The Korean Journal of Physiology and Pharmacology

  • 제24권1호
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  • Pages.101-110
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  • 2020
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  • 1226-4512(pISSN)
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  • 2093-3827(eISSN)
대한약리학회 (The Korean Society of Pharmacology)
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Analysis of interaction between intracellular spermine and transient receptor potential canonical 4 channel: multiple candidate sites of negatively charged amino acids for the inward rectification of transient receptor potential canonical 4

  • Kim, Jinsung (Department of Physiology, College of Medicine, Seoul National University) ;
  • Moon, Sang Hui (Office of Medical Education, College of Medicine, Seoul National University) ;
  • Kim, Taewook (Department of Physiology, College of Medicine, Seoul National University) ;
  • Ko, Juyeon (Department of Physiology, College of Medicine, Seoul National University) ;
  • Jeon, Young Keul (Department of Physiology, College of Medicine, Seoul National University) ;
  • Shin, Young-Cheul (Department of Cell Biology, Harvard Medical School) ;
  • Jeon, Ju-Hong (Department of Physiology, College of Medicine, Seoul National University) ;
  • So, Insuk (Department of Physiology, College of Medicine, Seoul National University)
  • 투고 : 2019.09.28
  • 심사 : 2019.10.16
  • 발행 : 2020.01.01
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초록

Transient receptor potential canonical 4 (TRPC4) channel is a nonselective calcium-permeable cation channels. In intestinal smooth muscle cells, TRPC4 currents contribute more than 80% to muscarinic cationic current (mIcat). With its inward-rectifying current-voltage relationship and high calcium permeability, TRPC4 channels permit calcium influx once the channel is opened by muscarinic receptor stimulation. Polyamines are known to inhibit nonselective cation channels that mediate the generation of mIcat. Moreover, it is reported that TRPC4 channels are blocked by the intracellular spermine through electrostatic interaction with glutamate residues (E728, E729). Here, we investigated the correlation between the magnitude of channel inactivation by spermine and the magnitude of channel conductance. We also found additional spermine binding sites in TRPC4. We evaluated channel activity with electrophysiological recordings and revalidated structural significance based on Cryo-EM structure, which was resolved recently. We found that there is no correlation between magnitude of inhibitory action of spermine and magnitude of maximum current of the channel. In intracellular region, TRPC4 attracts spermine at channel periphery by reducing access resistance, and acidic residues contribute to blocking action of intracellular spermine; channel periphery, E649; cytosolic space, D629, D649, and E687.

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참고문헌

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