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Effects of lycopene on abdominal fat deposition, serum lipids levels and hepatic lipid metabolism-related enzymes in broiler chickens

  • Wan, Xiaoli (College of Animal Science and Technology, Yangzhou University) ;
  • Yang, Zhengfeng (College of Animal Science and Technology, Yangzhou University) ;
  • Ji, Haoran (College of Animal Science and Technology, Yangzhou University) ;
  • Li, Ning (College of Animal Science and Technology, Yangzhou University) ;
  • Yang, Zhi (Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Yangzhou University) ;
  • Xu, Lei (College of Animal Science and Technology, Yangzhou University) ;
  • Yang, Haiming (College of Animal Science and Technology, Yangzhou University) ;
  • Wang, Zhiyue (College of Animal Science and Technology, Yangzhou University)
  • 투고 : 2020.06.22
  • 심사 : 2020.09.20
  • 발행 : 2021.03.01

초록

Objective: The present study was conducted to investigate the effects of lycopene on growth performance, abdominal fat deposition, serum lipids levels, activities of hepatic lipid metabolism related enzymes and genes expression in broiler chickens. Methods: A total of 256 healthy one-day-old male Arbor Acres broiler chicks were randomly divided into four groups with eight replicates of eight birds each. Birds were fed basal diet supplemented with 0 (control), 100, 200, and 400 mg/kg lycopene, respectively. Results: Dietary 100 mg/kg lycopene increased the body weight at 21 day of age compared to the control group (p<0.05). Compared to the basal diet, broilers fed diet with 100 mg/kg lycopene had decreased abdominal fat weight, and broilers fed diet with 100 and 200 mg/kg lycopene had decreased abdominal fat percentage (p<0.05). Compared to control, diets with 100, 200, and 400 mg/kg lycopene reduced the levels of total triglyceride and total cholesterol in serum, and diets with 100 and 200 mg/kg lycopene reduced the level of serum low density lipoprotein cholesterol (p<0.05). The activity of fatty acid synthase (FAS) in 400 mg/kg lycopene treated broilers and the activity of acetyl-CoA carboxylase (ACC) in 100, 200, and 400 mg/kg lycopene treated broilers were lower than those fed basal diet (p<0.05). Lycopene increased the mRNA abundance of adenosine monophosphate activated protein kinase α (AMPK-α), whereas decreased the mRNA abundance of sterol regulatory element-binding protein 1, FAS, and ACC compared to the control group (p<0.05). Conclusion: Dietary lycopene supplementation can alleviate abdominal fat deposition and decrease serum lipids levels, possibly through activating the AMPK signaling pathway, thereby regulating lipid metabolism such as lipogenesis. Therefore, lycopene or lycopene-rich plant materials might be added to poultry feed to regulate lipid metabolism.

