Journal of Forest and Environmental Science

  • 제38권3호
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  • Pages.152-158
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  • 2022
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  • 2288-9744(pISSN)
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  • 2288-9752(eISSN)
강원대학교 산림과학연구소 (Institute of Forest Science, kangwon National University)
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Comparison of Cryoprotectants and Cryopreservation Protocols for Eleutherococcus senticosus via Somatic Embryogenesis

  • Ahn, Chang Ho (Division of Garden Material Research, Korea Institute of Arboretum Management) ;
  • Shin, Jung Won (Division of Garden Material Research, Korea Institute of Arboretum Management) ;
  • Lee, Ha Na (Division of Garden Material Research, Korea Institute of Arboretum Management) ;
  • Yoon, Hyun Won (Division of Garden Material Research, Korea Institute of Arboretum Management) ;
  • Seo, Jeong Min (Division of Garden Material Research, Korea Institute of Arboretum Management) ;
  • Kim, Yeoung Ryul (Division of Garden Material Research, Korea Institute of Arboretum Management) ;
  • Baek, Saeng Geul (Division of Garden Material Research, Korea Institute of Arboretum Management) ;
  • Nam, Jae Ik (Division of Garden Material Research, Korea Institute of Arboretum Management) ;
  • Choi, Yong Eui (Division of Forest Sciences, College of Forest and Environmental Sciences, Kangwon National University)
  • 투고 : 2022.06.24
  • 심사 : 2022.08.20
  • 발행 : 2022.09.30
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초록

A cryopreservation is an essential tool for preservation of germplasm. In this study, the possibility for cryopreservation of embryogenic cells of Siberian ginseng (Eleutherococcus senticosus) in liquid nitrogen (-196℃) was evaluated. The effects of glycerol and dimethyl sulfoxide (DMSO) at different concentrations (5%, 10% and 20%) as cryoprotectants on regrowth of cryopreserved E. senticosus embryogenic cells were tested. There was significant effect of cryoprotectants on regrowth of embryogenic cells (p=0.0019). The highest and lowest fresh mass gain were achieved when embryogenic cells were frozen with 10% DMSO and 5% glycerol (138.2±5.9 and 61.3±14.6, respectively). The effect of the cryoprotectants on the frequency embryo germination was tested. There was no significant difference between glycerol and DMSO (p=0.846). Three different concentrations of cryoprotectants did not significantly affect the frequency embryo germination (p=0.534). Finally, the genetic fidelity of the plantlets regenerated from non-cryopreserved and cryopreserved embryogenic cells was tested by random amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) analysis. RAPD and ISSR analysises showed that there was no genetic variation among regenerants.

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참고문헌

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