Animal Bioscience

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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The variation of insulin like growth factor 2 maker is associated with growth traits in Thai native (Kradon) pigs

  • Kessara Ampaporn (Department of Animal Science, Faculty of Natural Resources, Rajamangala University of Technology Isan Sakon Nakon Campus) ;
  • Rattikan Suwannasing (Department of Animal Science, Faculty of Natural Resources, Rajamangala University of Technology Isan Sakon Nakon Campus) ;
  • Pitchayanipa Phongphanich (Animal Production and Management Division, Faculty of Natural Resources, Prince of Songkla University) ;
  • Supanon Tunim (Animal Production and Management Division, Faculty of Natural Resources, Prince of Songkla University) ;
  • Monchai Duangjinda (Department of Animal Science, Faculty of Agriculture, Khon Kaen University)
  • Received : 2022.11.11
  • Accepted : 2023.05.01
  • Published : 2023.09.01
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Abstract

Objective: This study was conducted to investigate polymorphisms of the melanocortin-4 receptor (MC4R) and insulin like growth factor 2 (IGF2) genes and to evaluate the growth traits affected by such polymorphisms in Thai native (Kradon) pigs. Methods: Blood samples and productive data from 91 Kradon pigs were collected. DNA was extracted and quantified, the IGF2 and MC4R genes were amplified, and the polymerase chain reaction (PCR) produces were digested using the PCR-restriction fragment length polymorphism (PCR-RFLP) technique. Genotyping was performed, and the association between genotypes and growth traits on the birth and weaning weights were evaluated. Results: The IGF2 intron7 g.162G>C variations in Kradon pigs were found in three genotypes: i) GG, ii) GC, and iii) CC. The GG genotype frequency was the highest followed by the GC and CC genotypes. The frequencies of the G and C alleles were 0.703 and 0.297, respectively. The MC4R genotype was found in only one genotype (GG). The IGF2 gene pattern was not associated with birth weight traits, whereas the IGF2 gene pattern was related to the weaning weight trait in Kradon pigs. Pigs with the CC and GC genotypes had higher weaning weights than ones with the GG genotype (p<0.001). Conclusion: Thai native Kradon pigs with the CC and GC genotypes of the IGF2 gene have higher weaning weights than pigs with the GG genotype.

Keywords

Acknowledgement

The research was supported by animal blood samples from the Rajamangala University of Technology Isan, Sakon Nakon Campus. The authors are grateful to the Breeding and Omics Laboratory, Northeastern Science Park (Khon Kaen), Thailand, and especially thank the Animal Biotechnology Laboratory, Animal Production and Management Division, Faculty of Natural Resources, Prince of Songkla University, Songkla, Thailand.

