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Effects of Eucomiae Cortex on the Depression of Male Aged Mice

두충(杜沖)이 수컷 노화쥐의 우울증에 미치는 효과

  • Kim, Ho Hyun (Department of Physiology, College of Korean Medicine, Semyung University) ;
  • Ahn, Sang Hyun (Department of Anatomy, College of Korean Medicine, Semyung University) ;
  • Park, Sun Young (Department of Physiology, College of Korean Medicine, Semyung University)
  • 김호현 (세명대학교 한의과대학 생리학교실) ;
  • 안상현 (세명대학교 한의과대학 해부학교실) ;
  • 박선영 (세명대학교 한의과대학 생리학교실)
  • Received : 2018.12.31
  • Accepted : 2019.02.20
  • Published : 2019.02.25

Abstract

In order to investigate the effects of Eucomiae Cortex extracts on the depression caused by aging, histochemistry and immunohistochemistry were performed on the hippocampus of aged rats and the following results were obtained. Experimental animals were divided into three groups as follows: 8 week old ICR male mice, Aging-elicited group (AE group) and Eucomiae Cortex treatment group (EC group) 50 week old male ICR mice were used. The control group and AE group did not take any treatment and did not restrict diets and negatives. In the EC group, 0.51g/kg of Eucomiae Cortex extract was dissolved in distilled water once a day for 6 months. The Eucomiae Cortex extract reduced pyramidal neuronal damage in C3 hippocampus and dentate gyrus, increased DJ-1, SHH positive responses in aged mouse hippocampus, and 8-OHdG positivity was reduced, ${\beta}$-endorphin positivity was reduced in aged mouse substantia nigra. Therefore, based on the above results, Eucomiae Cortex extract reduces damage of pyramidal neurons in the hippocampus caused by aging, inhibits neuronal cell death, induces proliferation and differentiation of stem cells in the hippocampus, reduces DNA damage-induced oxidative stress, so improves the reduction of hippocampus volume. It is also thought to improves depression due to aging through ${\beta}$-endorphin which enhances mood through the inhibition of pain.

