동의생리병리학회지 (Journal of Physiology & Pathology in Korean Medicine)

  • 제32권5호
  • /
  • Pages.321-327
  • /
  • 2018
  • /
  • 1738-7698(pISSN)
  • /
  • 2288-2529(eISSN)
대한동의생리학회·한의병리학회 ( The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine)
권호 표지
DOI QR코드

DOI QR Code

토사자 에탄올 추출물이 UVB로 유도된 CCD-986Sk cell에서 주름개선 생리지표에 미치는 영향

fects of Cuscuta Chinensis Lamark Ethanol Extract on Wrinkle Improvement Bio-markers by UVB-induced CCD-986Sk Cell

  • 주인환 (대전대학교 한의과대학 병리학교실) ;
  • 최학주 (대전대학교 난치성 면역질환의 동서생명의학연구 지역혁신센터) ;
  • 심부용 (대전대학교 난치성 면역질환의 동서생명의학연구 지역혁신센터) ;
  • 민가율 (대전대학교 한의과대학 병리학교실) ;
  • 김동희 (대전대학교 한의과대학 병리학교실)
  • Joo, In Hwan (Department of Pathology, College of Korean Medicine, Daejeon University) ;
  • Choi, Hak Joo (Traditional and Biomedical Research Center(TBRC-RIC), Daejeon University) ;
  • Sim, Boo Yong (Traditional and Biomedical Research Center(TBRC-RIC), Daejeon University) ;
  • Min, Ga Yul (Department of Pathology, College of Korean Medicine, Daejeon University) ;
  • Kim, Dong Hee (Department of Pathology, College of Korean Medicine, Daejeon University)
  • 투고 : 2018.07.10
  • 심사 : 2018.09.11
  • 발행 : 2018.10.25
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

The purpose of this study was to investigate the effects of Cuscuta chinensis Lamark ethanol extract (CL) on wrinkle improvement. Cuscuta chinensis Lamark is known to contain dried saccharide, alkaloids, flavonoids, lignans and rein glycoside as major components of dried mature seeds of Cuscuta japonica Choisy. In this study, we evaluated the anti-wrinkle effects of CL and investigated bio-markers (e.g ; MMP-1, MMP-3, MMP-9, TIMP-1, type I procollagen) associated with skin wrinkle improvement. We tested the anti-wrinkle effect of CL using human fibroblast called CCD-986Sk cell. We observed an increase in MMPs, TIMP-1, and type 1 pro-collagen CL in CCD-986Sk cells irradiated with UVB at an intensity of $2mJ/cm^2$ for 60 seconds. As a result, CL decreased UVB-induced MMPs levels and mRNA expressions in CCD-986Sk cell. The levels and mRNA expressions of type I procollagen and TIMP-1 were increased by CL. These results suggest that CL has activities on improvement of skin wrinkle, which is induced by UVB radiation. Taken together, this study proposed the possibility of developing herbal medicine and functional herbal cosmetic materials with wrinkle-improving effects of Cuscuta chinensis Lamark.

