Asian-Australasian Journal of Animal Sciences

  • 제32권2호
  • /
  • Pages.241-248
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  • 2019
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  • 1011-2367(pISSN)
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  • 1976-5517(eISSN)
아세아태평양축산학회 (Asian Australasian Association of Animal Production Societies)
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Isolation and characterization of a new Methanoculleus bourgensis strain KOR-2 from the rumen of Holstein steers

  • Battumur, Urantulkhuur (Graduate School of Future Convergence, Hankyong National University) ;
  • Lee, Manhee (Department of Animal Life and Environment Science, General Graduate School, Hankyong National University) ;
  • Bae, Gui Sek (Department of Animal Science and Technology, Chung-Ang University) ;
  • Kim, Chang-Hyun (Department of Animal Life and Environment Science, Hankyong National University)
  • 투고 : 2018.05.30
  • 심사 : 2018.07.06
  • 발행 : 2019.02.01
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초록

Objective: To isolate and identify new methanogens from the rumen of Holstein steers in Korea. Methods: Representative rumen contents were obtained from three ruminally cannulated Holstein steers ($793{\pm}8kg$). Pre-reduced media were used for the growth and isolation of methanogens. Optimum growth temperature, pH, and sodium chloride (NaCl) concentration as well as substrate utilization and antibiotic tolerance were investigated to determine the physiological characteristics of the isolated strain. Furthermore, the isolate was microscopically studied for its morphology. Polymerase chain reaction of 16S rRNA and mcrA gene-based amplicons was used for identification. Results: One strain designated as KOR-2 was isolated and found to be a non-motile irregular coccus with a diameter of 0.2 to $0.5{\mu}m$. KOR-2 utilized $H_2+CO_2$ and formate but was unable to metabolize acetate, methanol, trimethylamine, 2-propanol, and isobutanol for growth and methane production. The optimum temperature and pH for the growth of KOR-2 were $38^{\circ}C$ and 6.8 to 7.0, respectively, while the optimum NaCl concentration essential for KOR-2 growth was 1.0% (w/v). KOR-2 tolerated ampicillin, penicillin G, kanamycin, spectromycin, and tetracycline. In contrast, the cell growth was inhibited by chloramphenicol. Phylogenetic analysis of 16S rRNA and mcrA genes revealed the relatedness between KOR-2 and Methanoculleus bourgensis. Conclusion: Based on the physiological and phylogenetic characteristics, KOR-2 was thought to be a new strain within the genus Methanoculleus and named Methanoculleus bourgensis KOR-2.

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참고문헌

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