Journal of Mushroom (한국버섯학회지)

The Korean Society of Mushroom Science (한국버섯학회)
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Unrecorded fungi isolated from Lentinula edodes cultivation houses in Korea

표고 재배사에서 분리한 국내 미기록 진균 보고

  • Ahn, Geum Ran (Department of Microbiology, Dankook University) ;
  • Noh, Hyeungjin (Department of Microbiology, Dankook University) ;
  • Kim, Jun Young (Department of Microbiology, Dankook University) ;
  • Ko, Han Gyu (Forest Mushroom Research Center, National Forest Cooperative Federation) ;
  • Kim, Seong Hwan (Department of Microbiology, Dankook University)
  • 안금란 (단국대학교 미생물학과) ;
  • 노형진 (단국대학교 미생물학과) ;
  • 김준영 (단국대학교 미생물학과) ;
  • 고한규 (산림조합중앙회 산림버섯연구센터) ;
  • 김성환 (단국대학교 미생물학과)
  • Received : 2019.12.16
  • Accepted : 2020.03.04
  • Published : 2020.03.31
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Abstract

During the monitoring of fungal pests in 2016 and 2017, Acrodontium crateriforme, Naganishia friedmannii, Pestalotiopsis trachicarpicola, Penicillium wollemiicola, and Trichoderma thailandicum were isolated from indoor air, mushroom flies (Phytosciara flavipes), and media materials in the cultivation houses of oak wood mushroom (Lentinula edodes) located in Seocheon, Jangheung, Buyeo, and Yeoju, Korea. These fungal species were identified based on their morphological characteristics after their growth on PDA and subsequent molecular analyses of the 26S rDNA, 28S rDNA, β-tubulin gene, and translation elongation factor 1-α gene using PCR amplification and nucleotide sequencing were performed. The results showed that these fungi were previously undocumented in Korea. This study reports descriptions of their taxonomical and known properties.

2016년과 2017년 서천, 장흥, 부여, 여주에 소재하는 표고 재배사에서 진균성 병원균을 모니터링 하는 과정에서 Acrodontium crateriforme, Naganishia friedmannii, Pestalotiopsis trachicarpicola, Penicillium wollemiicola, Trichoderma thailandicum 등 5 종의 국내 미기록 진균을 재배사내 실내 공기, 버섯 파리, 버섯 배지재료 등에서 분리하였다. 이들 진균은 PDA 배양하여 생장시키면서 형태적 특성 조사하고 26S rDNA, 28S rDNA, β-TUB 유전자, TEF 유전자 등을 PCR 기술로 증폭하고 그 염기서열 분석을 수행하여 동정하였다. 본 논문에서는 이들의 분류학적 위치와 위해성에 대해 보고하고자 한다.

Keywords

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