Pediatric Gastroenterology, Hepatology & Nutrition

  • 제24권5호
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  • Pages.443-454
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  • 2021
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  • 2234-8646(pISSN)
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  • 2234-8840(eISSN)
대한소아소화기영양학회 (The Korean Society of Pediatric Gastroenterology, Hepatology and Nutrition)
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Investigation of Thiol/Disulfide Balance in Obese Rats with Non-Alcoholic Fatty Liver Disease

  • Tursun, Serkan (Department of Pediatrics, Faculty of Medicine, Kirikkale University) ;
  • Gulerman, Hacer Fulya (Department of Pediatric Gastroenterology, Faculty of Medicine, Kirikkale University) ;
  • Gazyagci, Serkal (Department of Internal Medicine, Faculty of Veterinary Medicine, Kirikkale University) ;
  • Sahin, Yasar (Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Kirikkale University) ;
  • Erel, Ozcan (Department of Biochemistry, Faculty of Medicine, Yildirim Beyazit University) ;
  • Neselioglu, Salim (Department of Biochemistry, Faculty of Medicine, Yildirim Beyazit University)
  • 투고 : 2020.12.01
  • 심사 : 2021.06.03
  • 발행 : 2021.09.15
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초록

Purpose: Due to the increasing prevalence of obesity worldwide, non-alcoholic fatty liver disease (NAFLD) has reached epidemic dimensions over time. NAFLD is the most common cause of childhood chronic liver disease. There is a relationship between NAFLD and oxidative stress. This study aims to investigate the changes in thiol/disulfide homeostasis parameters to determine the oxidant/antioxidant balance in obese rats with diet-induced NAFLD and healthy rats. Methods: Twelve Wistar albino rats were used in this study. Experimentally produced NAFLD obese rats (n=6) and healthy rats were compared. Experimental NAFLD model was created with a special fatty liver diet (Altromin® C1063, Fatty Liver Diet, Exclusivet, Lage, Germany). The biochemical and histopathological features of the groups, as well as serum thiol/disulfide homeostasis parameters, were analyzed and compared. Results: In the experimentally induced NAFLD rat model, they gained more weight than the control group. Steatosis (at least grade 2) occurred in all rats fed with special fatty liver diet for 12 weeks. Histopathologically, no high-grade inflammation was observed in rats with experimental NAFLD after feeding a diet for 12 weeks. Results revealed that aspartate transaminase and alanine transaminase levels were high, albumin levels were low, oxidant stress parameters increased, and antioxidant thiol groups decreased. Conclusion: Experimental NAFLD is characterized by increased oxidant stress accompanying fatty tissue in the liver. Analysis of thiol/disulfide homeostasis parameters in NAFLD can be used in further studies to develop effective treatment options.

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과제정보

All authors would like to thank Irfan Karahan for his contribution to the redaction of this article.

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