Keywords

References

  1. Kim HS, Kim BS. The effects of self-esteem on the relationship between the elderly stress and depression. Journal of the Korean Gerontological Society. 2007;27(1):23-37.
  2. Kwon SM, Min BB. Elderly mental disorders. Seoul: Hakjisa; 2000. p. 135, 199-201.
  3. Kim MH, Chung SD, Lee GY. Development and evaluation of preventive mental health program for depression among the elderly in community. Korean Journal of Social Welfare. 2001;44:318-45.
  4. Jung KH, Oh YH, Kang EN, Kim KR, Lee YK, Oh ME, et al. Survey of the elderly in 2017. Policy Report of Korea Institute for Health and Social Affairs. 2017.
  5. Ji YS. Clinical Exercise Prescription Mechanism. Ulsan: 21st century educational company; 2006.
  6. Hong K. Sports Medicine. Seoul: HongKyung publishing co.; 1998.
  7. Atchley RC. Social forces and aging. California: Wadsworth Publishing Company; 1991.
  8. Jeong HS, Yim HW, Jo SJ. Strategies to increase treatment adherence in elderly depression. Korean Journal of Research in Gerontology. 2013;22:79-93.
  9. Lorenzetti V, Allen NB, Fornito A, Yucel M. Structural brain abnormalities in major depressive disorder: a selective review of recent MRI studies. J Affect Disord. 2009;117(1-2):1-17. https://doi.org/10.1016/j.jad.2008.11.021
  10. Kim NY. Predicting factors of suicidal ideation in elderly with depression: focusing on social networks and resilience. The graduate school of information in clinical nursing, Hanyang University. 2019.
  11. Kim KR, Yoon JH. Effects of exercise training types on health related fitness, endocrine system in elderly women. Journal of Sport and Leisure Studies. 2005;25:263-75. https://doi.org/10.51979/KSSLS.2005.11.25.263
  12. Lee HM, Seo JC, Kim YS. The study on aging process research. Journal of Korean Acupuncture & Moxibustion Society 2001;18(1):146-56.
  13. Kim HC. Chinese medicine pharmacology. Seoul: Jipmoondang; 2001. p. 447-9.
  14. Lee SI, Ahn DK, Shin MK. Clinical application of Chinese medicine. Seoul: Seonbosa; 1982. p. 345-6.
  15. Wui OB. Sinnongbonchogyeong. Seoul: Eui Seong Dang Publishing Co.; 2003. p. 100-1.
  16. Kim SH, Kim IC. Antioxidative properties and whitening effects of the eucommiae cortex, salviae miltiorrhizae radix, aurantii nobilis pericarpium and cnidii rhizoma. J East Asian Soc Dietary Life 2008;18(4):618-23.
  17. Lee DS, Byun SY. Effects of the dietary mixture of eucommia ulmoides oliver on osteoporosis. Korean J Biotechnol. Bioeng. 2001;16(6):614-9.
  18. Hong EJ. Eucommia ulmoides extract stimulates glucose uptake through PI 3-kinase mediated pathway in L6 rat skeletal muscle cells. Graduate School of East-West medical science Kyunghee University: Dept. of East-west Medical Science. 2008.
  19. Choi YH, Seo JH, Kim JS, Heor JH, Kim SK, Choi SU, et al. Inhibitory effects of the stem bark extract of eucommia ulmoides on the proliferation of human tumor cell lines. Kor J Pharmacogn. 2003;34(4):308-13.
  20. Lee IS. Effects of eucommiae cortex administration on short-term memory, cell proliferation, and cell death in the hippocampus of aged rats. Graduate School of Kyungwon University: Dept. of oriental medicine. 2009.
  21. Rogers J, Webster S, Lue LF, Brachova L, Civin WH, Emmerling M, et al. Inflammation and Alzheimer's disease pathogenesis. Neurobiol Aging. 1996 Sep-Oct;17(5):681-6. https://doi.org/10.1016/0197-4580(96)00115-7
  22. Patrylo PR, Williamson A. The effects of aging on dentate circuitry and function. Prog Brain Res. 2007;163:679-96. https://doi.org/10.1016/S0079-6123(07)63037-4
  23. Is M, Comunoglu NU, Comunoglu C, Eren B, Ekici ID, Ozkan F. Age-related changes in the rat hippocampus. J Clin Neurosci. 2008 May;15(5):568-74. https://doi.org/10.1016/j.jocn.2007.03.025
  24. Wolfer DP, Lipp HP. Evidence for physiological growth of hippocampal mossy fiber collaterals in the guinea pig during puberty and adulthood. Hippocampus. 1995;5(4):329-40. https://doi.org/10.1002/hipo.450050406
  25. Gould E, Beylin A, Tanapat P, Reeves A, Shors TJ. Learning enhances adult neurogenesis in the hippocampal formation. Nat Neurosci. 1999 Mar;2(3):260-5. https://doi.org/10.1038/6365
  26. Eriksson PS, Perfilieva E, Bjork-Eriksson T, Alborn AM, Nordborg C, Peterson DA, et al. Neurogenesis in the adult human hippocampus. Nat Med. 1998 Nov;4(11):1313-7. https://doi.org/10.1038/3305
  27. Kim YP, Kim H, Shin MS, Chang HK, Jang MH, Shin MC, et al. Age-dependence of the effect of treadmill exercise on cell proliferation in the dentate gyrus of rats. Neurosci Lett. 2004 Jan 23;355(1-2):152-4. https://doi.org/10.1016/j.neulet.2003.11.005
  28. Kuhn HG, Dickinson-Anson H, Gage FH. Neurogenesis in the dentate gyrus of the adult rat: age-related decrease of neuronal progenitor proliferation. J Neurosci. 1996 Mar 15;16(6):2027-33. https://doi.org/10.1523/JNEUROSCI.16-06-02027.1996
  29. McKinnon MC, Yucel K, Nazarov A, MacQueen GM. A meta-analysis examining clinical predictors of hippocampal volume in patients with major depressive disorder. J Psychiatry Neurosci. 2009:34(1);41-54.
  30. Lee MS, Kwak DI, Jung IK. Impairment of self-maintenance skills and instrumental activities of daily life in geriatric depression. J Korean Geriatr Psythiatry. 2000;4(1):91-100.
  31. Lee JG, Kim EH, Lee JH. Effect of Gentianae Radix on Neurogenesis and Apoptosis in Hippocampus of Ethanol-induced Newborn Rats. J orient Neuropsychatr. 2010;21(2):29-44.
  32. Hampel H, Burger K, Teipel SJ, Bokde AL, Zetterberg H, Blennow K. Core candidate neurochemical and imaging biomarkers of Alzheimer's disease. Alzheimers Dement. 2008;4(1):38-48. https://doi.org/10.1016/j.jalz.2007.08.006
  33. Sousa N, Lukoyanov NV, Madeira MD, Almeida OF, Paula-Barbosa MM. Reorganization of the morphology of hippocampal neurites and synapses after stress-induced damage correlates with behavioral improvement. Neuroscience. 2000;97(2):253-66. https://doi.org/10.1016/S0306-4522(00)00050-6
  34. Girotto S, Cendron L, Bisaglia M, Tessari I, Mammi S, Zanotti G, et al. DJ-1 is a copper chaperone acting on SOD1 activation. J Biol Chem. 2014;289(15):10887-99. https://doi.org/10.1074/jbc.M113.535112
  35. Ingham PW, McMahon AP. Hedgehog signaling in animal development: paradigms and principles. Genes Dev. 2001;15(23):3059-87. https://doi.org/10.1101/gad.938601
  36. Pasca di Magliano M, Hebrok M. Hedgehog signalling in cancer formation and maintenance. Nat Rev Cancer. 2003;3(12):903-11. https://doi.org/10.1038/nrc1229
  37. Harman D. The aging process. Proc Natl Acad Sci U S A. 1981 Nov;78(11):7124-8. https://doi.org/10.1073/pnas.78.11.7124
  38. Harman D, Eddy DE, Noffsinger J. Free radical theory of aging: inhibition of amyloidosis in mice by antioxidants; possible mechanism. J Am Geriatr Soc. 1976 May;24(5):203-10. https://doi.org/10.1111/j.1532-5415.1976.tb06780.x
  39. Halliwell B, Chirico S. Lipid peroxidation: its mechanism, measurement, and significance. Am J Clin Nutr. 1993 May;57(5 Suppl):715S-724S https://doi.org/10.1093/ajcn/57.5.715S
  40. Gohil K, Packer L, de Lumen B, Brooks GA, Terblanche SE. Vitamin E deficiency and vitamin C supplements: exercise and mitochondrial oxidation. J Appl Physiol (1985). 1986 Jun;60(6):1986-91. https://doi.org/10.1152/jappl.1986.60.6.1986
  41. Hughes J, Smith TW, Kosterlitz HW, Fothergill LA, Morgan BA, Morris HR. Identification of two related pentapeptides from the brain with potent opiate agonist activity. Nature. 1975 Dec 18;258(5536):577-80. https://doi.org/10.1038/258577a0