Animal Bioscience

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Microencapsulation of Lactobacillus plantarum MB001 and its probiotic effect on growth performance, cecal microbiome and gut integrity of broiler chickens in a tropical climate

  • Sasi Vimon (Department of Animal Husbandry, Faculty of Veterinary Science, Chulalongkorn University) ;
  • Kris Angkanaporn (Department of Veterinary Physiology, Faculty of Veterinary Science, Chulalongkorn University) ;
  • Chackrit Nuengjamnong (Department of Animal Husbandry, Faculty of Veterinary Science, Chulalongkorn University)
  • Received : 2022.11.09
  • Accepted : 2023.03.15
  • Published : 2023.08.01
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Abstract

Objective: Microencapsulation technologies have been developed and successfully applied to protect the probiotic bacterial cells damaged by environmental exposure. This study aimed to investigate the effects of microencapsulation of Lactobacillus plantarum MB001 on the growth performance, ileal nutrient digestibility, jejunal histomorphology and cecal microbiome of broiler chickens in a tropical climate. Methods: A total of 288 one-day-old female broilers (Ross 308) were randomly allocated into 4 groups (6 replicates of 12 birds). Treatments included, i) a basal diet (NC), ii) NC + avilamycin (10 mg/kg) (PC), iii) NC + non-encapsulated L. plantarum MB001 (1×108 colony-forming unit [CFU]/kg of diet) (N-LP), iv) NC + microencapsulated L. plantarum MB001 (1×108 CFU/kg of diet) (ME-LP). Results: Dietary supplementation of ME-LP improved average daily gain, and feed conversion ratio of broilers throughout the 42-d trial period (p<0.05), whereas ME-LP did not affect average daily feed intake compared with NC group. Both N-LP and ME-LP improved apparent ileal digestibility of crude protein and ether extract compared with NC group (p<0.05). The broilers fed ME-LP supplemented diet exhibited a beneficial effect on jejunal histomorphology of villus height (VH), crypt depth (CD) and villus height to crypt depth ratio (VH:CD) of broilers compared to NC group (p<0.05). At the phylum level, Firmicutes was enriched (p<0.05) and Proteobacteria was decreased (p<0.05) only in the ME-LP group. At the genus level, the ME-LP diets increased (p<0.05) the number of both Lactobacillus and Enterococcus compared to NC, PC, and N-LP groups (p<0.05). Conclusion: Microencapsulation assists the efficient functioning of probiotics. ME-LP could be potentially used as a feed additive for improvement of cecal microbiota, gut integrity and nutrient utilization, leading to better performance of broilers.

Keywords

Acknowledgement

This work was supported by Chulalongkorn University, Thailand Science Research and Innovation (TSRI), and KMP Biotech Company, Thailand. The authors would like to acknowledge the grant of research and researcher for industries (RRI), Thailand (No. PHD60I0005).

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