Animal Bioscience

  • 제35권11호
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  • Pages.1666-1674
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  • 2022
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  • 2765-0189(pISSN)
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  • 2765-0235(eISSN)
아세아태평양축산학회 (Asian Australasian Association of Animal Production Societies)
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Letrozole, an aromatase inhibitor, improves seminal parameters and hormonal profile in aged endangered Markhoz bucks

  • Rezaei, Ako (Department of Animal Science, Faculty of Agriculture, University of Kurdistan) ;
  • Vaziry, Asaad (Department of Animal Science, Faculty of Agriculture, University of Kurdistan) ;
  • Farshad, Abbas (Department of Animal Science, Faculty of Agriculture, University of Kurdistan)
  • 투고 : 2021.06.12
  • 심사 : 2021.08.15
  • 발행 : 2022.11.01
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초록

Objective: Letrozole, a potent aromatase inhibitor, is known to have the potential to modify male reproductive function by altering sex hormone levels. This study aimed to evaluate the semen and testicular characteristics and hormonal profile of aged Mrakhoz bucks (Capra hircus) treated with letrozole. Methods: Twelve Markhoz male goats, aged between 4.5 to 5.5 years with an average body weight (BW) of 61.05±4.97 kg were used for the study. Animals were randomly divided into two equal groups and subcutaneously received either 0.25 mg/kg BW of letrozole or a control every week for 2 months. The semen collections were performed every 10 days, and blood samples and testicular biometric records were collected at 20 days intervals. Results: Letrozole causes increased testosterone and follicle-stimulating hormone levels, testosterone to estradiol ratio, semen index and reaction time during the period from 20th to 60th days (p<0.05). Furthermore, letrozole-treated bucks had higher semen volume, sperm concentration, and total sperm per ejaculate from 30th to 60th days (p<0.05). However, no differences occurred between the groups in scrotal circumference, relative testicular volume, semen pH, abnormality, acrosome integrity, and membrane integrity of sperm during the study (p>0.05). The serum luteinizing hormone levels, sperm viability, motility, and progressive motility increased, and estradiol levels decreased after 40th to 60th days of letrozole treatment (p<0.05). Conclusion: Letrozole application to aged Markhoz bucks provokes reproductive hormonal axis which, in turn, induces enhancement of semen production and quality.

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과제정보

The authors thank the University of Kurdistan for supporting this work. We also want to express our gratitude to Kurdistan Agricultural and Natural Resources Research and Education Center for their encouragement and assistance during this research. The authors also gratefully thank the Director of Sanandaj Markhoz Goat Husbandry Research Station; Mr. S. Rashidi for providing the necessary to conduct farm operations, and Mr. E. Golmoradi for assistance in experimental work.