키워드

참고문헌

  1. Li Q, Zhao XL, Gilbert ER, et al. Confined housing system increased abdominal and subcutaneous fat deposition and gene expressions of carbohydrate response element-binding protein and sterol regulatory element-binding protein 1 in chicken. Genet Mol Res 2015;14:1220-8. https://doi.org/10.4238/2015.february.6.24
  2. Huang J, Zhang Y, Zhou Y, et al. Green tea polyphenols alleviate obesity in broiler chickens through the regulation of lipid-metabolism-related genes and transcription factor expression. J Agric Food Chem 2013;61:8565-72. https://doi.org/10.1021/jf402004x
  3. Chen G, Gao Z, Chu W, Cao Z, Li C, Zhao H. Effects of chromium picolinate on fat deposition, activity and genetic expression of lipid metabolism-related enzymes in 21 day old ross broilers. Asian-Australas J Anim Sci 2018;31:56975. https://doi.org/10.5713/ajas.17.0289
  4. Mirzaie S, Zirak-Khattab F, Hosseini SA, Donyaei-Darian H. Effects of dietary Spirulina on antioxidant status, lipid profile, immune response and performance characteristics of broiler chickens reared under high ambient temperature. Asian-Australas J Anim Sci 2018;31:556-63. https://doi.org/10.5713/ajas.17.0483
  5. Windisch W, Schedle K, Plitzner C, Kroismayr A. Use of phytogenic products as feed additives for swine and poultry. J Anim Sci 2008;86(Suppl 14):E140-8. https://doi.org/10.2527/jas.2007-0459
  6. Nobre BP, Palavra AF, Pessoa FLP, Mendes RL. Supercritical CO2 extraction of trans-lycopene from Portuguese tomato industrial waste. Food Chem 2009;116:680-5. https://doi.org/10.1016/j.foodchem.2009.03.011
  7. Liang X, Ma C, Yan X, Liu X, Liu F. Advances in research on bioactivity, metabolism, stability and delivery systems of lycopene. Trends Food Sci Technol 2019;93:185-96. https://doi.org/10.1016/j.tifs.2019.08.019
  8. Choi SK, Seo JS. Effect of lycopene supplementation on glucose and lipid metabolism in high fat diet-induced obese Mongolian gerbils. FASEB J 2012;26(Suppl 1):lb301. https://doi.org/10.1096/fasebj.26.1_supplement.lb301
  9. Kim AY, Jeong YJ, Park YB, et al. Dose dependent effects of lycopene enriched tomato-wine on liver and adipose tissue in high-fat diet fed rats. Food Chem 2012;130:42-8. https://doi.org/10.1016/j.foodchem.2011.06.050
  10. Thies F, Mills LM, Moir S, Masson LF. Cardiovascular benefits of lycopene: fantasy or reality? Proc Nutr Soc 2017;76:1229. https://doi.org/10.1017/S0029665116000744
  11. Sevcikova S, Skrivan M, Dlouha G. The effect of lycopene supplementation on lipid profile and meat quality of broiler chickens. Czech J Anim Sci 2008;53:431-40. https://doi.org/10.17221/350-CJAS
  12. Hosseini-Vashan SJ, Golian A, Yaghobfar A. Growth, immune, antioxidant, and bone responses of heat stress-exposed broilers fed diets supplemented with tomato pomace. Int J Biometeorol 2016;60:1183-92. https://doi.org/10.1007/s00484-015-1112-9
  13. Sahin K, Onderci M, Sahin N, Gursu MF, Khachik F, Kucuk O. Effects of lycopene supplementation on antioxidant status, oxidative stress, performance and carcass characteristics in heat-stressed Japanese quail. J Therm Biol 2006;31:307-12. https://doi.org/10.1016/j.jtherbio.2005.12.006
  14. Sun B, Chen C, Wang W, et al. Effects of lycopene supplementation in both maternal and offspring diets on growth performance, antioxidant capacity and biochemical parameters in chicks. J Anim Physiol Anim Nutr 2015;99:42-9. https://doi.org/10.1111/jpn.12196
  15. Bainor A, Chang L, McQuade TJ, Webb B, Gestwicki JE. Bicinchoninic acid (BCA) assay in low volume. Anal Biochem 2011;410:310-2. https://doi.org/10.1016/j.ab.2010.11.015
  16. Schmittgen TD, Livak KJ. Analyzing real-time PCR data by the comparative CT method. Nat Protoc 2008;3:1101-8. https://doi.org/10.1038/nprot.2008.73
  17. Lira RC, Rabello CBV, Ludke MCMM, Ferreira PV, Lana GRQ, Lana SRV. Productive performance of broiler chickens fed tomato waste. Rev Bras Zootec 2010;39:1074-81. https://doi.org/10.1590/S1516-35982010000500018
  18. Aidoud A, Ammouche A, Garrido M, Rodriguez AB. Effect of lycopene-enriched olive and argan oils upon lipid serum parameters in wistar rats. J Sci Food Agric 2014;94:2943-50. https://doi.org/10.1002/jsfa.6638
  19. Fenni S, Hammou H, Astier J, et al. Lycopene and tomato powder supplementation similarly inhibit high-fat diet induced obesity, inflammatory response, and associated metabolic disorders. Mol Nutr Food Res 2017;61:1601083. https://doi.org/10.1002/mnfr.201601083
  20. Jiang H, Wang Z, Ma Y, Qu Y, Lu X, Luo H. Effects of dietary lycopene supplementation on plasma lipid profile, lipid peroxidation and antioxidant defense system in feedlot Bamei lamb. Asian-Australas J Anim Sci 2015;28:958-65. https://doi.org/10.5713/ajas.14.0887
  21. Periago MJ, Martin-Pozuelo G, Gonzalez-Barrio R, et al. Effect of tomato juice consumption on the plasmatic lipid profile, hepatic HMGCR activity, and fecal short chain fatty acid content of rats. Food Funct 2016;7:4460-7. https://doi.org/10.1039/C6FO00344C
  22. Palozza P, Catalano A, Simone RE, Mele MC, Cittadini A. Effect of lycopene and tomato products on cholesterol metabolism. Ann Nutr Metab 2012;61:126-34. https://doi.org/10.1159/000342077
  23. Griffin HD, Guo K, Windsor D, Butterwith SC. Adipose tissue lipogenesis and fat deposition in leaner broiler chickens. J Nutr 1992;122:363-8. https://doi.org/10.1093/jn/122.2.363
  24. Cordero MD, Viollet B. AMP-activated protein kinase. Switzerland: Springer, Cham; 2016. https://doi.org/10.1007/978-3319-43589-3
  25. Hardie DG. AMPK: positive and negative regulation, and its role in whole-body energy homeostasis. Curr Opin Cell Biol 2015;33:1-7. https://doi.org/10.1016/j.ceb.2014.09.004
  26. Li Y, Xu S, Mihaylova MM, et al. AMPK phosphorylates and inhibits SREBP activity to attenuate hepatic steatosis and atherosclerosis in diet-induced insulin-resistant mice. Cell Metab 2011;13:376-88. https://doi.org/10.1016/j.cmet.2011.03.009
  27. Khesht FA, Hassanabadi A. Effects of sterol regulatory element-binding protein (srebp) in chickens. Lipids Health Dis 2012; 11:20. https://doi.org/10.1186/1476-511X-11-20
  28. Eberle D, Hegarty B, Bossard P, Ferre P, Foufelle F. SREBP transcription factors: master regulators of lipid homeostasis. Biochimie 2004;86:839-48. https://doi.org/10.1016/j.biochi.2004.09.018
  29. Angin Y, Beauloye C, Horman S, Bertrand L. Regulation of carbohydrate metabolism, lipid metabolism, and protein metabolism by AMPK. In: Cordero MD, Viollet B, editors. AMP-activated protein kinase. Switzerland: Springer, Cham; 2016. pp. 23-43. https://doi.org/10.1007/978-3-319-435893_2
  30. Liu M, Liu H, Xie J, et al. Anti-obesity effects of zeaxanthin on 3T3-L1 preadipocyte and high fat induced obese mice. Food Funct 2017;8:3327-38. https://doi.org/10.1039/C7FO00486A

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