Journal of Ginseng Research

The Korean Society of Ginseng (고려인삼학회)
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Novel enzymatic elimination method for the chromatographic purification of ginsenoside Rb3 in an isomeric mixture

  • Cui, Chang-Hao (The Key Laboratory of Biotechnology for Medicinal Plant of Jiangsu Province, Jiangsu Normal University) ;
  • Fu, Yaoyao (The Key Laboratory of Biotechnology for Medicinal Plant of Jiangsu Province, Jiangsu Normal University) ;
  • Jeon, Byeong-Min (Department of Biological Sciences, Korea Advanced Institute of Science and Technology) ;
  • Kim, Sun-Chang (Intelligent Synthetic Biology Center) ;
  • Im, Wan-Taek (Department of Biological Sciences, Hankyong National University)
  • Received : 2018.06.27
  • Accepted : 2019.08.12
  • Published : 2020.11.15
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Abstract

Background: The separation of isomeric compounds from a mixture is a recurring problem in chemistry and phytochemistry research. The purification of pharmacologically active ginsenoside Rb3 from ginseng extracts is limited by the co-existence of its isomer Rb2. The aim of the present study was to develop an enzymatic elimination-combined purification method to obtain pure Rb3 from a mixture of isomers. Methods: To isolate Rb3 from the isomeric mixture, a simple enzymatic selective elimination method was used. A ginsenoside-transforming glycoside hydrolase (Bgp2) was employed to selectively hydrolyze Rb2 into ginsenoside Rd. Ginsenoside Rb3 was then efficiently separated from the mixture using a traditional chromatographic method. Results: Chromatographic purification of Rb3 was achieved using this novel enzymatic elimination-combined method, with 58.6-times higher yield and 13.1% less time than those of the traditional chromatographic method, with a lower minimum column length for purification. The novelty of this study was the use of a recombinant glycosidase for the selective elimination of the isomer. The isolated ginsenoside Rb3 can be used in further pharmaceutical studies. Conclusions: Herein, we demonstrated a novel enzymatic elimination-combined purification method for the chromatographic purification of ginsenoside Rb3. This method can also be applied to purify other isomeric glycoconjugates in mixtures.

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References

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