Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Effect of Leonurus japonicus Houtt. on Scopolamine-induced Memory Impairment in Mice

Scopolamine 유발 기억 손상 마우스에서 익모초의 효과

  • Lee, Jihye (Department of Pharmacology, Daegu Haany University) ;
  • Kim, Hye-Jeong (Department of Pharmacology, Daegu Haany University) ;
  • Jang, Gwi Yeong (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Seo, Kyung Hye (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Kim, Mi Ryeo (Department of Herbal Pharmacology, Daegu Haany University) ;
  • Choi, Yun Hee (Department of Oriental Medicine & Healthcare, WonKwang Digital University) ;
  • Jung, Ji Wook (Department of Pharmacology, Daegu Haany University)
  • 이지혜 (대구한의대학교 약리학연구실) ;
  • 김혜정 (대구한의대학교 약리학연구실) ;
  • 장귀영 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 서경혜 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 김미려 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 최윤희 (원광디지털대학교 한방건강학과) ;
  • 정지욱 (대구한의대학교 약리학연구실)
  • Received : 2019.11.19
  • Accepted : 2020.02.25
  • Published : 2020.04.25
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Abstract

Cognitive impairment is symptoms of dementia, a degenerative brain disease that is drawing attention in a rapidly aging society. This study was conducted to investigate the improvement of cognitive function of Leonurus japonicus on scopolamine-induced memory impairment in mice and the effect and mechanism of memory recovery. In vivo studies were conducted on mice orally pretreated with L. japonicus in doses of 50, 100 and 200 mg/kg (p.o.) and scopolamine (1 mg/kg, i.p.) were injected 30 min before the behavioral task. Antioxidant activity was assessed by 2,2-diphenyl-1-picryl hydrazyl (DPPH) assay and 2,2-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assay, and acetylcholinesterase (AChE) inhibition activity evaluated by Ellman's method. In behavior studies showed that L. japonicus has an improved the memory of scopolamine-treated mice in Y-maze, passive avoidance and Morris water maze test. In addition, L. japonicus was also exerted free radical scavenging activity and inhibited acetyl cholinesterase activity. These results suggest that L. japonicus improves short-term and long-term memory in scopolamine-induced memory decline model and prevents scopolamine-induced memory impairments through in reduced oxidative stress and acetyl cholinesterase inhibition effect. Thus, L. japonicus is related to functional medicinal materials for prevention and treatment of human dementia patients.

Keywords

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