Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Effect of L-carnitine on sperm quality during liquid storage of boar semen

  • Yang, Kang (Department of Biotechnology, College of Life Science Northeast Agricultural University) ;
  • Wang, Na (Department of Biotechnology, College of Life Science Northeast Agricultural University) ;
  • Guo, Hai-Tao (Department of Biotechnology, College of Life Science Northeast Agricultural University) ;
  • Wang, Jing-Ran (Department of Biotechnology, College of Life Science Northeast Agricultural University) ;
  • Sun, Huan-Huan (Department of Biotechnology, College of Life Science Northeast Agricultural University) ;
  • Sun, Liang-Zhen (Department of Biotechnology, College of Life Science Northeast Agricultural University) ;
  • Yue, Shun-Li (Department of Biotechnology, College of Life Science Northeast Agricultural University) ;
  • Zhou, Jia-Bo (Department of Biotechnology, College of Life Science Northeast Agricultural University)
  • Received : 2019.06.01
  • Accepted : 2019.11.12
  • Published : 2020.11.01
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Abstract

Objective: This study was conducted to investigate the effect of L-carnitine on the pig semen characteristics during storage. Methods: Spermatozoa samples were examined for spermatozoa quality and then randomly divided into 5 groups: 0 (control), 12.5, 25, 50, and 100 mM L-carnitine. Sperm motility, plasma membrane integrity and antioxidant parameters (total reactive oxygen species, total antioxidant capacity, and malondialdehyde) were evaluated after 0, 3, 5, and 10 day cooled-storage at 17℃. Moreover, ATP content, mitochondria activity as well as sperm-binding and in vitro fertilizing ability of preserved boar sperm were also investigated. Results: Supplementation with 50 mM L-carnitine could effectively maintain boar sperm quality parameters such as sperm motility and membrane integrity. Besides, we found that L-carnitine had positive effects on boar sperm quality mainly through improving antioxidant capacities and enhancing ATP content and mitochondria activity. Interestingly, by assessing the effect of L-carnitine on sperm fertility and developmental potential, we discovered that the extender containing L-carnitine could improve sperm quality and increase the number of sperms bounding to zona pellucida, without improving in vitro fertility and development potential. Conclusion: These findings suggested that the proper addition of L-carnitine to the semen extender improved boar sperm quality during liquid storage at 17℃.

Keywords

References

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