참고문헌

  1. Bahmani HR, Tahmoorespur M, Aslaminejad AA, Vatankhah M, Rashidi A. Simulating past dynamics and assessing current status of Markhoz goat population on its habitat. Iran J Appl Anim Sci 2015;5:347-54.
  2. Goyal HO, Menon MA. Clinical reproductive anatomy and physiology of the Buck. In: Current therapy in large animal theriogenology. Second edition, Elsevier; 2007, p. 511-4. https://doi.org/10.1016/B978-072169323-1.50067-2
  3. Talebi J, Souri M, Moghaddam A, Karimi I, Mirmahmoodi M. Characteristics and seasonal variation in the semen of Markhoz bucks in western Iran. Small Rumin Res 2009;85: 18-22. https://doi.org/10.1016/j.smallrumres.2009.06.017
  4. Midzak AS, Chen H, Papadopoulos V, Zirkin BR. Leydig cell aging and the mechanisms of reduced testosterone synthesis. Mol Cell Endocrinol 2009;299:23-31. https://doi.org/10.1016/j.mce.2008.07.016
  5. Ehmcke J, Schlatt S. Animal models for fertility preservation in the male. Reproduction 2008;136:717-23. https://doi.org/10.1530/REP-08-0093
  6. Schlegel PN. Aromatase inhibitors for male infertility. Fertil Steril 2012;98:1359-62. https://doi.org/10.1016/j.fertnstert.2012.10.023
  7. Dias JP, Melvin D, Shardell M, et al. Effects of transdermal testosterone gel or an aromatase inhibitor on prostate volume in older men. J Clin Endocrinol Metab 2016;101:1865-71. https://doi.org/10.1210/jc.2016-1111
  8. T'Sjoen GG, Giagulli VA, Delva H, Crabbe P, De Bacquer D, Kaufman J-M. Comparative assessment in young and elderly men of the gonadotropin response to aromatase inhibition. J Clin Endocrinol Metab 2005;90:5717-22. https://doi.org/10.1210/jc.2005-0982
  9. Loves S, Ruinemans-Koerts J, de Boer H. Letrozole once a week normalizes serum testosterone in obesity-related male hypogonadism. Eur J Endocrinol 2008;158:741-7. https://doi.org/10.1530/EJE-07-0663
  10. Cavallini G, Beretta G, Biagiotti G. Preliminary study of letrozole use for improving spermatogenesis in non-obstructive azoospermia patients with normal serum FSH. Asian J Androl 2011;13:895-7. https://doi.org/10.1038/aja.2011.44
  11. Kooshesh L, Bahmanpour S, Zeighami S, Nasr-Esfahani MH. Effect of Letrozole on sperm parameters, chromatin status and ROS level in idiopathic Oligo/Astheno/Teratozoospermia. Reprod Biol Endocrinol 2020;18:47. https://doi.org/10.1186/s12958-020-00591-2
  12. Peivandi S, Jafarpour H, Abbaspour M, Ebadi A. Effect of letrozole on spermogram parameters and hormonal profile in infertile men: a clinical trial study. Endocr Regul 2019;53: 231-6. https://doi.org/10.2478/enr-2019-0023
  13. Stein TA, Ball BA, Conley AJ, Bhatnagar AS, Roser JF. The effects of an aromatase inhibitor (Letrozole) on hormone and sperm production in the stallion. Theriogenology 2002; 58:381-3. https://doi.org/10.1016/S0093-691X(02)00873-7
  14. At-Taras EE. Reducing estrogen synthesis in developing boars increases testis size and total sperm production. J Androl 2006;27:552-9. https://doi.org/10.2164/jandrol.05195
  15. Berger T, Conley AJ, Van Klompenberg M, Roser JF, Hovey RC. Increased testicular Sertoli cell population induced by an estrogen receptor antagonist. Mol Cell Endocrinol 2013; 366:53-8. https://doi.org/10.1016/j.mce.2012.11.011
  16. Bazyar M, Sharafi M, Shahverdi A. Changes in seminal parameters and hormonal profile with use of aromatase inhibitor in management of aging broiler breeder roosters. Poult Sci 2019;98:6100-7. https://doi.org/10.3382/ps/pez325
  17. Adeldust H, Farzinpour A, Farshad A, Rostamzadeh J, LopezBejar M. Increased sperm cell production in ageing roosters by an oral treatment with an aromatase inhibitor and a natural herbal extract designed for improving fertility. Reprod Domest Anim 2017;52:58-60. https://doi.org/10.1111/rda.13058
  18. Ali EA, Zhandi M, Towhidi A, et al. Letrozole, an aromatase inhibitor, reduces post-peak age-related regression of rooster reproductive performance. Anim Reprod Sci 2017;183:110-7. https://doi.org/10.1016/j.anireprosci.2017.05.010
  19. Adeldust H, Farzinpour A, Farshad A, Rostamzadeh J, Lopez Bejar M. Effect of orally administrated letrozole on reproduction performance and gene expression of FOXJ1, LPR2 and PVRL3 in reproductive tract in aged roosters. Theriogenology 2021;161:131-9. https://doi.org/10.1016/j.theriogenology.2020.11.020
  20. Rezaei A, Vaziry A, Farshad A, Farzinpour A, Rostamzadeh J. Effects of letrozole administration on growth and reproductive performance in Markhoz goat bucklings. Theriogenology 2020;147:183-91. https://doi.org/10.1016/j.theriogenology.2019.11.017
  21. Ramadan TA, Taha TA, Samak MA, Hassan A. Effectiveness of exposure to longday followed by melatonin treatment on semen characteristics of Damascus male goats during breeding and non-breeding seasons. Theriogenology 2009;71:458-68. https://doi.org/10.1016/j.theriogenology.2008.07.029
  22. Ogundele FA, Okubanjo OO, Ajanusi OJ, Fadason ST. Semen characteristics and reaction time of Yankasa rams experimentally infected with Trypanosoma evansi infection. Theriogenology 2016;86:667-73. https://doi.org/10.1016/j.theriogenology.2016.02.018
  23. Farshad A, Yousefi A, Moghaddam A, Khalili B. Seasonal changes in serum testosterone, LDH concentration and semen characteristics in Markhoz goats. Asian-Australas J Anim Sci 2012;25:189-93. https://doi.org/10.5713/ajas.2011.11179
  24. Turner K, Morley M, Atanassova N, Swanston I, Sharpe R. Effect of chronic administration of an aromatase inhibitor to adult male rats on pituitary and testicular function and fertility. J Endocrinol 2000;164:225-38. https://doi.org/10.1677/joe.0.1640225
  25. Berger T. Testicular estradiol and the pattern of Sertoli cell proliferation in prepuberal bulls. Theriogenology 2019;136: 60-5. https://doi.org/10.1016/j.theriogenology.2019.06.031
  26. Banerjee A, Anuradha, Mukherjee K, Krishna A. Testicular glucose and its transporter GLUT 8 as a marker of age-dependent variation and its role in steroidogenesis in mice. J Exp Zool Part A Ecol Genet Physiol 2014;321:490-502. https://doi.org/10.1002/jez.1881
  27. Muratori M, Baldi E. Effects of FSH on sperm DNA fragmentation: review of clinical studies and possible mechanisms of action. Front Endocrinol 2018;9:734. https://doi.org/10.3389/fendo.2018.00734
  28. Farzinpour A, Vaziry A, Mohammadi S. Immunohistochemical localization of aromatase in goat testicular tissue and accessory glands. Vet Res Biol Prod 2015:83-9. https://doi.org/10.22092/vj.2015.103034
  29. Angel-Garcia O, Meza-Herrera CA, Guillen-Munoz JM, et al. Seminal characteristics, libido and serum testosterone concentrations in mixed-breed goat bucks receiving testosterone during the non-breeding period. J Appl Anim Res 2015;43:457-61. https://doi.org/10.1080/09712119.2014.980420
  30. Fritz WF, Sena LS, Becker SE, Katz LS. Differential effects of androgens, estrogens and socio-sexual context on sexual behaviors in the castrated male goat. Horm Behav 2019; 109:10-7. https://doi.org/10.1016/j.yhbeh.2019.01.008