The Korean Journal of Physiology and Pharmacology

The Korean Society of Pharmacology (대한약리학회)
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Sepsis induces variation of intestinal barrier function in different phase through nuclear factor kappa B signaling

  • Cao, Ying-Ya (Department of Intensive Care Unit, The First Affiliated Hospital of Wannan Medical College) ;
  • Wang, Zhong-Han (Department of Intensive Care Unit, The First Affiliated Hospital of Wannan Medical College) ;
  • Xu, Qian-Cheng (Department of Intensive Care Unit, The First Affiliated Hospital of Wannan Medical College) ;
  • Chen, Qun (Department of Intensive Care Unit, The First Affiliated Hospital of Wannan Medical College) ;
  • Wang, Zhen (Department of Intensive Care Unit, The First Affiliated Hospital of Wannan Medical College) ;
  • Lu, Wei-Hua (Department of Intensive Care Unit, The First Affiliated Hospital of Wannan Medical College)
  • Received : 2021.03.29
  • Accepted : 2021.06.02
  • Published : 2021.07.01
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Abstract

The intestinal barrier function disrupted in sepsis, while little is known about the variation in different phases of sepsis. In this study, mouse models of sepsis were established by caecal ligation and puncture (CLP). The H&E staining of sections and serum diamine oxidase concentration were evaluated at different timepoint after CLP. TUNEL assay and EdU staining were performed to evaluate the apoptosis and proliferation of intestinal epithelium. Relative protein expression was assessed by Western blotting and serum concentrations of pro-inflammatory cytokines was measured by ELISA. The disruption of intestinal barrier worsened in the first 24 h after the onset of sepsis and gradually recovered over the next 24 h. The percentage of apoptotic cell increased in the first 24 h and dropped at 48 h, accompanied with the proliferative rate of intestinal epithelium inhibited in the first 6 h and regained in the later period. Furthermore, the activity of nuclear factor kappa B (NF-κB) presented similar trend with the intestinal barrier function, shared positive correction with apoptosis of intestinal epithelium. These findings reveal the conversion process of intestinal barrier function in sepsis and this process is closely correlated with the activity of NF-κB signaling.

Keywords

Acknowledgement

This study was supported by the National Natural Science Foundation of China (82002092), the Anhui Province Natural Science Foundation for Youth (1908085QH360) and funding for the 'Peak' Training Programme for Scientific Research of Yijishan Hospital, Wannan Medical College (GF2019J03 and GF2019G08).

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