Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Studies on Anti-inflammatory Effects of Mahwanghangingamchosukgo-tang Extracts

마황행인감초석고탕 추출물의 항염증 효과에 대한 연구

  • Park, Jung Eun (School of Cosmetic Science and Beauty Biotechnology, Semyung University) ;
  • Hwang, Hyung Seo (School of Cosmetic Science and Beauty Biotechnology, Semyung University) ;
  • Kim, Tae Yeon (College of Korean Medicine, Semyung University)
  • 박정은 (세명대학교 화장품뷰티생명공학부) ;
  • 황형서 (세명대학교 화장품뷰티생명공학부) ;
  • 김태연 (세명대학교 한의과대학)
  • Received : 2020.10.08
  • Accepted : 2020.12.23
  • Published : 2020.12.25
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Abstract

Mahwanghangingamchosukgo-tang (MH) is recorded as a treatment to treat exterior-related respiratory diseases in the Korean medicine. In this study, we examined the anti-inflammatory effects of MH, using MH water extract and lipopolysaccharide (LPS)-induced RAW 264.7 cells. First of all, we measured the amount of nitric oxide (NO) and prostaglandin E2 (PGE2), the products of inflammatory metabolism. Also, we measured enzymes such as inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), as well as cytokines such as interleukin 6 (IL-6), interleukin 1 alpha (IL-1α), and interleukin 1 beta (IL-1β). MH suppressed the production of NO and PGE2 in a dose dependent manner and reduced the amount of protein and the mRNA expression of iNOS and COX-2. Also, MH reduced the mRNA expression of IL-6, IL-1α and IL-1β. In conclusion, MH decreased production of LPS-induced inflammatory factor, which could be a clinical basic subject for inflammatory diseases.

