Animal Bioscience

  • 제34권11호
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  • Pages.1766-1775
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  • 2021
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  • 2765-0189(pISSN)
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  • 2765-0235(eISSN)
아세아태평양축산학회 (Asian Australasian Association of Animal Production Societies)
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The effect of light on follicular development in laying hens

  • Cheng, Shi Bin (College of Animal Science, Yangtze University) ;
  • Li, Xian Qiang (College of Animal Science, Yangtze University) ;
  • Wang, Jia Xiang (College of Animal Science, Yangtze University) ;
  • Wu, Yan (Institute of Animal Husbandry and Veterinary, Hubei Academy of Agricultural Science) ;
  • Li, Peng (College of Animal Science, Yangtze University) ;
  • Pi, Jin Song (Institute of Animal Husbandry and Veterinary, Hubei Academy of Agricultural Science)
  • 투고 : 2020.11.18
  • 심사 : 2021.04.06
  • 발행 : 2021.11.01
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초록

Objective: The oxidative stress status and changes of chicken ovary tissue after shading were studied, to determine the mechanism of the effect of shading on follicular development. Methods: Twenty healthy laying hens (40 weeks old) with uniform body weight and the same laying rate were randomly divided into two groups (the shading group and normal light group). In the shading group, the cage was covered to reduce the light intensity inside the cage to 0 without affecting ventilation or food intake. The normal lighting group received no additional treatment. After 7 days of shading, oxidative stress related indicators and gene expression were detected. Results: Analysis of paraffin and ultrathin sections showed that apoptosis of ovarian granulosa cells (GCs) increased significantly after light shading. Enzyme linked immunosorbent assay results revealed that the levels of total antioxidant capacity, malondialdehyde, superoxide dismutase (SOD), glutathione, catalase (CAT), and other substances in the sera, livers, ovaries, and follicular GCs of laying hens increased significantly after shading for 7 days; and reactive oxygen species (ROS) levels in the livers of laying hens also increased significantly. ROS in the serum, ovarian and GCs also increased. After shading for 7 days, the levels of 8-hydroxy-2 deoxyguanosine in the sera and ovarian tissues of laying hens increased significantly. Cell counting kit-8 detection showed that the proliferation activity of GCs in layer follicles decreased after shading for 7 days; the expression level of the anti-apoptotic gene B-cell lymphoma-2 in ovarian tissue and follicular GCs was significantly reduced, and the expression levels of pro-apoptotic caspase 3 (casp3), and SOD, glutathione peroxidase 2 (GPX2), and CAT were all significantly increased. Conclusion: Oxidative stress induced by shading light has a serious inhibitory effect on follicular development during reproduction in laying hens.

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과제정보

We would like to thank the Science and Technology Department of Hubei Province, and the Hubei Academy of Agricultural Sciences. This work was supported by the technology innovation special major project of Hubei Province (grant number 2019ABA084) and the open project of Animal Embryo and Molecular Breeding of Hubei Key Laboratory (grant number KLAEMB-2018-01).

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