Pediatric Gastroenterology, Hepatology & Nutrition

The Korean Society of Pediatric Gastroenterology, Hepatology and Nutrition (대한소아소화기영양학회)
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Evidence for Differentiation of Colon Tissue Microbiota in Patients with and without Postoperative Hirschsprung's Associated Enterocolitis: A Pilot Study

  • Arbizu, Ricardo A. (Division of Pediatric Gastroenterology, Department of Pediatrics, Medical University of South Carolina) ;
  • Collins, David (South Carolina Clinical and Translational Research Institute) ;
  • Wilson, Robert C. (Department of Pathology and Laboratory Medicine, Medical University of South Carolina) ;
  • Alekseyenko, Alexander V. (Program for Human Microbiome Research, Biomedical Informatics Center, Departments of Public Health Sciences in College of Medicine, Oral Health Sciences in College of Dental Medicine, and Healthcare Leadership and Management in College of Health Professions, Medical University of South Carolina)
  • Received : 2020.06.10
  • Accepted : 2020.08.17
  • Published : 2021.01.15
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Abstract

Purpose: To investigate the differences in the colon microbiota composition of Hirschsprung's disease (HSCR) patients with and without a history of postoperative Hirschsprung's associated enterocolitis (HAEC). Methods: Colon tissue microbiota was characterized by bacterial deoxyribonucleic acid (DNA) extraction and 16S rDNA sequencing for taxonomic classification and comparison. Results: The sequence diversity richness within samples was significantly higher in samples from patients with a history of postoperative HAEC. We observed an increased relative abundance of the phyla Bacteroidetes, Firmicutes and Cyanobacteria in HAEC patients and Fusobacteria, Actinobacteria and Proteobacteria in HSCR patients and, an increased relative abundance of the genera Dolosigranulum, Roseouria and Streptococcus in HAEC patients and Propionibacterium and Delftia in HSCR patients. Conclusion: Our findings provide evidence that the colon tissue microbiota composition is different in HSCR patients with and without postoperative HAEC.

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References

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  1. Alterations of Gut Bacteria in Hirschsprung Disease and Hirschsprung-Associated Enterocolitis vol.9, pp.11, 2021, https://doi.org/10.3390/microorganisms9112241