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References

  1. Vinay K, Abul A, Nelson F, Jon A. Robbins & Cotran Pathologic Basis of disease. 8th ed. Amsterdam:Elsevier Inc;2011. p. 69.
  2. Choi BG. Free radicals and diseases. Seoul:Shinil books;2004. p. 214.
  3. Textbook Compilation Committee of Pathology in Korean Medicine. Pathology in Korean Medicine. Yongin:Hanuimunhwasa;2019. p. 41-3, 64, 66, 440.
  4. Moon JJ, An GS, Kim JB, Park WS, Park JH, Kim DH et al. Precise Interpretation of 'Treatise on Cold Damage Diseases'. Yongin:Hanuimunhwasa;2014. p. 162-4, 338-9.
  5. National College of Korean Medicine Collaborative Textbook Compilation Committee. Herbology. Seoul:Younglim Publishing Inc;2000. p. 121, 160, 478, 540.
  6. Hong ND, Jeong GM, Moon JH, Joo SM. Effects of Mahaengkamsuk-tang on Analgesic, Antipyretic, Antiinflammatory, Secretion of Respiratory Tract, Isolated Ileum and Blood Pressure. Kor. J. Pharmacogn. 1986;17(3):223-32.
  7. Lee JH, Jung SG, Rhee HK. Effect of Mahwanghangingamchosukgo tang & Mahwanghangingamchosukgotang gamibang on the Pulmonary Function of SO2-Exposed Rats. K. H. Univ. O Med. J. 1993;16:85-105.
  8. Lee JG, Koo YS, Lee YG, Park YC. Effect of Mahaenggamseok-tang-gagambang on CD3, CD4, CD8 Cells in OVA-induced Asthmatic Mice. Journal of Oriental Medicine Research Institute in Daejeon University. 2008;17(1):67-74.
  9. Park GB, Park YC. Effects of Mahaenggamseok-tang-gagambang on Immune Cells and Cytokines in OVA-Induced Asthmatic Mice. Korean J. Oriental Physiology & Phathology. 2009;23(3):590-8.
  10. Park SH, Jeong HJ, Jeong SG, Lee HG. The Inhibitory Effects Mahaenggamseok-Tang and Platycodi Radix on the Cytokines of Human Epitherial Cells. K. H. M. 2001;17(2);230-41.
  11. Lee YB, Jeong AR. Mahaenggamsuktang for treating Mycoplasma pneumonia in Children: Systematic Review and Meta-analysis of Randomized Controlled Trials (RCTs). J Pediatr Korean Med. 2020;34(2):86-104.
  12. Jang JT, Seo IB, Kim JB. Effects of Gamimahaenggamseok-tang and Typhae Pollen on Body Weight and Lipid Metabolism of Rats with Obesity induced by High Fat Diets. Korean J. Oriental Physiology & Phathology. 2003;17(1):190-202.
  13. Cho JH. Effect of Mahaenggamseok-Tang(Mahuangjiazhu-tang) on Anti-Obesity in High Fat Diet-induced Obese Sprague-Dawley Rat. Naju:Dongshin University;2011. p. 42.
  14. Noh HM, Park SG, Kweon SH, Jo EH, Park MC. Case Report of the Patient with the Atopic Dermatitis, Psoriasis Treated with Mahaenggamseog-tang. 2018;32(1):80-7. https://doi.org/10.15188/kjopp.2018.02.32.1.80
  15. Textbook Compilation Committee of Herbal Pharmacology. Herbal Pharmacology. 3rd ed. Seoul:Sinil books;2010. p. 116-7, 182-4, 273-7, 328-30.
  16. Yeom MJ, Lee HC, Kim GH, Lee HJ, Shim IS, Oh SK, Kang SK, Hahm DH. Anti-arthritic Effects of Ephedra sinica STAPF Herb-Acupuncture: Inhibition of Lipopolysaccharide-Induced Inflammation and Adjuvant-Induced Polyarthritis. J Pharmacol Sci. 2006;100(1):41-50. https://doi.org/10.1254/jphs.FP0050637
  17. DO JS. Hwang JK, Seo HJ, Woo WH, Nam SY. Antiasthmatic activity and selective inhibition of type 2 helper T cell response by aqueous extract of semen armeniacae amarum. Immunopharmacol Immunotoxicol. 2006;28(2):213-38. https://doi.org/10.1080/08923970600815253
  18. Yang HY, Chang HK, Lee JW, Kim YS, Kim H, Lee MH, Shin HS, Ham DH, Park HK, Lee HJ, Kim CJ. Amygdalin suppresses lipopolysaccharide-induced expressions of cyclooxygenase-2 and inducible nitric oxide synthase in mouse BV2 microglial cells. Neurol Res. 2007;29(1):59-64. https://doi.org/10.1179/016164107X172248
  19. Zhu YP. Chinese Materia Medica Chemistry, Pharmacology and applications. The Netherlands: Harwood Academic Publishers;1998. p. 496-8.
  20. Fukai T, Satoh K, Nomura T, Sakagami H. Preliminary evaluation of antinephritis and radical scavenging activities of glabridin from Glycyrrhiza glabra. 2003;74(7):624-9. https://doi.org/10.1016/S0367-326X(03)00164-3
  21. Saeedi M, Morteza-Semnani K, Ghoreishi MR. The treatment of atopic dermatitis with licorice gel. 2003;14(3):153-60.
  22. Amagaya S, Sugishita E, Ogihara Y, Ogawa S, Okada K, Aizawa T. Comparative studies of the stereoisomers of glycyrrhetinic acid on anti-inflammatory activities. J Pharmacobiodyn. 1984;7(12):923-8. https://doi.org/10.1248/bpb1978.7.923
  23. Masayasu K. Herbal Pharmacology. Tokyo:Namsandang ;1997. p. 132-3.
  24. Kindt TJ, Goldsby RA, Osborne BA. Innate immunity : Tenney S. Kuby Immunology. 6th ed. New York:Freeman press;2007. p. 52-73.
  25. Kim BH, Lee YT, Kang KH. Codonopsis Lanceolata Inhibits Inflammation through Regulation of MAPK in LPS-stimulated RAW264.7 cells. J Physiol Pathol Korean Med. 2010;24(1):80-4.
  26. Dawson TM, Dawson VL, Snyder SH. A novel neuronal messenger molecule in brain:The free radical, nitric oxide. Ann Neurol. 1992;32:297-311. https://doi.org/10.1002/ana.410320302
  27. Wang MJ, Lin WW, Chen HL, Chang YH, Ou HC, Kuo JS, et al. Silymarin protects dopaminergic neurons against lipopolysaccharide-induced neurotoxicity by inhibiting microglia activation. Eur J Neurosci. 2002;16(11):2103-12. https://doi.org/10.1046/j.1460-9568.2002.02290.x
  28. Hanisch UK. Microglia as a source and target of cytokines. Glia. 2002;40(2):140-55. https://doi.org/10.1002/glia.10161
  29. Chen W, Tang Q, Gonzales MS, Bowden GT. Role of p38 MAP kinases and ERK in mediating ultraviolet-B induced cyclooxygenase-2 gene expression in human keratinocytes. Oncogene. 2001;20(29):3921-6. https://doi.org/10.1038/sj/onc/1204530
  30. Marina L, Kamal RM, Andrew F, Gary B, Jeremy S, Andrew RC. Regulation of Cyclooxygenase 2 mRNA Stability by the Mitogen-Activated Protein Kinase p38 Signaling Cascade. Mol Cell Biol. 2000;20(12):4265-74. https://doi.org/10.1128/MCB.20.12.4265-4274.2000
  31. Mitchell JA, Larkin S, Williams TJ. Cyclooxygenase-2 :regulation and relevance in inflammation. Biochemical Pharmacology. 1995;50(10):1535-42. https://doi.org/10.1016/0006-2952(95)00212-X
  32. Crofford LJ, Lipsky PE, Brooks P, Abramson SB, Simon LS, Van de Putte LB. Basic biology and clinical application of specific cyclooxygenase-2 inhibitors. Arthritis Rheum. 2000;43(1):4-13. https://doi.org/10.1002/1529-0131(200001)43:1<4::AID-ANR2>3.0.CO;2-V
  33. Raabe T, Bukrinsky M, Currie RA. Relative contribution of transcription and translation to the induction of tumor necrosis factor-alpha by lipopolysaccharide. J Biol Chem. 1998;273(2):974-80. https://doi.org/10.1074/jbc.273.2.974