DOI QR코드

DOI QR Code

Long non-coding RNA T-cell leukemia/lymphoma 6 serves as a sponge for miR-21 modulating the cell proliferation of retinoblastoma through PTEN

  • Tao, Sisi (Department of Science and Education, Changsha Hospital for Maternal & Child Health Care) ;
  • Wang, Weidong (Department of Science and Education, Changsha Hospital for Maternal & Child Health Care) ;
  • Liu, Pengfei (Department of Orthopedics, Shaoyang County People's Hospital) ;
  • Wang, Hua (Department of Ophthalmology, The First Affiliated Hospital of Human Normal University/Hunan Provincial People's Hospital) ;
  • Chen, Weirong (Health Management Center, Changsha Hospital for Maternal & Child Health Care)
  • 투고 : 2018.08.11
  • 심사 : 2019.04.15
  • 발행 : 2019.11.01

초록

Retinoblastoma (Rb) is one of the most common eye malignancies occur in childhood. The crucial roles of non-coding RNAs, particularly long non-coding RNAs (lncRNAs) and microRNAs (miRNAs), have been widely reported in Rb progression. In the present study, we found the expression of lncRNA T-cell leukemia/lymphoma 6 (TCL6) was significantly downregulated in Rb tissues and cell lines. Knockdown of lncRNA TCL6 promoted cell proliferation while reduced cell apoptosis in Rb cells. Moreover, lncRNA TCL6 serves as a sponge for miR-21, a previously-reported oncogenic miRNA in Rb, by direct targeting to negatively regulated miR-21 expression, therefore modulating Rb proliferation through miR-21. TCL6 overexpression inhibited Rb cell proliferation while miR-21 overexpression exerted an opposing effect; the effect of TCL6 overexpression was partially attenuated by miR-21 overexpression. PTEN/PI3K/AKT signaling pathway was involved in lncRNA TCL6/miR-21 axis modulating Rb cell proliferation. Taken together, lncRNA TCL6 serves as a tumor suppressor by acting as a sponge for miR-21 to counteract miR-21-mediated PTEN repression.

