Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Additive Anti-photoaging Effect of the ZnO Microsphere Soaked with Esculetin

Esculetin을 담지한 ZnO microsphere 외용제의 광노화 차단 상가 효능

  • Lee, Chang Hyun (Department of Anatomy, College of Korean Medicine, Woosuk University) ;
  • Hahn, Yoon Bong (School of Semiconductor and Chemical Engineering, Nanomaterials Processing Research Center, Chonbuk National University) ;
  • Jeong, Han-Sol (School of Korean Medicine, Division of Applied Medicine, Pusan National University)
  • 이창현 (우석대학교 한의과대학 해부학교실) ;
  • 한윤봉 (전북대학교 반도체화학공학부) ;
  • 정한솔 (부산대학교 한의학전문대학원 응용의학부)
  • Received : 2019.02.01
  • Accepted : 2019.02.25
  • Published : 2019.02.25
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Abstract

This study was aimed to examine the synergetic effects of photochemopreventive external agents composed of inorganic ZnO and esculetin. Zinc oxide (ZnO) is widely used in sunblocks because of its excellent biostability and little toxicity. Esculetin derived from Citrus Peel has an anti-oxidative effect. We made the hollow-shaped ZnO microsphere (MS), soaked it with esculetin (EZnO). We used SKH-1 mice to measure the photodamaging effects of UVB. The mice were divided into five groups as follows; UVB nontreated group (N), vehicle (C), esculetin (E), ZnO MS (ZnO), esculetin + ZnO MS(EZno) group. Each group of samples was topically applied to the dorsal skins before the UVB irradiation. The changes of collagen fibers in the skin tissues were observed by H & E staining and Van Gieson staining. The expression of mast cells in skin tissue was observed by immunohistochemical staining of tryptase present in the mast cell granules. Expression of inflammatory cytokines such as TNF-${\alpha}$, IL-$1{\beta}$, and MMP-9, which plays an essential role in wrinkle formation, was measured by RT-PCR. Interestingly, the composition of collagen fibers was better in the EZnO applied group than in the E or ZnO group. Moreover, mast cell expression and the expression of TNF-${\alpha}$, IL-$1{\beta}$, and MMP-9 mRNA were markedly suppressed in the EZnO group, indicating that the synergetic effects of esculetin and ZnO were excellent.

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References

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