The Korean Journal of Physiology and Pharmacology

The Korean Society of Pharmacology (대한약리학회)
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Neogambogic acid relieves myocardial injury induced by sepsis via p38 MAPK/NF-κB pathway

  • Fu, Wei (Department of Emergency, Second Affiliated Hospital of Nanchang University) ;
  • Fang, Xiaowei (Department of Emergency, Second Affiliated Hospital of Nanchang University) ;
  • Wu, Lidong (Department of Emergency, Second Affiliated Hospital of Nanchang University) ;
  • Hu, Weijuan (Department of Emergency, Second Affiliated Hospital of Nanchang University) ;
  • Yang, Tao (Department of Emergency, Second Affiliated Hospital of Nanchang University)
  • Received : 2022.07.11
  • Accepted : 2022.08.16
  • Published : 2022.11.01
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Abstract

Sepsis-associated myocardial injury, an invertible myocardial depression, is a common complication of sepsis. Neogambogic acid is an active compound in garcinia and exerts anthelmintic, anti-inflammatory, and detoxification properties. The role of neogambogic acid in sepsis-associated myocardial injury was assessed. Firstly, mice were pretreated with neogambogic acid and then subjected to lipopolysaccharide treatment to induce sepsis. Results showed that lipopolysaccharide treatment induced up-regulation of biomarkers involved in cardiac injury, including lactate dehydrogenase (LDH), creatine kinase-MB (CK-MB), and troponin I (cTnI). However, pretreatment with neogambogic acid reduced levels of LDH, CK-MB, and cTnI, and ameliorated histopathological changes in the heart tissues of septic mice. Secondly, neogambogic acid also improved cardiac function in septic mice through reduction in left ventricular end-diastolic pressure, and enhancement of ejection fraction, fractional shortening, and left ventricular systolic mean pressure. Moreover, neogambogic acid suppressed cardiac apoptosis and inflammation in septic mice and reduced cardiac fibrosis. Lastly, protein expression of p-p38, p-JNK, and p-NF-κB in septic mice was decreased by neogambogic acid. In conclusion, neogambogic acid exerted anti-apoptotic, anti-fibrotic, and anti-inflammatory effects in septic mice through the inactivation of MAPK/NF-κB pathway.

Keywords

Acknowledgement

This work was supported by the Science and Technology Plan of Jiangxi Provincial Health Commission (Grant No. 20204331).

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