키워드

참고문헌

  1. Lee KM, Lim SC, Kim JS, Lee BH. A clinical study on facial wrinkles treated with miso facial acupuncture-measured by the facial skin photographing system. Journal of Korean Acupuncture & Moxibustion Society. 2010;27(1):101-7.
  2. Kim YJ, Yoon YM. Regulation of Col1A1 and MMP1 expression by taurine, major component of oyster, in human dermal fibroblasts. Korean Journal of Aesthetics and Cosmetics Society. 2013;11(2):393-7.
  3. Yoon YM, Choi SJ, Park WJ, Eom SY, Lee SK, et al. The Protective Effect of Extracts of Bifidobacterium longum against ultraviolet B in human dermal fibroblasts. Korean Journal of Aesthetics and Cosmetics Society. 2012;10(4):887-91.
  4. Seite S, Zucchi H, Septier D, Igondjo-Tchen S, Senni K, et al. Elastin changes during chronological and photo ageing: the important role of lysozyme. Journal of the European Academy of Dermatology and Venereology. 2006;20(8):980-7. https://doi.org/10.1111/j.1468-3083.2006.01706.x
  5. Wlaschek M, Tantcheva-Poor I, Naderi L, Ma W, Schneider LA, et al. Solar UV irradiation and dermal photoaging. Journal of Photochemistry and Photobiology B: Biology. 2001;63(1-3):41-51. https://doi.org/10.1016/S1011-1344(01)00201-9
  6. Kim YA, Kim DH, Kim YA, Yu JM, Park BJ, et al. Anti-aging effects of casuarictin on UVB-induced CCD-986sk cell. Journal of Applied Biological Chemistry. 2017;60(1):13-7. https://doi.org/10.3839/jabc.2017.003
  7. Vile GF, Tyrrell RM. UVA radiation-induced oxidative damage to lipids and proteins in vitro and in human skin fibroblasts is dependent on iron and singlet oxygen. Free Radical Biology and Medicine. 1995;18(4):721-30. https://doi.org/10.1016/0891-5849(94)00192-M
  8. So SH, Lee SK, Hwang EI, Koo BS, Han GH, et al. Mechanisms of Korean red ginseng and herb extracts (KTNG0345) for anti-wrinkle activity. Journal of Ginseng Research. 2008;32(1):39-47. https://doi.org/10.5142/JGR.2008.32.1.039
  9. Fisher GJ, Datta SC, Talwar HS, Wang ZQ, Varani J, et al. Molecular basis of sun-induced premature skin ageing and retinoid antagonism. Nature. 1996;379(6563):335. https://doi.org/10.1038/379335a0
  10. Kwon SB. Effects of Phytosphingosine-1-phosphate and Epidermal Growth Factor on Skin Wrinkles and Elasticity Improvement. Graduate School of Konkuk University. 2014.
  11. Jin SS. Study on the Effect of Semen Cuscutae. Graduate School of Wonkwang University. 2001.
  12. Heo, J. DongEuiBoGam. DongEuiBoGamBooks. 2006;2025-6.
  13. Kim HH. A study of medicinal herbs available for a skin beauty treatment and the efficacy. Graduate School of Catholic University. 2003.
  14. Song JS. Oriental skin beauty. Hyorim, 1993.
  15. Hongzhu, G., Jiashi, L. Study on constituents of the seed from Cuscuta australis. Beijing University of Traditional Chinese Medicine. 2000;23(3):20-2.
  16. MIYAHARA K, Du XM, WATAMABE M, Sugimura C, Yahara S, Nohara T. Resin glycosides. XXIII. Two novel acylated trisaccharides related to resin glycoside from the seeds of Cuscuta chinensis. Chemical and pharmaceutical bulletin. 1996;44(3):481-5. https://doi.org/10.1248/cpb.44.481
  17. Lee MY, Jeon SJ. Effect of Cuscutae extract on experimentally induced liver damage in rabbits. Journal of the Korean Society of Food Science and Nutrition. 1989;18:333-7.
  18. Han JW. Effects of semen cuscutae aqua-acupuncture on the blood pressure in spontaneously hypertensive rats. Graduate School of Wooseok University. 1998.
  19. Kim EJ. Anti-hyperlipidemic effects of Cuscutae Semen. Graduate School of Sookmyung Women's University. 1996.
  20. Jeon YH, Kim MH, Kim MR. Antioxidative and Antimutagenic Activity of Ethanol Extracts from Cuscutae Semen Korean Journal of Food & Cookery Science. 2008;24:46-51.
  21. Lee SH. Stadies on the Growth-Inhibitory Effects of Cuscuta japonica Choisy's Extracts against Propionibacterium acnes. Graduate School of Catholic University. 2002.
  22. Joe WA, Cheon SJ, Jang MJ, Sung JY, Jung SH, et al. Study of the Cosmeceutical Activities of Cuscuta japonica Choisy. The Korea journal of herbology. 2006;21:103-8.
  23. Chang SJ, Suk KD. Inhibitory Effects on Melanin Biosynthesis and Tyrosinase Activity, Cytotoxicity in Clone M-3 and Antioxidant Activity by Cuscuta japonica, C. australis, and C. chinensis Extracts. Yakhak Hoeji. 2006;50:421-8.
  24. Kim SE, Li SH. The Effect of Semen cuscutae Extracts on the Acne Skin. Korean Journal of Aesthetics and Cosmetics Society. 2012;10(3):493-501.
  25. Ichihashi M, Ueda M, Budiyanto A, Bito T, Oka M, et al. UV-induced skin damage. Toxicology. 2003;189(1-2):21-39. https://doi.org/10.1016/S0300-483X(03)00150-1
  26. Lee H, Bae SK, Pyo M, Heo Y, Kim CG, Kang C, Kim E. Anti-wrinkle effect of PLA2-free bee venom against UVB-irradiated human skin cells. Journal of Agriculture and Life Science. 2015;49(1):125-35. https://doi.org/10.14397/jals.2015.49.1.125
  27. Joo DH, Yoo DH, Lee JY. A Study on the Anti-wrinkle Activities of Sesamum indicum L. Ethanol Extracts on CCD-986sk. Journal of the society of cosmetic scientists of korea. 2016;42(4):377-85. https://doi.org/10.15230/SCSK.2016.42.4.377
  28. Park MA, Sim MJ, Kim YC. Anti-photoaging effects of Angelica acutiloba root ethanol extract in human dermal fibroblasts. Toxicological research. 2017;33(2):125. https://doi.org/10.5487/TR.2017.33.2.125
  29. Kang KS, Kim ID, Kwon RH, Heo YY, Oh SH, et al. The evaluation of anti-wrinkle effects in oriental herb extract. Journal of Life Science. 2007;17(8):1147-51. https://doi.org/10.5352/JLS.2007.17.8.1147
  30. Burns T, Breathnach S, Cox N, Griffiths C. Textbook of dermatology. Blackwell. 2004;49:32-49.
  31. Scharffetter-Kochanek K, Brenneisen P, Wenk J, Herrmann G, Ma W, Kuhr L, Meewes C, Wlaschek M. Photoaging of the skin from phenotype to mechanisms. Experimental gerontology. 2000;35(3):307-16. https://doi.org/10.1016/S0531-5565(00)00098-X
  32. Ahn SY. An opening step of cosmetic dermatology. Shinheung MedScience. 2003.
  33. Yamamoto T, Eckes B, Mauch C, Hartmann K, Krieg T. Monocyte chemoattractant protein-1 enhances gene expression and synthesis of matrix metalloproteinase-1 in human fibroblasts by an autocrine $IL-1{\alpha}$ loop. The Journal of Immunology. 2000;164(12):6174-9. https://doi.org/10.4049/jimmunol.164.12.6174

피인용 문헌

  1. Quality Characteristics of Yakju (Korean Traditional Rice Wine) Supplemented with Varying Amounts of Cuscuta Seed (Cuscuta chinensis Lamark) vol.30, pp.6, 2018, https://doi.org/10.17495/easdl.2020.10.30.6.409