Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Effects of dietary glutamine and arginine supplementation on performance, intestinal morphology and ascites mortality in broiler chickens reared under cold environment

  • Received : 2017.02.28
  • Accepted : 2017.05.24
  • Published : 2019.01.01
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Abstract

Objective: An experiment was conducted to evaluate the effects of dietary glutamine (Gln) and arginine (Arg) supplementation on performance, intestinal morphology and ascites mortality in broilers. Methods: A total of 675 day old chicks were randomly allocated to 9 experimental groups in a $3{\times}3$ factorial arrangement based on a completely randomized design with 5 replicates of 15 chicks. Three levels of dietary Gln (0%, 0.5%, and 1%) and Arg (100%, 130%, and 160% of Ross recommendation) supplementation were used in ascites inducing condition ($15^{\circ}C{\pm}1^{\circ}C$) from 7 to 42 days of age. Results: Dietary supplementation of Gln increased body weight gain during grower, finisher and total periods (p<0.05) and increased feed intake during total period. Ascites mortality was decreased by Gln supplementation (p<0.05). Gln supplementation increased the villus height (VH) and crypt depth (CD) in duodenum and jejunum (p<0.05). Arg supplementation decreased CD in duodenum and jejunum, and increased ileum villus width (VW) and also VH/CD ratio in duodenum and jejunum (p<0.05). Both Gln and Arg increased the goblet cell number (GCN) in duodenum whereas Gln supplementation decreased GCN in jejunum and ileum (p<0.05). The $Gln{\times}Arg$ interaction were observed for right ventricle (RV)/total ventricular (TV) ratio, VH, VW, CD, VH/CD. Conclusion: It was concluded that dietary 0.5% Gln alone or along with 130% Arg of Ross requirement, improve the intestinal morphology and performance and hence decrease the ascites mortality in broiler chickens with cold induced ascites.

