Journal of the Korean Society of Food Culture (한국식생활문화학회지)

Korean Society of Food Culture (한국식생활문화학회)
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The Effects of α-Lipoic Acid in Adilution Solvents, Dose- and Time-dependent Manner on Cell Growth Blocking in 3T3-L1

α-Lipoic acid의 희석용매, 처리농도, 처리시간에 따른 3T3-L1 지방세포 성장에 미치는 영향

  • Seo, Eunyoung (Department of Food and Nutrition, Jangan University)
  • 서은영 (장안대학교 식품영양과)
  • Received : 2018.09.27
  • Accepted : 2018.10.18
  • Published : 2018.10.31
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Abstract

Purpose: This study examined the effects of ${\alpha}$-lipoic acid in diluted solvents on cell growth in 3T3-L1 cells according to the treated concentration and times. Methods: Adipocyte 3T3-L1 cell were cultured. Confluent cells underwent starvation with SFM for 1 day and then were cultured in a medium containing various concentrations 0, 100, 200, and $400{\mu}mol/L$ of ${\alpha}$-lipoic acid. The cell viability was measured using the EZ Cytox assay kit. In addition, the effect of ${\alpha}$-lipoic acid of diluted solvents on the cell growth in 3T3-L1cells was examined according to the treated concentration and times. Results: The ${\alpha}$-lipoic acid diluted ethanol inhibited cell proliferation in a dose and time dependent manner. The ${\alpha}$-lipoic acid diluted ethanol induced adipocyte 3T3-L1 cells proliferation with an adipocyte inducer. In addition, ${\alpha}$-lipoic acid inhibited adipocyte 3T3-L1 growth in a dose and time dependent manner (p<0.05). Conclusion: This study showed that a treatment with ${\alpha}$-lipoic acid diluted ethanol inhibits cell growth of, adipocyte 3T3-L1 cells induced with an adipocyte inducer, ($200{\mu}mol/L$ of ${\alpha}$-lipoic acid) treated for 48 hr.

