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DOI QR Code

Relationships among bedding materials, bedding bacterial composition and lameness in dairy cows

  • Li, Han (Department of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agricultural University) ;
  • Wang, Xiangming (Department of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agricultural University) ;
  • Wu, Yan (Department of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agricultural University) ;
  • Zhang, Dingran (Department of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agricultural University) ;
  • Xu, Hongyang (Department of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agricultural University) ;
  • Xu, Hongrun (Department of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agricultural University) ;
  • Xing, Xiaoguang (Department of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agricultural University) ;
  • Qi, Zhili (Department of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agricultural University)
  • 투고 : 2020.08.11
  • 심사 : 2020.10.27
  • 발행 : 2021.09.01

초록

Objective: Bedding materials directly contact hooves of dairy cows and they may serve as environmental sources of lameness-associated pathogen. However, the specific composition of bacteria hidden in bedding materials is still not clear. The aim of this study was to determine the effect bedding material and its bacterial composition has on lameness of Holstein heifers. Methods: Forty-eight Holstein heifers with similar body weights were randomly assigned into three groups including sand bedding (SB), concrete floor (CF), and compost bedding (CB). Hock injuries severity and gait performance of dairy cows were scored individually once a week. Blood samples were collected at the end of the experiment and bedding material samples were collected once a week for Illumina sequencing. Results: The CF increased visible hock injuries severity and serum biomarkers of joint damage in comparison to SB and CB groups. Besides, Illumina sequencing and analysis showed that the bacterial community of CB samples had higher similarity to that of SB samples than CF samples. Bacteria in three bedding materials were dominated by gastrointestinal bacteria and organic matter-degrading bacteria, such as Actinobacteria, Firmicutes, and norank JG30-KF-cM45. Lameness-associated Spirochaetaceae and Treponeme were only detected in SB and CB samples with a very low relative abundance (0% to 0.08%). Conclusion: The bacterial communities differed among bedding materials. However, the treponemes pathogens involved in the pathogenesis of lameness may not be a part of microbiota in bedding materials of dairy cows.

키워드

과제정보

This work was supported by the National Key Research and Development Program of China (grant number 2018YFD0501605); the Fundamental Research Funds for the Central Universities (grant number 2662018Y079); and the Hubei Key Laboratory of Animal Nutrition (grant number 2004DA125184F1721).

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피인용 문헌

  1. Assessing the microbiota of recycled bedding sand on a Wisconsin dairy farm vol.12, pp.1, 2021, https://doi.org/10.1186/s40104-021-00635-6