동의생리병리학회지 (Journal of Physiology & Pathology in Korean Medicine)

  • 제32권5호
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  • Pages.333-340
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  • 2018
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  • 1738-7698(pISSN)
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  • 2288-2529(eISSN)
대한동의생리학회·한의병리학회 ( The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine)
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가미귀비탕 탕액과 시판제제의 성분 및 생리활성 비교

Comparison of Marker Components and Biological Activities of Gamiguibi-tang(Jiaweiguipi-tang) Decoction and Commercial Extract Granules

  • Kim, Jung Ok (Traditional Korean Medicine Technology Division, National Development Institute of Korean Medicine) ;
  • Baek, Ka Yeon (Traditional Korean Medicine Technology Division, National Development Institute of Korean Medicine) ;
  • Lee, Hwa Dong (Traditional Korean Medicine Technology Division, National Development Institute of Korean Medicine)
  • 투고 : 2018.07.04
  • 심사 : 2018.10.24
  • 발행 : 2018.10.25
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초록

Gamiguibi-tang (GGBT) is a traditional herbal medicine generally used to treat anemia, insomnia, anxiety, and nervousness. GGBT is being commercially produced in the form of extract granule and the quality control methods are specified in the Korean Herbal Pharmacopeia (KHP). However, there is no method to simultaneously analyze compound preparations. In this study, a HPLC method was developed and validated for the simultaneous determination of marker compounds in GGBT. And the contents of marker components and biological activities of the commercial GGBT extract granules (GGBT-2 and GGBT-3) were compared with those of the GGBT decoction (GGBT-1). We confirmed the robustness of simultaneous analytical method by monitoring the contents of the commercial GGBT products and carrying out validation. The marker components of GGBT were geniposide ($8.03{\sim}12.70{\mu}g/mL$), paeoniflorin ($2.79{\sim}4.25{\mu}g/mL$) and glycyrrhizic acid ($5.06{\sim}6.30{\mu}g/mL$). DPPH and ABTS radical scavenging activities were 47.34~63.17% and 21.52~33.61% in the GGBT products concentration of $1,000{\mu}g/mL$, respectively. The GGBT products significantly decreased NO, iNOS and COX-2 production in lipopolysaccharide (LPS)-induced RAW 264.7 macrophages in a concentration-dependent manner. The GGBT-2 had higher contents of marker components and biological activities than GGBT-1 and GGBT-3. The research suggest that be used in developing quality control methods for enhancing the quality of herbal medicines.

