Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Donggwaja Suppresses Inflammatory Reaction Via Tumor Necrosis Factor α-induced Protein3 and NF-κB

Tumor necrosis factor α - induced protein3의 발현과 NF-κB 활성 억제를 통한 동과자의 염증반응 억제 효과

  • Kim, Kyun Ha (Department of Internal Medicine, Pusan National University Korean Medicine Hospital) ;
  • Choi, Jun-Yong (Department of Internal Medicine, Pusan National University Korean Medicine Hospital) ;
  • Joo, Myungsoo (School of Korean Medicine, Pusan National University)
  • 김균하 (부산대학교 한방병원 한방내과) ;
  • 최준용 (부산대학교 한방병원 한방내과) ;
  • 주명수 (부산대학교 한의학전문대학원)
  • Received : 2021.01.04
  • Accepted : 2021.02.25
  • Published : 2021.02.25
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Abstract

Donggwaja (Benincasae Semen), the seed of Benincasa hispida (Thunb.) Cogn., has been used in Korean traditional medicine to control the body heat and water retention caused by various diseases. Both the symptoms targeted by the herbal medicine in clinic and studies with disease mouse models support the potential anti-inflammatory effect of Donggwaja. However, it is less understood how Donggwaja exerts its possible anti-inflammatory effect. Here, we present evidence that Donggwaja suppresses macrophage inflammatory reactions via expressing tumor necrosis factor a-induced protein 3 (TNFAIP3 or A20) and suppressing NF-kB activity. The ethanol extract of Donggwaja (EED) showed no toxicity when added to RAW 264.7 cells less than 100mg/ml. When treating the cells for 16 h, EED significantly suppressed the nuclear localization of NF-kB, suggesting that EED suppresses NF-kB activity. Concordantly, a semi-quantitative RT-PCR analysis showed that EED decreased the expression of prototypic pro-inflammatory cytokines, such as tumor necrosis factor (TNF)-a, IL(interleukin)-6, and IL-1b. EED induced in RAW 264.7 cells the expression of A20, a ubiquitin modulator that suppresses inflammatory signaling cascades initiated from TLR4 and TNF and IL-1 receptors, while not affecting the induction of Nrf2, an anti-inflammatory factor that could suppress the effect of NF-kB. These results suggest that EED exerts its suppressive effect on inflammation, at least in part, by expressing anti-inflammatory factor A20 and suppressing pro-inflammatory factor NF-kB activity.

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