Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Antidiabetic Effect of Aurantii Fructus Immaturus in Streptozotocin-induced Diabetes Model of Mice

Streptozotocin 유도 당뇨병 생쥐 모델에서 지각 추출물의 항당뇨 효과

  • Kyung-Jae Yi (Department of Third Medicine of Korean Medicine, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Ji-Sung Im (Department of Korean Medicine Rehabilitation, College of Korean Medicine Wonkwang University) ;
  • Ji-Eun Kim (Department of Korean Medicine Rehabilitation, College of Korean Medicine Wonkwang University) ;
  • Su-Kyung Lee (Department of Korean Medicine Rehabilitation, College of Korean Medicine Wonkwang University) ;
  • Hyun-Joo Kim (Department of Third Medicine of Korean Medicine, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Yung-Sun Song (Department of Third Medicine of Korean Medicine, Professional Graduate School of Oriental Medicine, Wonkwang University)
  • 이경재 (원광대학교 한의학전문대학원 제3의학과) ;
  • 임지성 (원광대학교 한의과대학 한방재활의학교실) ;
  • 김지은 (원광대학교 한의과대학 한방재활의학교실) ;
  • 이수경 (원광대학교 한의과대학 한방재활의학교실) ;
  • 김현주 (원광대학교 한의학전문대학원 제3의학과) ;
  • 송용선 (원광대학교 한의학전문대학원 제3의학과)
  • Received : 2022.10.07
  • Accepted : 2022.12.26
  • Published : 2023.02.25
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Abstract

The aim of this study is to evaluate the antidiabetic effect of the water extract of Aurantii fructus immaturus (WAF), in diabetic models using enzyme, cells and mice, and to suggest a putative mechanism explaining its antidiabetic effect. In an enzyme model using the enzyme α-glucosidase, WAF had no significant effect on α-glucosidase, as compared with acarbose, an antidiabetic drug. Nonetheless, WAF was capable of reducing the blood glucose levels during oral sucrose tolerance test and oral glucose tolerance test, implying that there would be other antidiabetic pathways in no relation to inhibition of α-glucosidase. In cell models using RIN-m5f β-cells and L6 myotubes, WAF, at its non-cytotoxic doses, augmented the secretion of insulin in RIN-m5f β-cells stimulated with 5 mM glucose. In addition, it enhanced the cellular uptake of glucose in L6 myotubes stimulated with deprivation of glucose for 12 h. Therefore, it is most likely that WAF may exert its antidiabetic effects, at least in part, by enhancing insulin secretion and glucose uptake. Meanwhile, in diabetic mice induced with peritoneal injection of streptozotocin (STZ), WAF significantly improved fast blood glucose levels, glycosylated hemoglobin levels, body weight loose, blood pressure, and diabetic adverse effects on functions of the kidney and the liver. Taken together, the water extract of Aurantii fructus immaturus may ameliorate diabetes in mice injected with STZ, at least in part, by enhancing insulin secretion and glucose uptake.

Keywords

Acknowledgement

본 연구는 2021년도 원광대학교 교비 지원에 의하여 연구되었음.

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