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References

  1. Balog JM. Ascites syndrome (pulmonary hypertension syndrome) in broiler chickens: are we seeing the light at the end of the tunnel? Avian Poult Biol Rev 2003;14:99-126. https://doi.org/10.3184/147020603783637490
  2. Luger D, Shinder D, Rzepakovsky V, Rusal M, Yahav S. Association between weight gain, blood parameters, and thyroid hormones and the development of ascites syndrome in broiler chickens. Poult Sci 2001;80:965-71. https://doi.org/10.1093/ps/80.7.965
  3. Yen JT, Nienaber JA, Hill DA, Pond WG. Oxygen consumption by portal vein-drained organs and by whole animal in conscious growing swine. Proc Soc Exp Biol Med 1989;190:393-8. https://doi.org/10.3181/00379727-190-42878
  4. Xiao L, Cao W, Liu G, et al. Arginine, N-carbamylglutamate, and glutamine exert protective effects against oxidative stress in rat intestine, Anim Nutr 2016;2:242-8. https://doi.org/10.1016/j.aninu.2016.04.005
  5. Santos FS, Farnell MB, Te'llez G, et al. Effect of prebiotic on gut development and ascites incidence of broilers reared in a hypoxic environment. Poult Sci 2005;84:1092-100. https://doi.org/10.1093/ps/84.7.1092
  6. Laudadio V, Passantino L, Perillo A, et al. Productive performance and histological features of intestinal mucosa of broiler chickens fed different dietary protein levels. Poult Sci 2012;91: 265-70. https://doi.org/10.3382/ps.2011-01675
  7. Jazideh F, Farhoomand P, Daneshyar M, Najafi G. The effects of dietary glutamine supplementation on growth performance and intestinal morphology of broiler chickens reared under hot conditions. Turkey J Vet Anim Sci 2014;38:264-70. https://doi.org/10.3906/vet-1210-32
  8. Khajali F, Heydary Moghaddam M, Hassanpour H. An L- Arginine supplement improves broiler hypertensive response and gut function in broiler chickens reared at high altitude. Int J Biometeorol 2014;58: 1175-9. https://doi.org/10.1007/s00484-013-0710-7
  9. Soltan MA. Influence of dietary glutamine supplementation on growth performance, small intestinal morphology, immune response and some blood parameters of broiler chickens. Int J Poult Sci 2009;8: 60-8. https://doi.org/10.3923/ijps.2009.60.68
  10. Murakami AE, Sakamoto MI, Natali MRM, Souza LMG, Franco JRG. Supplementation of glutamine and vitamin e on the morphometry of the intestinal mucosa in broiler chickens. Poult Sci 2007;86:488-95. https://doi.org/10.1093/ps/86.3.488
  11. Bartell SM, Batal AB. The effect of supplemental glutamine on growth performance, development of the gastrointestinal tract, and humoral immune response of broilers. Poult Sci 2007;86: 1940-7. https://doi.org/10.1093/ps/86.9.1940
  12. Newsholme P. Why is l-glutamine metabolism important to cells of the immune system in health, post injury, surgery or infection? J Nutr 2001;131:2515-22. https://doi.org/10.1093/jn/131.9.2515S
  13. Wu G, Bazer FW, Johnson GA, et al. Important roles for l-glutamine in swine nutrition and production. J Anim Sci 2011; 89:2017-30. https://doi.org/10.2527/jas.2010-3614
  14. Domeneghini C, Di Giancamillo A, Savoini G, et al. Structural patterns of swine ileal mucosa following l-glutamine and nucleotides administration during the weaning period. An histochemical and histometrical study. Histol Histopathol 2004; 19:49-58.
  15. Murakami AE, Fernandes JIM, Hernandes L, Santos TC. Effects of starter diet supplementation with arginine on broiler production performance and on small intestine morphometry. Pesq Vet Bras 2012;32:259-66. https://doi.org/10.1590/S0100-736X2012000300014
  16. Newsholme P, Brennnan L, Rubi B, Maechler P. New insights into amino acid metabolism, beta-cell function and diabetes. Clin Sci 2005;108:185-94. https://doi.org/10.1042/CS20040290
  17. Loser C, Eisel A, Harms D, Foelsch UR. Dietary polyamines are essential luminal growth factors for small intestinal and colonic mucosal growth and development. Gut 1999;44:12-6. https://doi.org/10.1136/gut.44.1.12
  18. Druyan S, Shlosberg A, Cahaner A. Evaluation of growth rate, body weight, heart rate, and blood parameters as potential indicators for selection against susceptibility to the ascites syndrome in young broilers. Poult Sci 2007;86:621-9. https://doi.org/10.1093/ps/86.4.621
  19. Daneshyar M, Kermanshahi H, Golian A. Changes of biochemical parameters and enzyme activities in broiler chickens with cold-induced ascites. Poult Sci 2009;88:106-10. https://doi.org/10.3382/ps.2008-00170
  20. Mohammadagheri N, Najafi R, Najafi GR. Effect of dietary supplementation of organic acids and phytase on performance and intestinal histomorphology of broilers. Vet Res Forum 2016;7:189-95.
  21. Nassiri Moghaddam H, Alizadeh-Ghamsari AH. Improved performance and small intestinal development of broiler chickens by dietary L-glutamine supplementation. J Appl Anim Res 2013;41:1-7. https://doi.org/10.1080/09712119.2012.738214
  22. Noy Y, Sklan D. Metabolic responses to early nutrition. J Appl Poult Res 1998;7:437-51. https://doi.org/10.1093/japr/7.4.437
  23. Kelly D, Smith JA, McCracken KJ. Digestive development of the early-weaned pig. 1. Effect of continuous nutrient supply on the development of the digestive tract and on changes in digestive enzyme activity uring the first week post-weaning. Br J Nutr 2001;65:169-80. https://doi.org/10.1079/BJN19910078
  24. Nkukwana TT, Muchenje V, Masika PJ, Mushonga B. Intestinal morphology, digestive organ size and digesta pH of broiler chickens fed diets supplemented with or without Moringa oleifera leaf meal. S Afr J Anim Sci 2015;45:362-70. https://doi.org/10.4314/sajas.v45i4.2
  25. Nascimento GM, Leandro NSM, Cafe MB, et al. Performance and intestinal characteristics of broiler chicken fed diet with glutamine in the diet without anticoccidials agents. Rev Bras Saude Prod Anim 2014;15:637-48. https://doi.org/10.1590/S1519-99402014000300011
  26. Fathi M, Tanha T, Daneshyar M. Effects of glutamine supplementation on growth performance, antioxidant status in broilers with pulmonary hypertension syndrome. Iran J Appl Anim Sci 2014;4:579-85.
  27. Mcbride BW, Kelly JM. Energy cost of absorption and metabolism in the ruminant gastrointestinal tract and liver: a review. J Anim Sci 1990; 68:2997-3010. https://doi.org/10.2527/1990.6892997x
  28. Saki AA, Haghighat M, Khajali F. Supplemental arginine administered in ovo or in the feed reduces the susceptibility of broilers to pulmonary hypertension syndrome. Br Poult Sci 2013;54:575-80. https://doi.org/10.1080/00071668.2013.811716
  29. Montagne L, Piel C, Lalles JP. Effect of diet on mucin kinetics and composition: Nutrition and health implications. Nutr Rev 62:105-14. https://doi.org/10.1111/j.1753-4887.2004.tb00031.x
  30. Fasina YO, Bowers JB, Hess JB, Mckee SR. Effect of dietary glutamine supplementation on Salmonella colonization in the ceca of young broiler chicks. Poult Sci 2010;89:1042-8. https://doi.org/10.3382/ps.2009-00415
  31. Tapiero H, Mathe G, Couvreur P, Tew KD. Free amino acids in human health and pathologies -II. Glutamine and glutamate. Biomed Pharmacother 2002;56:446-57. https://doi.org/10.1016/S0753-3322(02)00285-8
  32. McDonald P, Edwards PA, Greenhalgh JFD, Morgan CA. Metabolism. In: Animal nutrition. 6th ed. Pearson Education Limited; 2002. pp. 220-1.

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