Journal of Forest and Environmental Science

  • 제36권4호
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  • Pages.274-280
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  • 2020
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  • 2288-9744(pISSN)
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  • 2288-9752(eISSN)
강원대학교 산림과학연구소 (Institute of Forest Science, kangwon National University)
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Optimization Conditions for Cryopreservation of Deutzia paniculata Nakai, Endangered Plant

  • Seol, Yuwon (Division of Environmental Forest Science, Gyeongsang National University (Institute of Agriculture of Life Science)) ;
  • Yong, Seong Hyeon (Division of Environmental Forest Science, Gyeongsang National University (Institute of Agriculture of Life Science)) ;
  • Choi, Eunji (Division of Environmental Forest Science, Gyeongsang National University (Institute of Agriculture of Life Science)) ;
  • Jeong, Mi Jin (Division of Plant Resources, Korea National Arboretum) ;
  • Suh, Gang Uk (Division of Plant Resources, Korea National Arboretum) ;
  • Lee, Cheul Ho (DMZ Botanic Garden, Korea National Arboretum) ;
  • Choi, Myung Suk (Division of Environmental Forest Science, Gyeongsang National University (Institute of Agriculture of Life Science))
  • 투고 : 2020.04.20
  • 심사 : 2020.07.15
  • 발행 : 2020.12.31
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초록

As the importance of biological resources increases, the conservation technology is becoming important for rarities. This study was conducted to establish an efficient cryopreservation conditions for the Deutzia paniculata, endangered plant species, by using both cryopreservation methods of vitrification and encapsulation. As a result, the sucrose pretreatment seed viability showed up to 30.7% in the treatments. The cryoprotectant treatment improved the viability of the seeds, and was found to be excellent in the vitrification method using PVS3. The vitrification method had over 10% higher germination rate than the seeds preserved by encapsulation. In addition, the germination rate showed a significant difference according to the cryopreservation treatment time, and the germination rate of seeds decreased very much as the long time became longer. Plants germinated from preserved seed in liquid nitrogen showed poor growth compared to untreated, and good growth in PVS3 120 minutes. In addition, the growth of germinated plants by liquid nitrogen treatment time was better in the vitrification method. These results are expected to be useful for long-term preservation of D. paniculata, endangered plants.

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