DOI QR코드

DOI QR Code

Monitoring of Biotin Content in Frequently Consumed Foods in Korea

국내 다소비 식품의 biotin 함량 모니터링

  • Kwon, Jihyun (School of Food Biotechnology and Nutrition, Kyungsung University) ;
  • Cheon, Wonyoung (School of Food Biotechnology and Nutrition, Kyungsung University) ;
  • Lee, Sang-Hoon (Department Agrofood Resources, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Choi, Youngmin (Department Agrofood Resources, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Kim, Younghwa (School of Food Biotechnology and Nutrition, Kyungsung University)
  • 권지현 (경성대학교 식품응용공학부) ;
  • 천원영 (경성대학교 식품응용공학부) ;
  • 이상훈 (농촌진흥청 국립농업과학원 농식품자원부) ;
  • 최용민 (농촌진흥청 국립농업과학원 농식품자원부) ;
  • 김영화 (경성대학교 식품응용공학부)
  • Received : 2020.06.09
  • Accepted : 2020.06.22
  • Published : 2020.06.30

Abstract

In this study, biotin (vitamin B7) contents of frequently consumed foods in Korea were determined by using immunoaffinity column in conjunction with high-performance liquid chromatography (HPLC). The biotin contents of 24 foods of plant origin and 27 foods of animal origin were selected. The highest biotin contents in frequently consumed foods of plant origin were found in red beans (Huinguseul; 11.475 ㎍/100 g). On the other hand, biotin was not detected in any varieties of sorghum. For frequently consumed foods of animal origin, salted pollack roe (7.486 ㎍/100 g) showed the highest biotin content. However, beef and fish contained less biotin. All biotin analyses were conducted under analytical quality control. The limits of detection and limits of quantification of biotin were 0.007 and 0.023 ㎍/100 g, respectively, and the accuracy/recovery percentage was 95.35-105.02%. The precision values were 4.041% (repeatability) and 3.835% (reproducibility). Taken together, our data provide reliable data on the biotin contents of frequently consumed foods in Korea.

Keywords

References

  1. AOAC. AOAC guidelines for single laboratory validation of chemical methods for dietary supplements and botanicals. Association of Official Agricultural Chemists International, Gaithersburg, MD, USA. 2016
  2. Gill BD, Saldo S, Wood JE, Indyk HE. 2018. A rapid method for the determination of biotin and folic acid in liquid milk, milk powders, infant formula, and milk-based nutritional products by liquid chromatography-tandem mass spectrometry. J AOAC Int. 101(5):1578-1583 https://doi.org/10.5740/jaoacint.18-0065
  3. Holler U, Wachter F, Wehrli C, Fizet C. 2006. Quantification of biotin in feed, food, tablets, and premixes using HPLC-MS/MS. J Chromatogr B Analyt Technol Biomed Life Sci. 831(1-2):8-16 https://doi.org/10.1016/j.jchromb.2005.11.021
  4. Hwang IG, Byun JY, Kim KM, Jung MN, Yoo SM. 2014. Vitamin C quantification of Korea sweet potatoes by cultivar and cooking method. J Korean Soc Food Sci Nutr. 43(6):955-961 https://doi.org/10.3746/JKFN.2014.43.6.955
  5. Hwang JH, Kim JM. 2001. Physicochemical properties of commercial salt-fermented shrimp. J Korea Soc Food Sci Nutr. 30(4):760-763
  6. Joseph G, Devi R. 2018. Determination of total biotin by liquid chromatography coupled with immunoaffinity column cleanup extraction: multilaboratory testing, Final Action 2016.02. J AOAC Int. 101(3):831-842 https://doi.org/10.5740/jaoacint.17-0242
  7. Kergaravat SV, Gomez GA, Fabiano SN, Laube Chavez TI, Pividori MI, Hernandez SR. 2012. Biotin determination in food supplements by an electrochemical magneto biosensor. Talanta. 97:484-490 https://doi.org/10.1016/j.talanta.2012.05.003
  8. Kim DS, Lee YJ. Jeong DY, Lee DY, Ahn MK. 2003. Determination of biotin by HPLC. Anal Sci Technol. 16:499-503
  9. Kim JH, Hwang HW, Cho YJ, Park JW. 2020. A study on collecting representative food samples for the 10th Korean standard foods composition table. The Korean Journal of Applied Statistics. 33(2):215-228 https://doi.org/10.5351/KJAS.2020.33.2.215
  10. Kim JY, Oh CH. 2011. Analysis of biotin in Korean representative foods and dietary intake assessment for Korean. Food Sci Biotechnol. 20(4):1043-1049 https://doi.org/10.1007/s10068-011-0142-2
  11. Kwon JH, Lee SH, Choi Y, Kim Y. 2019. Biotin analysis in selected agricultural foods consumed in Korea. J Korea Soc Food Sci Nutr. 48(12):1366-1372 https://doi.org/10.3746/jkfn.2019.48.12.1366
  12. Lin Q, Ding Y, Poh F, Zhang C, Pan SJ, Schimpf KJ. 2017. Determination of biotin in infant, pediatric, and adult nutritionals by high-performance liquid chromatography and fluorescence detection: single-laboratory validation, First Action 2016.11. J AOAC Int. 100(1):145-151 https://doi.org/10.5740/jaoacint.16-0257
  13. Livaniou E, Costopoulou D, Vassiliadou I, Leondiadis L, Nyalala JO, Ithakissios DS, Evangelatos GP. 2000. Analytical techniques for determining biotin. J Chromatogr A. 881(1-2):331-343 https://doi.org/10.1016/S0021-9673(00)00118-7
  14. Luong JHT, Male KB, Glennon JD. 2019. Biotin interference in immunoassays based on biotin-strept (avidin) chemistry:An emerging threat. Biotechnol Adv. 37(5):634-641 https://doi.org/10.1016/j.biotechadv.2019.03.007
  15. Shin YW, Lee HJ, Ham HS, Shin SC, Kang YJ, Hwang KM, Kwon YK, Seo IW, Oh JM, Koo YE. 2016. Establishment of biotin analysis by LC-MS/MS method in infant milk formulas. J Food Hyg Saf. 31(5):327-334 https://doi.org/10.13103/JFHS.2016.31.5.327
  16. Watanabe T, Kioka M, Fukushima A, Morimoto M, Sawamura H. 2014. Biotin content table of select foods and biotin intake in Japanese. Int J Anal Bio Sci. 2(4):109-125
  17. Yomota C, Ohnishi Y. 2007. Determination of biotin following derivatization with 2-nitrophenylhydrazine by highperformance liquid chromatography with on-line UV detection and electrospray-ionization mass spectrometry. J Chromatogr A. 1142(2): 231-235 https://doi.org/10.1016/j.chroma.2006.12.058