키워드

참고문헌

  1. Golabchi K, Soleimani-Jelodar R, Aghadoost N, Momeni F, Moridikia A, Nahand JS, Masoudifar A, Razmjoo H, Mirzaei H. MicroRNAs in retinoblastoma: potential diagnostic and therapeutic biomarkers. J Cell Physiol. 2018;233:3016-3023. https://doi.org/10.1002/jcp.26070
  2. Perez-Ramirez C, Canadas-Garre M, Molina MA, Faus-Dader MJ, Calleja-Hernandez MA. PTEN and PI3K/AKT in non-small-cell lung cancer. Pharmacogenomics. 2015;16:1843-1862. https://doi.org/10.2217/pgs.15.122
  3. Chen H, Zhou L, Wu X, Li R, Wen J, Sha J, Wen X. The PI3K/AKT pathway in the pathogenesis of prostate cancer. Front Biosci (Landmark Ed). 2016;21:1084-1091. https://doi.org/10.2741/4443
  4. Gui F, Hong Z, You Z, Wu H, Zhang Y. MiR-21 inhibitor suppressed the progression of retinoblastoma via the modulation of PTEN/PI3K/AKT pathway. Cell Biol Int. 2016;40:1294-1302. https://doi.org/10.1002/cbin.10678
  5. Wei D, Miao Y, Yu L, Wang D, Wang Y. Downregulation of microRNA-198 suppresses cell proliferation and invasion in retinoblastoma by directly targeting PTEN. Mol Med Rep. 2018;18:595-602.
  6. Zou WW, Xu SP. Galangin inhibits the cell progression and induces cell apoptosis through activating PTEN and Caspase-3 pathways in retinoblastoma. Biomed Pharmacother. 2018;97:851-863. https://doi.org/10.1016/j.biopha.2017.09.144
  7. Xie C, Lu H, Nomura A, Hanse EA, Forster CL, Parker JB, Linden MA, Karasch C, Hallstrom TC. Co-deleting Pten with Rb in retinal progenitor cells in mice results in fully penetrant bilateral retinoblastomas. Mol Cancer. 2015;14:93. https://doi.org/10.1186/s12943-015-0360-y
  8. Su S, Gao J, Wang T, Wang J, Li H, Wang Z. Long non-coding RNA BANCR regulates growth and metastasis and is associated with poor prognosis in retinoblastoma. Tumour Biol. 2015;36:7205-7211. https://doi.org/10.1007/s13277-015-3413-3
  9. Hao F, Mou Y, Zhang L, Wang S, Yang Y. LncRNA AFAP1-AS1 is a prognostic biomarker and serves as oncogenic role in retinoblastoma. Biosci Rep. 2018;38:BSR20180384. https://doi.org/10.1042/BSR20180384
  10. Volinia S, Calin GA, Liu CG, Ambs S, Cimmino A, Petrocca F, Visone R, Iorio M, Roldo C, Ferracin M, Prueitt RL, Yanaihara N, Lanza G, Scarpa A, Vecchione A, Negrini M, Harris CC, Croce CM. A microRNA expression signature of human solid tumors defines cancer gene targets. Proc Natl Acad Sci U S A. 2006;103:2257-2261. https://doi.org/10.1073/pnas.0510565103
  11. Abtin M, Alivand MR, Khaniani MS, Bastami M, Zaeifizadeh M, Derakhshan SM. Simultaneous downregulation of miR-21 and miR-155 through oleuropein for breast cancer prevention and therapy. J Cell Biochem. 2018;119:7151-7165. https://doi.org/10.1002/jcb.26754
  12. Markou A, Zavridou M, Lianidou ES. miRNA-21 as a novel therapeutic target in lung cancer. Lung Cancer (Auckl). 2016;7:19-27.
  13. LArki P, Ahadi A, Zare A, Tarighi S, Zaheri M, Souri M, Zali MR, Ghaedi H, Omrani MD. Up-regulation of miR-21, miR-25, miR-93, and miR-106b in gastric cancer. Iran Biomed J. 2018;22:367-373. https://doi.org/10.29252/.22.6.367
  14. Shen F, Mo MH, Chen L, An S, Tan X, Fu Y, Rezaei K, Wang Z, Zhang L, Fu SW. MicroRNA-21 down-regulates Rb1 expression by targeting PDCD4 in retinoblastoma. J Cancer. 2014;5:804-812. https://doi.org/10.7150/jca.10456
  15. Yamamura S, Imai-Sumida M, Tanaka Y, Dahiya R. Interaction and cross-talk between non-coding RNAs. Cell Mol Life Sci. 2018;75:467-484. https://doi.org/10.1007/s00018-017-2626-6
  16. Yang FY, Wang Y, Wu JG, Song SL, Huang G, Xi WM, Tan LL, Wang J, Cao Q. Analysis of long non-coding RNA expression profiles in clear cell renal cell carcinoma. Oncol Lett. 2017;14:2757-2764. https://doi.org/10.3892/ol.2017.6563
  17. Su H, Sun T, Wang H, Shi G, Zhang H, Sun F, Ye D. Decreased TCL6 expression is associated with poor prognosis in patients with clear cell renal cell carcinoma. Oncotarget. 2017;8:5789-5799. https://doi.org/10.18632/oncotarget.11011
  18. Maruyama R, Suzuki H. Long noncoding RNA involvement in cancer. BMB Rep. 2012;45:604-611. https://doi.org/10.5483/BMBRep.2012.45.11.227
  19. Gutschner T, Diederichs S. The hallmarks of cancer: a long noncoding RNA point of view. RNA Biol. 2012;9:703-719. https://doi.org/10.4161/rna.20481
  20. Shang W, Yang Y, Zhang J, Wu Q. Long noncoding RNA BDNF-AS is a potential biomarker and regulates cancer development in human retinoblastoma. Biochem Biophys Res Commun. 2018;497:1142-1148. https://doi.org/10.1016/j.bbrc.2017.01.134
  21. Zhang A, Shang W, Nie Q, Li T, Li S. Long non-coding RNA H19 suppresses retinoblastoma progression via counteracting miR-17-92 cluster. J Cell Biochem. 2018;119:3497-3509. https://doi.org/10.1002/jcb.26521
  22. Paramio JM, Navarro M, Segrelles C, Gomez-Casero E, Jorcano JL. PTEN tumour suppressor is linked to the cell cycle control through the retinoblastoma protein. Oncogene. 1999;18:7462-7468. https://doi.org/10.1038/sj.onc.1203151

피인용 문헌

  1. Expression and clinical significance of lncRNA TCL6 in serum of patients with preeclampsia vol.23, pp.1, 2019, https://doi.org/10.3892/etm.2021.10963