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참고문헌

  1. Park HM, Choi KH, Jung JY, Lee SD. Metal exposure through consumption of herbal medicine, and estimation of health risk among Korean population. Kor J Env Health. 2006;32(2):186-91.
  2. Lim JW, Kim JW, Chung SY, Cho SH, Oh MS, Hwang WW. The Antioxidative and Neuroprotective Effect of Guibi-tang (Guipitang) and Guibi-tang gamibang (Guipitang jiaweijang) on PC12 cells. J Oriental Neuropsychiatry. 2009;20(1):1-19.
  3. Yoo DY, Lim DW, Jeong JH. Bibliographical study on Kwibitang applied in gynecological diseases. Korean J Orien Gynecol. 1998;11(2):165-77.
  4. Huh J. Donguibogam, Seoul : Namsandang publisher. 1980. p. 98.
  5. Park SD, Park HJ, Ju WS. Study on antioxidant activities of Guibitang and its component groups. Kor J Herbol. 2001;16(1):11-28.
  6. Lim DW, Kim DH, You DY. Effects of Kamiquibitang (KGT) on immunologic control function. Korean J Orien Gynecol. 1999;12(2):253-80.
  7. Kim GY, Park JI, Ko H, Kim KT, Shin SM. A Case Report of an Insomnia Patient Treated with Gamiguibi-tang. J Int Korean Med. 2016;37(5):822-30. https://doi.org/10.22246/jikm.2016.37.5.822
  8. Kim MR, Kim YS, Lee YH, Lim EM. A Study about Effects of Gamiguibi-tang on Stress and Digestive Disorder of Women Students with Oligomenorrhea. J Korean Obstet Gynecol. 2013;26(2):104-19. https://doi.org/10.15204/jkobgy.2013.26.2.104
  9. Lee JA, Ban HR, Yang SJ, Park KM, Cho SH. Clinical study on one case of climacteric syndromes induced hysterectomy. Korean J Oriental Med Prescrip. 2004;12(1):263-76
  10. No DJ, Jung IC. Study on Effect to Serotonin Metabolism of Gamiguibi-tang on P815 Cell. J Oriental Neuropsychiatry. 2010;21(1):1-11.
  11. Kim EJ, Park HJ, Kim HJ, Kim JH, Ann JY, Lee JH, Kim YK. A monitoring study of marker contents in the Hwangnyeonhaedok-tang ex preparations on the market. Korean J Oriental Med Prescrip. 2008;16(1):95-107.
  12. Kim JH, Seo CS, Shin HK. The comparative study on decoctions of Ssanghwa-tang (Shuanghe-tang) extracted by different extraction conditions. Korean J Oriental Med Prescrip. 2010;18(2):125-34.
  13. Bi Z, Guo J, Zhang S, Li P. Study on the variation of the main constituents in Gardeniae Fructus after compatibility with Scutellariae Radix. Pharm Clini Res. 2011;19:29-31.
  14. Kim NM, Kim DH. Quality change of cinnamon extract prepared with various drying methods. Korean J Food & Nutr. 2000;13(2):152-7.
  15. Kim NM, Sung HS, Kim WJ. Effects of solvents and some extraction conditions on antioxidant activity in cinnamon extracts. Korean J Food Sci Technol. 1993;25:204-9.
  16. Korea Food and Drug Administration. The Korean Herbal Pharmacopoeia 11th ed. 2016.
  17. Hye Jin Yang, Jin Bae Weon, Jin Yeul Ma and Choong Je Ma. Simultaneous Determination of Six Bioactive Components in Guibi-tang by HPLC-DAD. Kor J Pharmacogn. 2010;41(4):313-8.
  18. Korea Food and Drug Administration. Analytical method guideline about validation of drug and etc. 2015.
  19. Blois MS. Antioxidant determinations by the use of a stable free radical. Nature. 1958;181:1199-200. https://doi.org/10.1038/1811199a0
  20. Re R, Pellegrini N, Proteggente A, Yang M, and Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biol Med. 1999;26(9-10):1231-7. https://doi.org/10.1016/S0891-5849(98)00315-3
  21. Jew SS, Bae ON and Chung JH. Anti-inflammatory effects of asiaticoside on inducible nitric oxide synthase and cyclooxygenase-2 in Raw-264.7 cell line. J Toxicol Pub Health. 2003;19(1):33-7.
  22. Yamamoto Y. He YP, Klein TW, Friedman H. Endotoxin induced cytotoxicity of macrophages is due to apoptosis caused by nitric oxide production. Journal of Endotoxin Research. 1994;1(3):181-7. https://doi.org/10.1177/096805199400100307
  23. Kim HK, Kim YE, Do JR, Lee YC, and Lee BY. Antioxidative activity and physiological activity of some Korean medicinal plants. Korean J Food Sci Technol. 1995;27(1):80-5.
  24. Aoshima H, Tsunoue H, Koda H, and Kiso Y. Aging of whiskey increases 1,1 diphenyl-2picrylhydrazyl radical scavenging activity. J Agric Food Chem. 2004;52(16):52404.
  25. Jeong JW, Lee YC, Jung SW, and Lee KM. Flavor components of citron juice as affected by the extraction method. Korean J Food Sci Technol. 1994;26(6):709-12.
  26. Yang HJ, Park MJ, Lee HS. Antioxidative activities and components of Gardenia jasminoides. Korean J Food Sci Technol. 2011;43(1):51-7. https://doi.org/10.9721/KJFST.2011.43.1.051
  27. Bang MH, Song JC, Lee SY, Park NK, Baek MI. Isolation and structure determination of antioxidants from the root of Paeonia lactiflora. J Korean Soc Agric Chem Biotechnol. 1999;42(2):170-5.
  28. Choi HJ, Lee WJ, Park SH, Song BW, Kim DH, Kim NJ. Studies on the processing of crude drug (IV) - Preparing standardization and regulation of stir-frying Glycyrrhizia root (1). Kor J Pharmacogn. 2005;36(3):209-19.
  29. Seo HS, Chung BH, Cho YG. Antioxidant and anticancer effects of Agrimony (Agrimonia pilosa L.) and Chinese lizardtail (Saururus chinesis Baill). Korean J Medicinal Crop Sci. 2008;16:139-43.
  30. Mariathasan S, Monack DM. Inflammasome adaptors and sensors; intracellular regulators of infection and inflammation. Nat Rev Immunol. 2007;7:31-40. https://doi.org/10.1038/nri1997
  31. Zedler S, Faist E. The impact of endogenous triggers on trauma-associated inflammation. Curr Opin Crit Care. 2006;12:595-601. https://doi.org/10.1097/MCC.0b013e3280106806
  32. Cho BO, Ryu HW, So Y, Lee CW, Jin CH, Yook HS, Jeong YW, Park JC, Jeong IY. Anti-Inflammatory Effect of Mangostenone F in Lipopolysaccharide-Stimulated RAW264.7 Macrophages by Suppressing $NF-{\kappa}B$ and MAPK Activation. Biomol Ther. 2014;22(4):288-94. https://doi.org/10.4062/biomolther.2014.052
  33. Iontcheva I, Amar S, Zawawi KH, Kantarci A and VanDyke TE. Role for moesin in lipopolysaccharide stimulated signal transduction. Infect Immun. 2004;72(4):2312-20. https://doi.org/10.1128/IAI.72.4.2312-2320.2004
  34. Hilliquin P, Borderie D, Hernvann A, Menkes CJ and Ekindjian OG. Nitric oxide as S-nitrosoproteins in rheumatoid arthritis. Arthritis Rheum. 1997;40(8):1512-7. https://doi.org/10.1002/art.1780400820
  35. Yoshitake J, Kato K, Yoshioka D, Sueishi Y, Sawa T, Akaike T, Yoshimura T. Suppression of NO production and 8-nitroguanosine formation by phenol containing endocrine disrupting chemicals in LPS-stimulated macrophages: involvement of estrogen receptor-dependent or independent pathways. Nitric Oxide. 2008;18(3):223-8. https://doi.org/10.1016/j.niox.2008.01.003
  36. Noh KH, Jang JH, Min KH, Chinzoring R, Lee MO, Song YS. Suppressive effect of green tea seed coat ethly acetate fraction on inflammation and its mechanism in RAW 264.7 macrophage cell. J Korean Soc Food Sci Nutr. 2011;40(5):625-34. https://doi.org/10.3746/jkfn.2011.40.5.625
  37. Rocca B, FitzGerald GA. Cyclooxygenases and prostaglandins: shaping up the immune response. Int Immunopharmacol. 2002;2(5):603-30. https://doi.org/10.1016/S1567-5769(01)00204-1