MDC)/CCL22; regulated on activation, normal T-cell expressed and secreted (RANTES)/CCL5; and interleukin-8 (IL-8); thymus and activation-regulated chemokine (TARC)/CCL17. The pre-treatment of HaCaT cells with CHG suppressed $TNF-{\alpha}/IFN{\gamma}$-induced nuclear transcription factor kappa-B ($NF-{\kappa}B$). In addition, CHG inhibited $TNF-{\alpha}/IFN{\gamma}$-induced phosphorylation of ERK and p38. $TNF-{\alpha}/IFN{\gamma}$ suppressed the expression of skin barrier proteins, including filaggrin (FLG), Involucrin (IVL) and loricrin (LOR). By contrast, CHG restored the expression of FLG, IVL and LOR. Taken together, our findings suggest that CHG could be a therapeutic agent for prevention of skin disease, including atopic dermatitis.

Keywords

References

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