CELLMED (셀메드)

Cellmed Orthocellular Medicine and Pharmaceutical Association (셀메드 세포교정의약학회)
권호 표지
DOI QR코드

DOI QR Code

Comparison of Anti-Inflammation Effects of Specimens Before and After the Oil Extraction of Raphanus sativus L. Seed in RAW 264.7 Macrophage Activated by LPS

  • Received : 2023.05.11
  • Accepted : 2023.05.19
  • Published : 2023.05.31
Download PDF
⟨ Previous Next ⟩

Abstract

Raphanus sativus L. has been reported to have anti-inflammatory and anti-tumor activity. However, the anti-inflammatory effect and mechanism of action of the Raphanus sativus L. seeds (RSS) with or without oil are still unknown. This study was undertaken to investigate the in-vitro anti-inflammatory effect with or without oil in the RSS on RAW 264.7 cells stimulated by lipopolysaccharide (LPS). Results showed the suppressed LPS-induced secretion of pro-inflammatory mediators such as nitric oxide (NO), inflammatory cytokine (IL-6, TNF-α). Additionally, a decrease in protein expression of iNOS was observed, but nuclear translocation of NF-κB p65 was not inhibited. To elucidate the underlying mechanism of the anti-inflammatory effect of RSS, the involvement of mitogen-activated protein kinase (MAPK) signaling pathways was examined. We also found that RSS blocked LPS-induced phosphorylation of c-Jun N-terminal kinase/stress-activated protein kinase (JNK) signaling but did not affect the phosphorylation of p38 MAPK and extracellular signal-regulated kinase (ERK) 1/2. These results suggest that RSS may have potential as an anti-inflammatory agent through the inhibition of LPS-induced inflammatory cytokine production via regulation of the JNK pathway.

Keywords

Acknowledgement

Thank you CellMed for helping us write our thesis.

References

  1. Chang, Y.-H. et al. Bioconverted Jeju Hallabong tangor (Citrus kiyomi× ponkan) peel extracts by cytolase enhance antioxidant and anti-inflammatory capacity in RAW 264.7 cells. Nutrition research and practice 10, 131-138 (2016).  https://doi.org/10.4162/nrp.2016.10.2.131
  2. Ciesielska, A., Matyjek, M. & Kwiatkowska, K. TLR4 and CD14 trafficking and its influence on LPS-induced pro-inflammatory signaling. Cellular and molecular life sciences 78, 1233-1261 (2021).  https://doi.org/10.1007/s00018-020-03656-y
  3. CHEN, R. M. et al. Anti-inflammatory and antioxidative effects of propofol on lipopolysaccharide-activated macrophages. Annals of the New York Academy of Sciences 1042, 262-271 (2005).  https://doi.org/10.1196/annals.1338.030
  4. Liang, Y.-C. et al. Suppression of inducible cyclooxygenase and inducible nitric oxide synthase by apigenin and related flavonoids in mouse macrophages. Carcinogenesis 20, 1945-1952 (1999).  https://doi.org/10.1093/carcin/20.10.1945
  5. Hotamisligil, G. S. Inflammation, metaflammation and immunometabolic disorders. Nature 542, 177-185 (2017).  https://doi.org/10.1038/nature21363
  6. Cobb, M. H. & Goldsmith, E. J. How MAP Kinases Are Regulated (*). Journal of Biological Chemistry 270, 14843-14846 (1995).  https://doi.org/10.1074/jbc.270.25.14843
  7. Dumitru, C. D. et al. TNF-α induction by LPS is regulated posttranscriptionally via a Tpl2/ERK-dependent pathway. Cell 103, 1071-1083 (2000).  https://doi.org/10.1016/S0092-8674(00)00210-5
  8. Ghosh, S., May, M. J. & Kopp, E. B. NF-κB and Rel proteins: evolutionarily conserved mediators of immune responses. Annual review of immunology 16, 225-260 (1998).  https://doi.org/10.1146/annurev.immunol.16.1.225
  9. Choi, K.-C. et al. Intestinal anti-inflammatory activity of the seeds of Raphanus sativus L. in experimental ulcerative colitis models. Journal of ethnopharmacology 179, 55-65 (2016).  https://doi.org/10.1016/j.jep.2015.12.045
  10. Baek, S.-H., Park, M., Suh, J.-H. & Choi, H.-S. Protective effects of an extract of young radish (Raphanus sativus L) cultivated with sulfur (sulfur-radish extract) and of sulforaphane on carbon tetrachloride-induced hepatotoxicity. Bioscience, biotechnology, and biochemistry 72, 1176-1182 (2008).  https://doi.org/10.1271/bbb.70545
  11. Kook, S.-H., Choi, K.-C., Lee, Y.-H., Cho, H.-K. & Lee, J.-C. Raphanus sativus L. seeds prevent LPS-stimulated inflammatory response through negative regulation of the p38 MAPK-NF-κB pathway. International immunopharmacology 23, 726-734 (2014).  https://doi.org/10.1016/j.intimp.2014.11.001
  12. Wongrakpanich, S., Wongrakpanich, A., Melhado, K. & Rangaswami, J. A comprehensive review of non-steroidal anti-inflammatory drug use in the elderly. Aging and disease 9, 143 (2018). 
  13. Khamees, A. H. Phytochemical and pharmacological analysis for seeds of two varieties of Iraqi Raphanus sativus. Int J Pharm Sci Rev Res 43, 237-242 (2017). 
  14. Salis, K. M. & Ramabhimaiah, S. Beneficial effects of vegetable oils (Rice bran and Mustard oils) on anti-inflammatory and gastro intestinal profiles of indomethacin in rats. Biomedical and Pharmacology Journal 6, 375-379 (2015).  https://doi.org/10.13005/bpj/429
  15. Facchin, B. M. et al. Inflammatory biomarkers on an LPS-induced RAW 264.7 cell model: a systematic review and meta-analysis. Inflammation Research 71, 741-758 (2022).  https://doi.org/10.1007/s00011-022-01584-0
  16. Kaminska, B. MAPK signalling pathways as molecular targets for anti-inflammatory therapy-from molecular mechanisms to therapeutic benefits. Biochimica et Biophysica Acta (BBA)-Proteins and Proteomics 1754, 253-262 (2005). https://doi.org/10.1016/j.bbapap.2005.08.017