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References

  1. Snell EE, Strong FM, Peterson WH. 1937. Growth factors for bacteria: Fractionation and properties of an accessory factor for lactic acid bacteria. Biochem J., 31(10):1789-1799 https://doi.org/10.1042/bj0311789
  2. Gleiter CH, Schug BS, Hermann R, Elze M, Blume HH, Gundert-Remy U. 1996. Influence of food intake on the bioavailability of thioctic acid enantiomers. Eur J Clin Pharmacol., 50(6):513-514 https://doi.org/10.1007/s002280050151
  3. Teichert J, Kern J, Tritschler HJ, Ulrich H, Preiss R. 1998. Investigations on the pharmacokinetics of alpha-lipoic acid in healthy volunteers. Int J Clin Pharmacol Ther., 36(12):625-628
  4. Breithaupt-Grogler K, Niebch G, Schneider E, Erb K, Hermann R, Blume HH, Schug BS, Belz GG. 1999. Doseproportionality of oral thioctic acid-coincidence of assessments via pooled plasma and individual data. Eur J Pharm Sci., 8(1):57-65 https://doi.org/10.1016/S0928-0987(98)00061-X
  5. Reed LJ. 1957. The chemistry and function of lipoic acid. Adv Enzymol Relat Subj Biochem., 18:319-347
  6. Rosenberg HR, Culik R. 1959. Effect of alpha-lipoic acid on vitamin C and vitamin E deficiencies. Arch Biochem Biophys., 80-86
  7. Jordan SW, Cronan JE Jr. 1997. Biosynthesis of lipoic acid and posttranslational modification with lipoic acid in Escherichia coli. Methods Enzymol., 279:176-183
  8. Wada H, Shintani D, Ohlrogge J. 1997. Why do mitochondria synthesize fatty acids? Evidence for involvement in lipoic acid production. Proc Natl Acad Sci USA., 18; 94(4):1591-1596 https://doi.org/10.1073/pnas.94.4.1591
  9. Maliska D, Winiarska K, 2005. Lipoic acid : characteristic and therapeutic application. Postepy Hig Med Dosw., 59:535-543
  10. Lee WJ, Lee IK, Kim HS, Kim YM, Koh EH, Won JC, Han SM, Kim MS, Jo I, Oh GT, Park IS, Youn JH, Park SW, Lee KU, Park JY. 2005. Alpha-lipoic acid prevents endothelial dysfunction in obese rats via activation of AMP-activated protein kinase. Arterioscler Thromb Vasc Biol., 25(12):2488-2494 https://doi.org/10.1161/01.ATV.0000190667.33224.4c
  11. Kim MS, Park JY, Namkoong C, Jang PG. 2004. Anti-obesity effects of alpha-lipoic acid mediated by suppression of hypothalamic AMP-activated protein kinase. Nature medicine., 10(7):727-733 https://doi.org/10.1038/nm1061
  12. Yi X, Pashai A, Xia M, Moreau R. 2013. Reveral of obesityinduced hypertriglyceridemia by (R)-$\alpha$-lipoic acid in ZDF (fa/fa) rats. Biochem Biophys Res Commun., 439(3):390-395 https://doi.org/10.1016/j.bbrc.2013.08.063
  13. Saengsirisuwan V, Perez FR, Sloniger JA, Maier T, Henriksen EJ. 2004. Interactions of exercise training and alpha-lipoic acid on insulin signaling in skeletal muscle of obese Zucker rats. Am J Physiol Endocrinol Metab., 287(3):E529-536 https://doi.org/10.1152/ajpendo.00013.2004
  14. Kim DM, Ahn CW, Nam SY. 2005. Prevalence of obesity in Korea. Obes Rev., 6(2):117-121 https://doi.org/10.1111/j.1467-789X.2005.00173.x
  15. Hirsch J, Batchelor B. 1976. Adipose tissue cellularity in human obesity. Clin Endocrinol Metab., 5(2):299-311 https://doi.org/10.1016/S0300-595X(76)80023-0
  16. Green H, Kehinde O. 1975. An established preadipose cell line and its differentiation in culture. II. Factors affecting the adipose conversion. Cell., 5(1):19-27 https://doi.org/10.1016/0092-8674(75)90087-2
  17. Lee MS, Kim CT, Kim CJ, Cho YJ, Kim YH. 2006. Efeects of Portulaca Oleracea L. Extract on lipolysis and hormone sensitive lipase (HSL) gene expression in 3T3-L1 adipocytes. Korean J Nutr., 39(8):742-747
  18. Seo EY. 2015. Effects of (6)-gingerol, ginger component on adipocyte development and differentiation in 3T3-L1. J Nutri Health., 48(4):327-334 https://doi.org/10.4163/jnh.2015.48.4.327
  19. Yeh WC, Cao Z, Classon SL, McKnight SL. 1995. Cascade regulation of terminal adipocyte differentiation by three member of the C/EBP family of leucin zipper proteins. Gene., 9:168-181 https://doi.org/10.1101/gad.9.2.168
  20. Chu SK, Seo EY, Kim WK, Kang NE. 2008. Effect of cyanidin on cell motility and invasion in MDA-MB-231 human breast cancer cells. Korean J Nutr., 41(8):711-717
  21. Davis GDJ, Masilamoni JG, Arul V, Sakthivelu IV, Kumar MSM, Baraneedharan U, Paul SFD. 2009. Radioprotective effect of DL-$\alpha$-lipoic acid on mice skin fibroblasts. Cell Biol Toxicol., 25:331-340 https://doi.org/10.1007/s10565-008-9087-5
  22. Koh JM, Lee YS, Byun CH, Chang EJ, Kim H, Kim YH, Kim HH, Kim GS. 2005. Alpha-lipoic acid suppresses osteoclastogenesis despite increasing the receptor activator of nuclear factor kappaB ligand/osteoprotegerin ratio in human bone marrow stromal cells. J Endocrinol., 185(3):401-413 https://doi.org/10.1677/joe.1.05995
  23. Artwohl M, Muth K, Kosulin K, de Martin R, Holzenbein T, Rainer G, Freudenthaler A, Huttary N, Schmetterer L, Waldhausl WK, Baumgartner-Parzer SM. 2007. R-(+)-alpha-lipoic acid inhibits endothelial cell apoptosis and proliferation: involvement of Akt and retinoblastoma protein/E2F-1. Am J Physiol Endocrinol Metab., 293(3):E681-689 https://doi.org/10.1152/ajpendo.00584.2006
  24. Han D, Sen CK, Roy S, Kobayashi MS, Tritschler HJ, Packer L. 1997. Protection against glutamate-induced cytotoxicity in C6 glial cells by thiol antioxidants. Am J Physiol., 273: R1771-R1778
  25. Ghibu S, Richar C, Delemasure S, Vergely C, Mogosan C, Muresan A. 2008. An endogenous dithiol with antioxidant properties: Alpha-lipoic acid, potential uses in cardiovascular disease. Elsevier Masson SAS., 57:161-165
  26. Brooks GA, Casey MD, Timothy PW. 1984. Estimation of anaerobic energy production and efficiency in rat during exercise. J Appl Physiol, 56:520-525 https://doi.org/10.1152/jappl.1984.56.2.520
  27. Pari L, Murugavel P. 2004. Protective effect of alpha lipoic acid against chloroquine-induced hepatotoxicity in rats. J Appl Toxicol., 24:21-26 https://doi.org/10.1002/jat.940
  28. Packer L, Witt EH, Tritschler HJ. 1995. Alpha-lipoic acid as a biological antioxidant. Free Radic Biol Med., 19:227-250 https://doi.org/10.1016/0891-5849(95)00017-R
  29. Hahm JR, Noh HS, Ha JH, Roh GS, Kim DR. 2014. Alpha-lipoic acid attenuates adipocyte differentiation and lipid accumulation in 3T3-L1 cells vi dependent autophagy. Life science., 100(2):124-132
  30. Prieto-Hontoria PL, Fernandez-Galilea M, Perez-Matute P, Martinez JA, Moreno-Aliaga M. 2013. Lipoic acid inhibits adiponectin production in 3T3-L1 adipocyte. J Physiol and Biochem., 69(3):595-600 https://doi.org/10.1007/s13105-012-0230-7