Animal Bioscience

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Effects of starvation-induced negative energy balance on endoplasmic reticulum stress in the liver of cows

  • Islam, Md Aminul (Department of Biomedical Engineering, Graduate School of Medicine, Science and Technology, Shinshu University) ;
  • Adachi, Shuya (Department of Biomedical Engineering, Graduate School of Science and Technology, Shinshu University) ;
  • Shiiba, Yuichiroh (Faculty of Agriculture, Shinshu University) ;
  • Takeda, Ken-ichi (Faculty of Agriculture, Shinshu University) ;
  • Haga, Satoshi (Grazing Animal Unit, Division of Grassland Farming, Institute of Livestock and Grassland Science, NARO) ;
  • Yonekura, Shinichi (Department of Biomedical Engineering, Graduate School of Medicine, Science and Technology, Shinshu University)
  • Received : 2021.03.24
  • Accepted : 2021.05.27
  • Published : 2022.01.01
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Abstract

Objective: Endoplasmic reticulum (ER) stress engages the unfolded protein response (UPR) that serves as an important mechanism for modulating hepatic fatty acid oxidation and lipogenesis. Chronic fasting in mice induced the UPR activation to regulate lipid metabolism. However, there is no direct evidence of whether negative energy balance (NEB) induces ER stress in the liver of cows. This study aimed to elucidate the relationship between the NEB attributed to feed deprivation and ER stress in bovine hepatocytes. Methods: Blood samples and liver biopsy tissues were collected from 6 non-lactating cows before and after their starvation for 48 h. The blood non-esterified fatty acids (NEFA), β-hydroxybutyric acid (BHBA) and glucose level were analyzed. Real-time quantitative polymerase chain reaction and Western blotting were used to explore the regulation of genes associated with UPR and lipid metabolism. Results: The starvation increased the plasma BHBA and NEFA levels and decreased the glucose level. Additionally, the starvation caused significant increases in the mRNA expression level of spliced X-box binding protein 1 (XBP1s) and the protein level of phosphorylated inositol-requiring kinase 1 alpha (p-IRE1α; an upstream protein of XBP1) in the liver. The mRNA expression levels of peroxisome proliferator-activated receptor alpha and its target fatty acid oxidation- and ketogenesis-related genes were significantly upregulated by the starvation-mediated NEB. Furthermore, we found that the mRNA expression levels of lipogenic genes were not significantly changed after starvation. Conclusion: These findings suggest that in the initial stage of NEB in dairy cows, the liver coordinates an adaptive response by activating the IRE1 arm of the UPR to enhance ketogenesis, thereby avoiding a fatty liver status.

Keywords

Acknowledgement

This study was supported by JSPS KAKENHI Grant Numbers JP20H03126 to SY.

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