The Korean Journal of Physiology and Pharmacology

  • 제25권5호
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  • Pages.439-448
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  • 2021
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  • 1226-4512(pISSN)
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  • 2093-3827(eISSN)
대한약리학회 (The Korean Society of Pharmacology)
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Anti-inflammatory effects of DA-9601, an extract of Artemisia asiatica, on aceclofenac-induced acute enteritis

  • Kim, Ju Hwan (Department of Pharmacology, College of Medicine, Dankook University) ;
  • Shin, Chang Yell (Research Institute of Dong-A ST Co., Ltd.) ;
  • Jang, Sun Woo (Research Institute of Dong-A ST Co., Ltd.) ;
  • Kim, Dong-Seok (Department of Biochemistry, College of Medicine, Chung-Ang University) ;
  • Lee, Wonae (Department of Pathology, College of Medicine, Dankook University) ;
  • Kim, Hyung-Gun (Department of Pharmacology, College of Medicine, Dankook University) ;
  • Kim, Hak Rim (Department of Pharmacology, College of Medicine, Dankook University)
  • 투고 : 2021.02.26
  • 심사 : 2021.05.06
  • 발행 : 2021.09.01
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초록

DA-9601 is an extract obtained from Artemisia asiatica, which has been reported to have anti-inflammatory effects on gastrointestinal lesions; however, its possible anti-inflammatory effects on the small intestine have not been studied yet. Therefore, in this study, we investigated the protective effects of DA-9601 against the ACF-induced small intestinal inflammation. Inflammation of the small intestine was confirmed by histological studies and the changes in the CD4+ T cell fraction induced by the inflammation-related cytokines, and the inflammatory reactions were analyzed. Multifocal discrete small necrotic ulcers with intervening normal mucosa were frequently observed after treatment with ACF. The expression of IL-6, IL-17, and TNF-α genes was increased in the ACF group; however, it was found to have been significantly decreased in the DA-9601 treated group. In addition, DA-9601 significantly decreased the levels of proinflammatory mediators such as IL-1β, GM-CSF, IFN-γ, and TNF-α; the anti-inflammatory cytokine IL-10, on the other hand, was observed to have increased. It is known that inflammatory mediators related to T cell imbalance and dysfunction continuously activate the inflammatory response, causing chronic tissue damage. The fractions of IFN-γ+ Th1 cells, IL-4+ Th2 cells, IL-9+ Th9 cells, IL-17+ Th17 cells, and Foxp3+ Treg cells were significantly decreased upon DA-9601 treatment. These data suggest that the inflammatory response induced by ACF is reduced by DA-9601 via lowering of the expression of genes encoding the inflammatory cytokines and the concentration of inflammatory mediators. Furthermore, DA-9601 inhibited the acute inflammatory response mediated by T cells, resulting in an improvement in ACF-induced enteritis.

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