Journal of Mushroom (한국버섯학회지)

The Korean Society of Mushroom Science (한국버섯학회)
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Cultivation characteristics and genetic diversity of wild-type collections of Hericium erinaceus in Korea

국내 노루궁뎅이버섯 야생수집균주의 자실체 특성 및 유전적 다양성

  • Park, Sun-Ae (Soloom Biotech) ;
  • Lim, Sun-Hwa (Department of Horticultural Biotechnology, Hankyong National University) ;
  • Kim, Ja-Yoon (Department of Horticultural Biotechnology, Hankyong National University) ;
  • Kang, Hee-Wan (Department of Horticultural Biotechnology, Hankyong National University)
  • Received : 2020.02.14
  • Accepted : 2020.03.05
  • Published : 2020.03.31
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Abstract

Wild-type isolates of H. erinaceus were collected from different geographical areas of Korea. Nineteen isolates were cultured on mushroom substrate for producing fruiting bodies. Of these, 14 isolates formed pinheads and fruiting bodies on the substrate. The morphological and cultivation characteristics of fruiting bodies were categorized by pinheading, fruit body formation, spine types, fresh weights, and colors. Microstructures, including spines, spores, and basidia on the fruiting bodies were observed using scanning electron microscopy. The H. erinaceus isolates demonstrated different PCR polymorphisms produced by universal fungal primers (UFPs) and were classified into four groups based on their high genetic diversity.

국내의 다양한 지역에서 수집한 19 야생버섯균주를 이용하여 배지조성인 참나무 톱밥과 밀기울을 부피비율 7:3으로 병재배 650 g/1,100 ml에서 자실체를 생산하였다, 시험한 19균주중 14균주가 원기를 형성하였으며 자실체를 생산 할 수 있었으며 대조품종 노루1, 2호의 병당 75 g보다 KFRI349, KFRI1091, KFRI1093은 병당 120 g 이상으로 높은 수확량을 나타내었다. 자실체 색형은 백색과 핑크색으로 나눌 수 있었으며 침의 형태에 따라 고드름형과 산호형으로 구분할 수 있었다. 전자현미경(SEM)에 의한 노루궁뎅이버섯의 담자기, 포자, 침의 형태를 포함한 자실체 미세구조를 관찰 하였으며 UFP-PCR분석으로 균주간의 PCR다형성밴드가 검출되어 4 그룹으로 분류 할 수 있었으며 높은 유전적 다양성을 확인 할 수 있었다.

Keywords

References

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