The Korean Journal of Physiology and Pharmacology

The Korean Society of Pharmacology (대한약리학회)
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Effects of rosmarinic acid on immunoregulatory activity and hepatocellular carcinoma cell apoptosis in H22 tumor-bearing mice

  • Cao, Wen (Department of Pharmacy, Guangxi International Zhuang Medicine Hospital) ;
  • Mo, Kai (Department of Pharmacy, Nanning First People's Hospital) ;
  • Wei, Sijun (Department of Pharmacy, Guangxi International Zhuang Medicine Hospital) ;
  • Lan, Xiaobu (Department of Pharmacy, Nanning First People's Hospital) ;
  • Zhang, Wenjuan (Department of Pharmacy, Guangxi International Zhuang Medicine Hospital) ;
  • Jiang, Weizhe (Department of Pharmacology, Guangxi Medical University)
  • Received : 2019.07.26
  • Accepted : 2019.09.27
  • Published : 2019.11.01
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Abstract

Rosmarinic acid (RA) is a natural polyphenolic compound that exists in many medicinal species of Boraginaceae and Lamiaceae. The previous studies have revealed that RA had therapeutic effects on hepatocellular carcinoma (HCC) in the H22-xenograft models by inhibiting the inflammatory cytokines and $NF-{\kappa}B$ p65 pathway in the tumor microenvironment. However, its molecular mechanisms of immunoregulation and pro-apoptotic effect in HCC have not been fully explored. In the present study, RA at 75, 150, and 300 mg/kg was given to H22 tumor-bearing mice via gavage once a day for 10 days. The results showed that RA can effectively inhibit the tumor growth through regulating the ratio of $CD4^+/CD8^+$ and the secretion of interleukin (IL)-2 and interferon-${\gamma}$, inhibiting the expressions of IL-6, IL-10 and signal transducer and activator of transcription 3, thereby up-regulating Bax and Caspase-3 and down-regulating Bcl-2. The underlying mechanisms involved regulation of immune response and induction of HCC cell apoptosis. These results may provide a more comprehensive perspective to clarify the anti-tumor mechanism of RA in HCC.

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References

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