References

  1. Falvey L. Research on native pigs in Thailand. Rome, Italy: World Animal Review; 1982.
  2. Vasupen K. Nutrition studies in native, Thai Kadon pig [Ph.D. Thesis]. Utrecht, The Netherlands: Department of Nutrition, Faculty of Veterinary Medicine, Utrecht University; 2007.
  3. Rattanronchart S. Thai native pigs [internet]. c2021 [cited 2021 Dec 9]. Available from: https://www.angelfire.com
  4. Amarger V, Nguyen M, Van Laere AS, et al. Comparative sequence analysis of the INS-IGF2-H19 gene cluster in pigs. Mamm Genome 2002;13:388-98. https://doi.org/10.1007/s00335-001-3059-x
  5. O' Dell SD, Day INM. Molecules in focus Insulin-like growth factor II (IGF-II). Int J Biochem Cell Biol 1998;30:767-71. https://doi.org/10.1016/S1357-2725(98)00048-X
  6. Kostyunina OV, Kramarenko SS, Svezhentseva NA, Sizareva EI, Zinovieva NA. The association of IGF2 with productive traits of pigs of large white breed in the aspect of sexual differentiation. Agric Biol 2015;50:736-45. https://doi.org/10.15389/agrobiology.2015.6.736eng
  7. Lamberson WR, Sterle JA, Matteri RL. Relationships of serum insulin-like growth factor II concentrations to growth, compositional, and reproductive traits of swine. J Anim Sci 1996;74:17536. https://doi.org/10.2527/1996.7481753x
  8. Burgos C, Galve A, Moreno C, et al. The effects of two alleles of IGF2 on fat content in pig carcasses and pork. Meat Sci 2012;90:309-13. https://doi.org/10.1016/j.meatsci.2011.07.016
  9. Thengpimol P, Suwanasopee T, Mekchay S, Koonawootrittriron S. Association of the Insulin-like Growth Factor II Gene (IGF-II) with growth and body conformation traits in a commercial swine population. Khon Kaen AGR J 2009;37:319-30.
  10. Ampaporn K, Parnprasert P, Sanklong Ch, Duangjinda M. The effects of IGF-II gene on growth trait and carcass quality in Kradon pigs. Khon Kaen AGR J 2018;46:533-42.
  11. Kim KS, Larsen N, Short T, Plastow G, Rothschild MF. A missense variant of the porcine melanocortin-4 receptor (MC4R) gene is associated with fatness, growth, and feed intake traits. Mamm Genome 2000;11:131-5. https://doi.org/10.1007/s003350010025
  12. Meidtner K, Wermter AK, Hinney A, Remschmidt H, Hebebrand J, Fries R. Association of the melanocortin 4 receptor with feed intake and daily gain in F2 Mangalitsa x Pie'train pigs. Anim Genet 2006;37:245-7. https://doi.org/10.1111/j.1365-2052.2006.01414.x
  13. Bruun CS, Jorgensen CB, Nielsen VH, Andersson L, Fredholm M. Evaluation of the porcine melanocortin 4 receptor (MC4R) gene as a positional candidate for a fatness QTL in a cross between Landrace and Hampshire. Anim Genet 2006;37:359-62. https://doi.org/10.1111/j.13652052.2006.01488.x
  14. Goodwin W, Linacre A, Hadi S. An introduction to forensic genetics. Chicheste, UK: John Wiley & Sons Ltd.; 2007.
  15. Vykoukalova Z, Knoll A, Dvorak J, Cepica S. New SNPs in the IGF2 gene and association between this gene and backfat thickness and lean meat content in Large White pigs. J Anim Breed Genet 2006;123:204-7. https://doi.org/10.1111/j.1439-0388.2006.00580.x
  16. Dvorakova V, Stupka R, Sprysl M, et al. Effect of missense mutation Asp298Asn in MC4R on growth and fatness traits in commercial pig crosses in the Czech Republic. Czech J Anim Sci 2011;56:176-80. https://doi.org/10.17221/1305-CJAS
  17. Nei M. Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics 1978;89:583-90. https://doi.org/10.1093/genetics/89.3.583
  18. Botstein D, White RL, Skolnick M, Davis RW. Construction of genetic linkage map in man using restriction fragment length polymorphisms. Am J Hum Genet 1980;32:314-31.
  19. Klomtong P, Chaweewan K, Phasuk Y, Duangjinda M. MC1R, KIT, IGF2, and NR6A1 as markers for genetic differentiation in Thai native, wild boars, and Duroc and Chinese Meishan pigs. Genet Mol Res 2015;14:12723-32. https://doi.org/10.4238/2015.October.19.16
  20. Oczkowicz M, Tyra M, Ropka-Molik K, Mucha A, Zukowski K. Effect of IGF2 of intron3-g.3072G>A on intramuscular fat (IMF) content in pigs raise in Poland. Livest Sci 2012;149:301-4. https://doi.org/10.1016/j.livsci.2012.06.021
  21. Clark DL, Clark DI, Beever JE, Dilger AC. Increased prenatal IGF2 expression due to the porcine IGF2 intron3-G3072A mutation may be responsible for increased muscle mass. J Anim Sci 2015;93:2546-58. https://doi.org/10.2527/jas.2014-8389
  22. Aslan O, Hamill RM, Davey G, et al. Variation in the IGF2 gene promoter region is associated with intramuscular fat content in porcine skeletal muscle. Mol Biol Rep 2012;39:4101-10. https://doi.org/10.1007/s11033-011-1192-5
  23. Zhao H, Wu M, Liu S, et al. Liver expression of IGF2 and related proteins in ZBED6 gene-edited pig by RNA-seq. Animals 2020;10:2184. https://doi.org/10.3390/ani10112184
  24. Younis S, Schonke M, Massart J, et al. The ZBED6-IGF2 axis has a major effect on growth of skeletal muscle and internal organs in placental mammals. Proc Natl Acad Sci 2018;115:e2048-57. https://doi.org/10.1073/pnas.1719278115
  25. Criado-Mesas L, Ballester M, Crespo-Piazuelo D, et al. Analysis of porcine IGF2 gene expression in adipose tissue and its effect on fatty acid composition. PLoS ONE 2019;14:e0220708. https://doi.org/10.1371/journal.pone.0220708
  26. Braunschweig MH. Biallelic transcription of the porcine IGF2R gene. Gene 2012;500:181-5. https://doi.org/10.1016/j.gene.2012.03.059
  27. Xiang G, Ren J, Hai T, et al. Editing porcine IGF2 regulatory element improved meat production in Chinese Bama pigs. Cell Mol Life Sci 2018;75:4619-28. https://doi.org/10.1007/s00018-018-2917-6
  28. Ruan GR, Xiang YY, Fan Y, et al. Genetic variation at RYR1, IGF2, FUT1, MUC13, and KPL2 mutations affecting production traits in Chinese commercial pig breeds. Czech J Anim Sci 2013;58:65-70. https://doi.org/10.17221/6616-CJAS
  29. Chaweewan K, Srisuriya V, Jumparat V, Nakavisut S. Association of the melanocortin-4 receptor (MC4R) with economic traits in pigs. Khon Kaen AGR J 2012;40(Suppl 2):343-50.
  30. Fan B, Onteru SK, Plastow GS, Rothschild MF. Detailed characterization of the porcine MC4R gene in relation to fatness and growth. Anim Genet 2009;40:401-9. https://doi.org/10.1111/j.1365-2052.2009.01853.x
  31. Szyndler-Nedza M, Tyra M, Blicharski T, Piorkowska K. Effect of mutation in MC4R gene on carcass quality in Pulawska pig included in conservation breeding programme. Anim Sci Pap Rep 2010;28:37-45.
  32. Piorkowska K, Tyra M, Rogoz M, Ropka-Molik K, Oczkowicz M, Rozyck M. Association of the melanocortin-4 receptor (MC4R) with feed intake, growth, fatness and carcass composition in pigs raised in Poland. Meat Sci 2010;85:297-301. https://doi.org/10.1016/j.meatsci.2010.01.017
  33. Van den MK, Stinckens A, Claeys E, et al. The Asp298Asn missense mutation in the porcine melanocortin-4 receptor (MC4R) gene can be used to affect growth and carcass traits without an effect on meat quality. Animal 2007;1:1089-98. https://doi.org/10.1017/S1751731107000456